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Role of Curing Temperature of Poly(Glycerol Sebacate) Substrates on Protein-Cell Interaction and Early Cell Adhesion

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Role of Curing Temperature of Poly(Glycerol Sebacate) Substrates on Protein-Cell Interaction and Early Cell Adhesion

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dc.contributor.author Martín-Cabezuelo, Rubén es_ES
dc.contributor.author Rodriguez-Hernandez, Jose-Carlos es_ES
dc.contributor.author Vilariño, Guillermo es_ES
dc.contributor.author Vallés Lluch, Ana es_ES
dc.date.accessioned 2022-03-23T19:03:09Z
dc.date.available 2022-03-23T19:03:09Z
dc.date.issued 2021-02 es_ES
dc.identifier.uri http://hdl.handle.net/10251/181512
dc.description.abstract [EN] A novel procedure to obtain smooth, continuous polymeric surfaces from poly(glycerol sebacate) (PGS) has been developed with the spin-coating technique. This method proves useful for separating the effect of the chemistry and morphology of the networks (that can be obtained by varying the synthesis parameters) on cell-protein-substrate interactions from that of structural variables. Solutions of the PGS pre-polymer can be spin-coated, to then be cured. Curing under variable temperatures has been shown to lead to PGS networks with different chemical properties and topographies, conditioning their use as a biomaterial. Particularly, higher synthesis temperatures yield denser networks with fewer polar terminal groups available on the surface. Material-protein interactions were characterised by using extracellular matrix proteins such as fibronectin (Fn) and collagen type I (Col I), to unveil the biological interface profile of PGS substrates. To that end, atomic force microscopy (AFM) images and quantification of protein adsorbed in single, sequential and competitive protein incubations were used. Results reveal that Fn is adsorbed in the form of clusters, while Col I forms a characteristic fibrillar network. Fn has an inhibitory effect when incubated prior to Col I. Human umbilical endothelial cells (HUVECs) were also cultured on PGS surfaces to reveal the effect of synthesis temperature on cell behaviour. To this effect, early focal adhesions (FAs) were analysed using immunofluorescence techniques. In light of the results, 130 degrees C seems to be the optimal curing temperature since a preliminary treatment with Col I or a Fn:Col I solution facilitates the formation of early focal adhesions and growth of HUVECs. es_ES
dc.description.sponsorship This research was funded by the Spanish Ministerio de Economia y Competitividad, grant number DPI2015-65401-C3-2-R. A. Valles acknowledges the support of the Generalitat Valenciana, Conselleria de Educacion, Investigacion, Cultura y Deporte through project AEST/2020/052. es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Polymers es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Poly(glycerol sebacate) es_ES
dc.subject Focal adhesion es_ES
dc.subject Protein adsorption es_ES
dc.subject Polymer-protein interaction es_ES
dc.title Role of Curing Temperature of Poly(Glycerol Sebacate) Substrates on Protein-Cell Interaction and Early Cell Adhesion es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/polym13030382 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//DPI2015-65401-C3-2-R/ES/SOPORTES POLIMERICOS MULTIFUNCIONALES PARA CO-CULTIVO CELULAR INDIRECTO Y ESTIMULACION QUIMICA DESTINADOS A MIMETIZAR TEJIDO RENAL IN VITRO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//AEST%2F2020%2F052/ es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Martín-Cabezuelo, R.; Rodriguez-Hernandez, J.; Vilariño, G.; Vallés Lluch, A. (2021). Role of Curing Temperature of Poly(Glycerol Sebacate) Substrates on Protein-Cell Interaction and Early Cell Adhesion. Polymers. 13(3):1-14. https://doi.org/10.3390/polym13030382 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/polym13030382 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 14 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 13 es_ES
dc.description.issue 3 es_ES
dc.identifier.eissn 2073-4360 es_ES
dc.identifier.pmid 33530537 es_ES
dc.identifier.pmcid PMC7865911 es_ES
dc.relation.pasarela S\426426 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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