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Symptom Severity, Infection Progression and Plant Responses in Solanum Plants Caused by Three Pospiviroids Vary with the Inoculation Procedure

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Symptom Severity, Infection Progression and Plant Responses in Solanum Plants Caused by Three Pospiviroids Vary with the Inoculation Procedure

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dc.contributor.author Vázquez Prol, Francisco es_ES
dc.contributor.author Márquez-Molins, Joan es_ES
dc.contributor.author Rodrigo Bravo, Ismael es_ES
dc.contributor.author López-Gresa, María Pilar es_ES
dc.contributor.author Belles Albert, José Mª es_ES
dc.contributor.author Gomez, Gustavo Germán es_ES
dc.contributor.author Pallás Benet, Vicente es_ES
dc.contributor.author Lisón, Purificación es_ES
dc.date.accessioned 2022-02-09T19:04:51Z
dc.date.available 2022-02-09T19:04:51Z
dc.date.issued 2021-06-08 es_ES
dc.identifier.uri http://hdl.handle.net/10251/180674
dc.description.abstract [EN] Infectious viroid clones consist of dimeric cDNAs used to generate transcripts which mimic the longer-than-unit replication intermediates. These transcripts can be either generated in vitro or produced in vivo by agro-inoculation. We have designed a new plasmid, which allows both inoculation methods, and we have compared them by infecting Solanum lycopersicum and Solanum melongena with clones of Citrus exocortis virod (CEVd), Tomato chlorotic dwarf viroid (TCDVd), and Potato spindle tuber viroid (PSTVd). Our results showed more uniform and severe symptoms in agro-inoculated plants. Viroid accumulation and the proportion of circular and linear forms were different depending on the host and the inoculation method and did not correlate with the symptoms, which correlated with an increase in PR1 induction, accumulation of the defensive signal molecules salicylic (SA) and gentisic (GA) acids, and ribosomal stress in tomato plants. The alteration in ribosome biogenesis was evidenced by both the upregulation of the tomato ribosomal stress marker SlNAC082 and the impairment in 18S rRNA processing, pointing out ribosomal stress as a novel signature of the pathogenesis of nuclear-replicating viroids. In conclusion, this updated binary vector has turned out to be an efficient and reproducible method that will facilitate the studies of viroid¿host interactions es_ES
dc.description.sponsorship This work was supported by the Spanish Ministry of Economy and Competitiveness (co-supported by FEDER) Grants BIO2017-88321-R (V.P.) and PID2019-104126RB-I00 (G.G.). The funders had no role in the experiment design, data analysis, decision to publish, or preparation of the manuscript es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof International Journal of Molecular Sciences es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Pospiviroidae es_ES
dc.subject CEVd es_ES
dc.subject TCDVd es_ES
dc.subject PSTVd es_ES
dc.subject Agro-infiltration es_ES
dc.subject Transcript es_ES
dc.subject Eggplant es_ES
dc.subject Tomato es_ES
dc.subject Ribosome es_ES
dc.subject Stress es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Symptom Severity, Infection Progression and Plant Responses in Solanum Plants Caused by Three Pospiviroids Vary with the Inoculation Procedure es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/ijms22126189 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-88321-R/ES/DESCRIFRANDO INTERACCIONES VIRUS-PLANTA ESENCIALES PARA LA SUSCEPTIBILIDAD Y%2FO RESISTENCIA EN DOS PATOSISTEMAS AGRONOMICAMENTE RELEVANTES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104126RB-I00/ES/REDES REGULADORAS GENICAS INFLUENCIADAS POR EL AMBIENTE IMPLICADAS EN EL CONTROL DE LA REPUESTA A ESTRES EN MELON/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Vázquez Prol, F.; Márquez-Molins, J.; Rodrigo Bravo, I.; López-Gresa, MP.; Belles Albert, JM.; Gomez, GG.; Pallás Benet, V.... (2021). Symptom Severity, Infection Progression and Plant Responses in Solanum Plants Caused by Three Pospiviroids Vary with the Inoculation Procedure. International Journal of Molecular Sciences. 22(12):1-16. https://doi.org/10.3390/ijms22126189 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/ijms22126189 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 16 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 22 es_ES
dc.description.issue 12 es_ES
dc.identifier.eissn 1422-0067 es_ES
dc.identifier.pmid 34201240 es_ES
dc.identifier.pmcid PMC8273692 es_ES
dc.relation.pasarela S\439200 es_ES
dc.contributor.funder MINECO es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.subject.ods 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible es_ES
upv.costeAPC 2000 es_ES


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