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A conserved motif in three viral movement proteins from different genera is required for host factor recruitment and cell-to-cell movement

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A conserved motif in three viral movement proteins from different genera is required for host factor recruitment and cell-to-cell movement

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dc.contributor.author NAVARRO BOHIGUES, JOSE ANTONIO es_ES
dc.contributor.author Serra-Soriano, Marta es_ES
dc.contributor.author Corachán Valencia, Lorena es_ES
dc.contributor.author Pallás Benet, Vicente es_ES
dc.date.accessioned 2021-11-05T12:37:03Z
dc.date.available 2021-11-05T12:37:03Z
dc.date.issued 2020-03-16 es_ES
dc.identifier.issn 2045-2322 es_ES
dc.identifier.uri http://hdl.handle.net/10251/176118
dc.description.abstract [EN] Due to their minimal genomes, plant viruses are forced to hijack specific cellular pathways to ensure host colonization, a condition that most frequently involves physical interaction between viral and host proteins. Among putative viral interactors are the movement proteins, responsible for plasmodesma gating and genome binding during viral transport. Two of them, DGBp1 and DGBp2, are required for alpha-, beta- and gammacarmovirus cell-to-cell movement, but the number of DGBp-host interactors identified at present is limited. By using two different approaches, yeast two-hybrid and bimolecular fluorescence complementation assays, we found three Arabidopsis factors, eIF3g1, RPP3A and WRKY36, interacting with DGBp1s from each genus mentioned above. eIF3g1 and RPP3A are mainly involved in protein translation initiation and elongation phases, respectively, while WRKY36 belongs to WRKY transcription factor family, important regulators of many defence responses. These host proteins are not expected to be associated with viral movement, but knocking out WRKY36 or silencing either RPP3A or eIF3g1 negatively affected Arabidopsis infection by Turnip crinkle virus. A highly conserved FNF motif at DGBp1 C-terminus was required for protein-protein interaction and cell-to-cell movement, suggesting an important biological role. es_ES
dc.description.sponsorship We thank Dr. Anne Simon and Dr. Steve A. Lommel for providing an infectious cDNA clone of the Turnip crinkle virus strain M (TCV-M) and PZP-TCV-sGFP plasmid, respectively. This work was funded by grant BIO2017-88321-R from the Spanish Agencia Estatal de Investigacion (AEI) and Fondo Europeo de Desarrollo Regional (FEDER). J.A.N. and M.S.-S. are the recipients of a postdoctoral contract and a PhD fellowship from the Ministerio de Ciencia, Innovacion y Universidades of Spain. es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Scientific Reports es_ES
dc.rights Reconocimiento (by) es_ES
dc.title A conserved motif in three viral movement proteins from different genera is required for host factor recruitment and cell-to-cell movement es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/s41598-020-61741-5 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI//BIO2017-88321-R//DESCRIFRANDO INTERACCIONES VIRUS-PLANTA ESENCIALES PARA LA SUSCEPTIBILIDAD Y/O RESISTENCIA EN DOS PATOSISTEMAS AGRONOMICAMENTE RELEVANTES/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Navarro Bohigues, JA.; Serra-Soriano, M.; Corachán Valencia, L.; Pallás Benet, V. (2020). A conserved motif in three viral movement proteins from different genera is required for host factor recruitment and cell-to-cell movement. Scientific Reports. 10(1):1-15. https://doi.org/10.1038/s41598-020-61741-5 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/s41598-020-61741-5 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 15 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 10 es_ES
dc.description.issue 1 es_ES
dc.identifier.pmid 32179855 es_ES
dc.identifier.pmcid PMC7075923 es_ES
dc.relation.pasarela S\433586 es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Ciencia, Innovación y Universidades es_ES
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