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Engineered axon tracts within tubular biohybrid scaffolds

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Engineered axon tracts within tubular biohybrid scaffolds

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dc.contributor.author Rodriguez Doblado, Laura es_ES
dc.contributor.author Martínez-Ramos, Cristina es_ES
dc.contributor.author García-Verdugo, José Manuel es_ES
dc.contributor.author Moreno-Manzano, Victoria es_ES
dc.contributor.author Monleón Pradas, Manuel es_ES
dc.date.accessioned 2021-12-30T19:27:05Z
dc.date.available 2021-12-30T19:27:05Z
dc.date.issued 2021-08-12 es_ES
dc.identifier.issn 1741-2560 es_ES
dc.identifier.uri http://hdl.handle.net/10251/179190
dc.description.abstract [EN] Injuries to the nervous system that involve the disruption of axonal pathways are devastating to the individual and require specific tissue engineering strategies. Here we analyse a cells-biomaterials strategy to overcome the obstacles limiting axon regeneration in vivo, based on the combination of a hyaluronic acid (HA) single-channel tubular conduit filled with poly-L-lactide acid (PLA) fibres in its lumen, with pre-cultured Schwann cells (SCs) as cells supportive of axon extension. The HA conduit and PLA fibres sustain the proliferation of SC, which enhance axon growth acting as a feeder layer and growth factor pumps. The parallel unidirectional ensemble formed by PLA fibres and SC tries to recapitulate the directional features of axonal pathways in the nervous system. A dorsal root ganglion (DRG) explant is planted on one of the conduit's ends to follow axon outgrowth from the DRG. After a 21 d co-culture of the DRG + SC-seeded conduit ensemble, we analyse the axonal extension throughout the conduit by scanning, transmission electronic and confocal microscopy, in order to study the features of SC and the grown axons and their association. The separate effects of SC and PLA fibres on the axon growth are also experimentally addressed. The biohybrid thus produced may be considered a synthetic axonal pathway, and the results could be of use in strategies for the regeneration of axonal tracts. es_ES
dc.description.sponsorship The authors acknowledge financing from the Spanish Ministry of Economy and Competitiveness through MINECO grants, Funded by AEI 'RTI2018-095872-B-C21 and C22/ERDF', the Valencian Council for Innovation, Universities, Science and Digital Society (PROMETEO/2019/075 to J.M.G-V,) and the Spanish Cell Therapy Network (TerCel-RD16/0011/0026 to J.M.G-V.). Laura Rodríguez Doblado acknowledges scholarship FPU15/04975 of the Spanish Ministry of Education, Culture and Sports. We thank the Microscopy Service at the UPV, where the FESEM images were obtained and Patricia García-Tarraga for her technical support. es_ES
dc.language Inglés es_ES
dc.publisher IOP Publishing es_ES
dc.relation.ispartof Journal of Neural Engineering es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Schwann cell culture es_ES
dc.subject Axon tract es_ES
dc.subject Dorsal root ganglion cell culture es_ES
dc.subject Hyaluronic acid conduit es_ES
dc.subject Poly-lactic fibres es_ES
dc.subject.classification TERMODINAMICA APLICADA (UPV) es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title Engineered axon tracts within tubular biohybrid scaffolds es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1088/1741-2552/ac17d8 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095872-B-C22/ES/NUEVO DISPOSITIVO BIOACTIVO PARA LA REGENERACION DE LESIONES DE LA MEDULA ESPINAL./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FEDER//C22%2FERDF/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MECD//FPU15%2F04975/ES/FPU15%2F04975/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2019%2F075/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//RD16%2F0011%2F0026/ES/Red de Terapia Celular (TerCel)/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Rodriguez Doblado, L.; Martínez-Ramos, C.; García-Verdugo, JM.; Moreno-Manzano, V.; Monleón Pradas, M. (2021). Engineered axon tracts within tubular biohybrid scaffolds. Journal of Neural Engineering. 18(4):1-15. https://doi.org/10.1088/1741-2552/ac17d8 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1088/1741-2552/ac17d8 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 15 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 18 es_ES
dc.description.issue 4 es_ES
dc.identifier.pmid 34311448 es_ES
dc.relation.pasarela S\418564 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder MINISTERIO DE EDUCACION es_ES
dc.contributor.funder AGENCIA ESTATAL DE INVESTIGACION es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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