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dc.contributor.author | Puchalt-Rodríguez, Joan Carles | es_ES |
dc.contributor.author | Layana-Castro, Pablo Emmanuel | es_ES |
dc.contributor.author | Sánchez Salmerón, Antonio José | es_ES |
dc.date.accessioned | 2021-11-05T14:12:15Z | |
dc.date.available | 2021-11-05T14:12:15Z | |
dc.date.issued | 2020-11 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/176496 | |
dc.description.abstract | [EN] Nowadays, various artificial vision-based machines automate the lifespan assays of C. elegans. These automated machines present wider variability in results than manual assays because in the latter worms can be poked one by one to determine whether they are alive or not. Lifespan machines normally use a "dead or alive criterion" based on nematode position or pose changes, without poking worms. However, worms barely move on their last days of life, even though they are still alive. Therefore, a long monitoring period is necessary to observe motility in order to guarantee worms are actually dead, or a stimulus to prompt worm movement is required to reduce the lifespan variability measure. Here, a new automated vibrotaxis-based method for lifespan machines is proposed as a solution to prompt a motion response in all worms cultured on standard Petri plates in order to better distinguish between live and dead individuals. This simple automated method allows the stimulation of all animals through the whole plate at the same time and intensity, increasing the experiment throughput. The experimental results exhibited improved live-worm detection using this method, and most live nematodes (>93%) reacted to the vibration stimulus. This method increased machine sensitivity by decreasing results variance by approximately one half (from +/- 1 individual error per plate to +/- 0.6) and error in lifespan curve was reduced as well (from 2.6% to 1.2%). | es_ES |
dc.description.sponsorship | This study was also supported by the Universitat Politecnica de Valencia with Project 20170020-UPV, Plan Nacional de I+D with Project RTI2018-094312-B-I00 and by European FEDER funds. ADM Nutrition, Biopolis SL and Archer Daniels Midland provided support in the supply of C. elegans. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI AG | es_ES |
dc.relation.ispartof | Sensors | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Image processing | es_ES |
dc.subject | Lifespan automation | es_ES |
dc.subject | C. elegans | es_ES |
dc.subject.classification | INGENIERIA DE SISTEMAS Y AUTOMATICA | es_ES |
dc.title | Reducing Results Variance in Lifespan Machines: An Analysis of the Influence of Vibrotaxis on Wild-Type Caenorhabditis elegans for the Death Criterion | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/s20215981 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/UPV//20170020-UPV/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI//RTI2018-094312-B-I00-AR//MONITORIZACION AVANZADA DE COMPORTAMIENTOS DE CAENORHABDITIS ELEGANS, BASADA EN VISION ACTIVA, PARA ANALIZAR FUNCION COGNITIVA Y ENVEJECIMIENTO/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Ingeniería de Sistemas y Automática - Departament d'Enginyeria de Sistemes i Automàtica | es_ES |
dc.description.bibliographicCitation | Puchalt-Rodríguez, JC.; Layana-Castro, PE.; Sánchez Salmerón, AJ. (2020). Reducing Results Variance in Lifespan Machines: An Analysis of the Influence of Vibrotaxis on Wild-Type Caenorhabditis elegans for the Death Criterion. Sensors. 20(21):1-17. https://doi.org/10.3390/s20215981 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/s20215981 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 17 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 20 | es_ES |
dc.description.issue | 21 | es_ES |
dc.identifier.eissn | 1424-8220 | es_ES |
dc.identifier.pmid | 33105730 | es_ES |
dc.identifier.pmcid | PMC7660079 | es_ES |
dc.relation.pasarela | S\421080 | es_ES |
dc.contributor.funder | AGENCIA ESTATAL DE INVESTIGACION | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Universitat Politècnica de València | es_ES |
dc.subject.ods | 03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades | es_ES |