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dc.contributor.author | Andrés-Colás, Nuria | es_ES |
dc.contributor.author | Zhu, Qiang | es_ES |
dc.contributor.author | Takenaka, Mizuki | es_ES |
dc.contributor.author | De Rybel, Bert | es_ES |
dc.contributor.author | Weijers, Dolf | es_ES |
dc.contributor.author | Van Der Straeten, Dominique | es_ES |
dc.date.accessioned | 2022-02-10T19:02:24Z | |
dc.date.available | 2022-02-10T19:02:24Z | |
dc.date.issued | 2017-08-15 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/180734 | |
dc.description.abstract | [EN] Recent identification of several different types of RNA editing factors in plant organelles suggests complex RNA editosomes within which each factor has a different task. However, the precise protein interactions between the different editing factors are still poorly understood. In this paper, we show that the E+-type pentatricopeptide repeat (PPR) protein SLO2, which lacks a C-terminal cytidine deaminase-like DYW domain, interacts in vivo with the DYW-type PPR protein DYW2 and the P-type PPR protein NUWA in mitochondria, and that the latter enhances the interaction of the former ones. These results may reflect a protein scaffold or complex stabilization role of NUWA between E+-type PPR and DYW2 proteins. Interestingly, DYW2 and NUWA also interact in chloroplasts, and DYW2-GFP overexpressing lines show broad editing defects in both organelles, with predominant specificity for sites edited by E+-type PPR proteins. The latter suggests a coordinated regulation of organellar multiple site editing through DYW2, which probably provides the deaminase activity to E+ editosomes. | es_ES |
dc.description.sponsorship | N.A.-C. and D.V.D.S. thank Maria Helena de Souza Goldman for the N. benthamiana seeds and helpful suggestions regarding experimental design and data interpretation; Irina Vaseva for critical reading of the first draft of this manuscript; and Yordanka Yordanova for experimental help in genotyping the mef57 mutant line. D.V.D.S. acknowledges the Research Foundation Flanders for financial support (Project G.0C84.14N). B.D.R. was funded by a Marie Curie long-term postdoctoral fellowship (FP7-PEOPLE-2009-IEF-252503) and by The Netherlands Organization for Scientific Research (NWO; VIDI-864.13.001). D.W. acknowledges funding by NWO (ERA-CAPS Project EURO-PEC; 849.13.006). M.T. was supported by Deutsche Forschungsgemeinschaft Grants TA624/4-2, TA624/-1, and TA624/10-1. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Proceedings of the National Academy of Sciences | es_ES |
dc.relation.ispartof | Proceedings of the National Academy of Sciences of the United States of America (Online) | es_ES |
dc.rights | Reconocimiento - No comercial (by-nc) | es_ES |
dc.subject | SLO2 | es_ES |
dc.subject | DYW2 | es_ES |
dc.subject | NUWA | es_ES |
dc.subject | PPR | es_ES |
dc.subject | RNA editing | es_ES |
dc.subject.classification | BIOQUIMICA Y BIOLOGIA MOLECULAR | es_ES |
dc.title | Multiple PPR protein interactions are involved in the RNA editing system in Arabidopsis mitochondria and plastids | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1073/pnas.1705815114 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/252503/EU/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/NWO//849.13.006/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/NWO//VIDI-864.13.001/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/DFG//TA624%2F4-2/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/DFG//TA624%2F-1/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/DFG//TA624%2F10-1/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia | es_ES |
dc.description.bibliographicCitation | Andrés-Colás, N.; Zhu, Q.; Takenaka, M.; De Rybel, B.; Weijers, D.; Van Der Straeten, D. (2017). Multiple PPR protein interactions are involved in the RNA editing system in Arabidopsis mitochondria and plastids. Proceedings of the National Academy of Sciences of the United States of America (Online). 114(33):8883-8888. https://doi.org/10.1073/pnas.1705815114 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1073/pnas.1705815114 | es_ES |
dc.description.upvformatpinicio | 8883 | es_ES |
dc.description.upvformatpfin | 8888 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 114 | es_ES |
dc.description.issue | 33 | es_ES |
dc.identifier.eissn | 1091-6490 | es_ES |
dc.identifier.pmid | 28761003 | es_ES |
dc.identifier.pmcid | PMC5565447 | es_ES |
dc.relation.pasarela | S\378286 | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.contributor.funder | Deutsche Forschungsgemeinschaft | es_ES |
dc.contributor.funder | Netherlands Organization for Scientific Research | es_ES |
dc.contributor.funder | Fonds Wetenschappelijk Onderzoek - Vlaanderen Onderzoeksprojecten FWO | es_ES |