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A dual function of SnRK2 kinases in the regulation of SnRK1 and plant growth

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A dual function of SnRK2 kinases in the regulation of SnRK1 and plant growth

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dc.contributor.author Belda-Palazón, Borja es_ES
dc.contributor.author Adamo, Mattia es_ES
dc.contributor.author Valerio, Concetta es_ES
dc.contributor.author Ferreira, Liliana J. es_ES
dc.contributor.author Confraria, Ana es_ES
dc.contributor.author Reis-Barata, Diana es_ES
dc.contributor.author Rodrigues, Americo es_ES
dc.contributor.author Meyer, Christian es_ES
dc.contributor.author Rodríguez Egea, Pedro Luís es_ES
dc.contributor.author Baena-González, Elena es_ES
dc.date.accessioned 2021-11-05T14:06:20Z
dc.date.available 2021-11-05T14:06:20Z
dc.date.issued 2020-11 es_ES
dc.identifier.uri http://hdl.handle.net/10251/176233
dc.description.abstract [EN] Adverse environmental conditions trigger responses in plants that promote stress tolerance and survival at the expense of growth(1). However, little is known of how stress signalling pathways interact with each other and with growth regulatory components to balance growth and stress responses. Here, we show that plant growth is largely regulated by the interplay between the evolutionarily conserved energy-sensing SNF1-related protein kinase 1 (SnRK1) protein kinase and the abscisic acid (ABA) phytohormone pathway. While SnRK2 kinases are main drivers of ABA-triggered stress responses, we uncover an unexpected growth-promoting function of these kinases in the absence of ABA as repressors of SnRK1. Sequestration of SnRK1 by SnRK2-containing complexes inhibits SnRK1 signalling, thereby allowing target of rapamycin (TOR) activity and growth under optimal conditions. On the other hand, these complexes are essential for releasing and activating SnRK1 in response to ABA, leading to the inhibition of TOR and growth under stress. This dual regulation of SnRK1 by SnRK2 kinases couples growth control with environmental factors typical for the terrestrial habitat and is likely to have been critical for the water-to-land transition of plants. es_ES
dc.description.sponsorship We thank J.-K. Zhu for the snrk2 mutants, M. Bennett for the SnRK2.2-GFP line, C. Koncz for the SnRK1-GFP line, X. Li for the SnRK2.3-FLAG OE line, J. Schroeder for the GFP-His-FLAG and SnRK2.6-His-FLAG OE lines, C. Mackintosh for the TPS5 antibody and the Nottingham Arabidopsis stock centre for T-DNA mutant seeds. The IGC Plant Facility (Vera Nunes) is thanked for excellent plant care. This work was supported by Fundacao para a Ciencia e a Tecnologia through the R&D Units UIDB/04551/2020 (GREEN-IT-Bioresources for Sustainability) and UID/MAR/04292/2019, FCT project nos. PTDC/BIA-PLA/7143/2014, LISBOA-01-0145-FEDER-028128 and PTDC/BIA-BID/32347/2017, and FCT fellowships/contract nos. SFRH/BD/122736/2016 (M.A.), SFRH/BPD/109336/2015 (A.C.), PD/BD/150239/2019 (D.R.B.), and IF/00804/2013 (E.B.G.). Work in P.L.R.'s laboratory was funded by MCIU grant no. BIO2017-82503-R. C.M. thanks the LabEx Paris Saclay Plant Sciences-SPS (ANR-10-LABX-040-SPS) for support. B.B.P. was funded by Programa VALi+d GVA APOSTD/2017/039. This project has received funding from the European Union Horizon 2020 research and innovation programme (grant agreement no. 867426-ABA-GrowthBalance-H2020-WF-2018-2020/H2020-WF-01-2018, awarded to B.B.P.). This work is dedicated to the memory of our beloved friend and colleague Americo Rodrigues. es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Nature Plants (Online) es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title A dual function of SnRK2 kinases in the regulation of SnRK1 and plant growth es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/s41477-020-00778-w es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/867426/EU/Growth balance regulation by SnRK1 under ABA-stress conditions/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//UID%2FMAR%2F04292%2F2019/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//PTDC%2FBIA-PLA%2F7143%2F2014/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//LISBOA-01-0145-FEDER-028128/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//PTDC%2FBIA-BID%2F32347%2F2017/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F122736%2F2016/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F109336%2F2015/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//PD%2FBD%2F150239%2F2019/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//IF%2F00804%2F2013/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/SPS//ANR-10-LABX-0040-SPS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//APOSTD%2F2017%2F039/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI//BIO2017-82503-R//REGULACION DE LA SEÑALIZACION DEL ABA Y TOLERANCIA A SEQUIA MEDIANTE E3 UBIQUITIN LIGASAS QUE REGULAN EL RECAMBIO DE RECEPTORES Y FOSFATASAS 2C/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//UIDB%2F04551%2F2020//GREEN-IT-Bioresources for Sustainability/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Belda-Palazón, B.; Adamo, M.; Valerio, C.; Ferreira, LJ.; Confraria, A.; Reis-Barata, D.; Rodrigues, A.... (2020). A dual function of SnRK2 kinases in the regulation of SnRK1 and plant growth. Nature Plants (Online). 6(11):1345-1353. https://doi.org/10.1038/s41477-020-00778-w es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/s41477-020-00778-w es_ES
dc.description.upvformatpinicio 1345 es_ES
dc.description.upvformatpfin 1353 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 6 es_ES
dc.description.issue 11 es_ES
dc.identifier.eissn 2055-0278 es_ES
dc.identifier.pmid 33077877 es_ES
dc.relation.pasarela S\433482 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Saclay Plant Sciences es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Fundação para a Ciência e a Tecnologia, Portugal es_ES
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