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The res (restored cell structure by salinity) tomato mutant reveals the role of the DEAD-box RNA helicase SlDEAD39 in plant development and salt response

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The res (restored cell structure by salinity) tomato mutant reveals the role of the DEAD-box RNA helicase SlDEAD39 in plant development and salt response

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dc.contributor.author Capel, Carmen es_ES
dc.contributor.author Albaladejo, Irene es_ES
dc.contributor.author Egea, Isabel es_ES
dc.contributor.author Massaretto, Isabel L. es_ES
dc.contributor.author Yuste-Lisbona, Fernando J. es_ES
dc.contributor.author Pineda Chaza, Benito José es_ES
dc.contributor.author García Sogo, Begoña es_ES
dc.contributor.author Angosto, Trinidad es_ES
dc.contributor.author Flores, Francisco B. es_ES
dc.contributor.author Moreno Ferrero, Vicente es_ES
dc.contributor.author Lozano, Rafael es_ES
dc.contributor.author Bolarin, Maria C. es_ES
dc.contributor.author Capel, Juan es_ES
dc.date.accessioned 2021-11-05T14:11:25Z
dc.date.available 2021-11-05T14:11:25Z
dc.date.issued 2020-07 es_ES
dc.identifier.issn 0140-7791 es_ES
dc.identifier.uri http://hdl.handle.net/10251/176457
dc.description.abstract [EN] Increasing evidences highlight the importance of DEAD-box RNA helicases in plant development and stress responses. In a previous study, we characterized the tomato res mutant (restored cell structure by salinity), showing chlorosis and development alterations that reverted under salt-stress conditions. Map-based cloning demonstrates that RES gene encodes SlDEAD39, a chloroplast-targeted DEAD-box RNA helicase. Constitutive expression of SlDEAD39 complements the res mutation, while the silencing lines had a similar phenotype than res mutant, which is also reverted under salinity. Functional analysis of res mutant proved SlDEAD39 is involved in the in vivo processing of the chloroplast, 23S rRNA, at the hidden break-B site, a feature also supported by in vitro binding experiments of the protein. In addition, our results show that other genes coding for chloroplast-targeted DEAD-box proteins are induced by salt-stress, which might explain the rescue of the res mutant phenotype. Interestingly, salinity restored the phenotype of res adult plants by increasing their sugar content and fruit yield. Together, these results propose an unprecedented role of a DEAD-box RNA helicase in regulating plant development and stress response through the proper ribosome and chloroplast functioning, which, in turn, represents a potential target to improve salt tolerance in tomato crops es_ES
dc.description.sponsorship Secretaria de Estado de Investigacion, Desarrollo e Innovacion, Grant/Award Numbers: AGL2015-64991-C3-1-R, AGL2015-64991-C3-2-R, AGL2015-64991-C3-3-R, AGL2017-88702-C2-1-R es_ES
dc.language Inglés es_ES
dc.publisher Blackwell Publishing es_ES
dc.relation.ispartof Plant Cell & Environment es_ES
dc.rights Reconocimiento - No comercial - Compartir igual (by-nc-sa) es_ES
dc.subject Chloroplast-targeted DEAD-box RNA helicases es_ES
dc.subject Fruit yield es_ES
dc.subject Plant development es_ES
dc.subject RRNA processing es_ES
dc.subject Salt stress response es_ES
dc.subject Solanum lycopersicum es_ES
dc.subject Tomato es_ES
dc.subject.classification GENETICA es_ES
dc.title The res (restored cell structure by salinity) tomato mutant reveals the role of the DEAD-box RNA helicase SlDEAD39 in plant development and salt response es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/pce.13776 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88702-C2-1-R/ES/ANALISIS GENOMICO DEL DESARROLLO DE TRICOMAS COMO ESTRATEGIA PARA LA MEJORA GENETICA DE LA RESISTENCIA A PLAGAS EN TOMATE/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110833RB-C32/ES/EDICION DE PROMOTORES DE GENES QUE REGULAN EL DESARROLLO REPRODUCTIVO EN TOMATE COMO ESTRATEGIA PARA MANTENER LA PRODUCCION EN CONDICIONES DE ESTRES ABIOTICO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2015-64991-C3-1-R/ES/GENOMICA FUNCIONAL Y MEJORA GENETICA DE LA PRODUCTIVIDAD DE TOMATE: IMPORTANCIA AGRONOMICA DEL BALANCE DESARROLLO-ESTRES ABIOTICO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2015-64991-C3-2-R/ES/GENOMICA FUNCIONAL Y MEJORA GENETICA DE TOMATE: IMPORTANCIA AGRONOMICA DEL BALANCE DESARROLLO - ESTRES ABIOTICO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2015-64991-C3-3-R/ES/GENOMICA FUNCIONAL Y MEJORA GENETICA DE TOMATE: IMPORTANCIA AGRONOMICA DEL BALANCE DESARROLLO - ESTRES ABIOTICO/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Capel, C.; Albaladejo, I.; Egea, I.; Massaretto, IL.; Yuste-Lisbona, FJ.; Pineda Chaza, BJ.; García Sogo, B.... (2020). The res (restored cell structure by salinity) tomato mutant reveals the role of the DEAD-box RNA helicase SlDEAD39 in plant development and salt response. Plant Cell & Environment. 43(7):1722-1739. https://doi.org/10.1111/pce.13776 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1111/pce.13776 es_ES
dc.description.upvformatpinicio 1722 es_ES
dc.description.upvformatpfin 1739 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 43 es_ES
dc.description.issue 7 es_ES
dc.identifier.pmid 32329086 es_ES
dc.identifier.pmcid PMC7384196 es_ES
dc.relation.pasarela S\423517 es_ES
dc.contributor.funder AGENCIA ESTATAL DE INVESTIGACION es_ES
dc.contributor.funder MINISTERIO DE ECONOMIA Y EMPRESA es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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