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Alginate-Agarose Hydrogels Improve the In Vitro Differentiation of Human Dental Pulp Stem Cells in Chondrocytes. A Histological Study

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Alginate-Agarose Hydrogels Improve the In Vitro Differentiation of Human Dental Pulp Stem Cells in Chondrocytes. A Histological Study

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dc.contributor.author Oliver-Ferrándiz, María es_ES
dc.contributor.author Milián, Lara es_ES
dc.contributor.author Sancho-Tello, María es_ES
dc.contributor.author Martín de Llano, José Javier es_ES
dc.contributor.author Gisbert-Roca, Fernando es_ES
dc.contributor.author Martínez-Ramos, Cristina es_ES
dc.contributor.author Carda, Carmen es_ES
dc.contributor.author Mata, Manuel es_ES
dc.date.accessioned 2022-03-24T19:03:32Z
dc.date.available 2022-03-24T19:03:32Z
dc.date.issued 2021-07 es_ES
dc.identifier.uri http://hdl.handle.net/10251/181549
dc.description.abstract [EN] Matrix-assisted autologous chondrocyte implantation (MACI) has shown promising results for cartilage repair, combining cultured chondrocytes and hydrogels, including alginate. The ability of chondrocytes for MACI is limited by different factors including donor site morbidity, dedifferentiation, limited lifespan or poor proliferation in vitro. Mesenchymal stem cells could represent an alternative for cartilage regeneration. In this study, we propose a MACI scaffold consisting of a mixed alginate-agarose hydrogel in combination with human dental pulp stem cells (hDPSCs), suitable for cartilage regeneration. Scaffolds were characterized according to their rheological properties, and their histomorphometric and molecular biology results. Agarose significantly improved the biomechanical behavior of the alginate scaffolds. Large scaffolds were manufactured, and a homogeneous distribution of cells was observed within them. Although primary chondrocytes showed a greater capacity for chondrogenic differentiation, hDPSCs cultured in the scaffolds formed large aggregates of cells, acquired a rounded morphology and expressed high amounts of type II collagen and aggrecan. Cells cultured in the scaffolds expressed not only chondral matrix-related genes, but also remodeling proteins and chondrocyte differentiation factors. The degree of differentiation of cells was proportional to the number and size of the cell aggregates that were formed in the hydrogels. es_ES
dc.description.sponsorship This work was funded by the Ministry of Economy and Competitiveness of the Spanish Government (PID2019-106099RB-C42, MM) and by the Generalitat Valenciana, Spain (PROMETEO/2020/069, CC). CIBER-BBN and CIBER-ER are financed by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and the Instituto de Salud Carlos III, with assistance of the European Regional Development Fund. es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Biomedicines es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Cartilage regeneration es_ES
dc.subject HDPSCs es_ES
dc.subject MACI es_ES
dc.subject Alginate es_ES
dc.subject Agarose es_ES
dc.subject Chondrocyte es_ES
dc.subject Tissue engineering es_ES
dc.title Alginate-Agarose Hydrogels Improve the In Vitro Differentiation of Human Dental Pulp Stem Cells in Chondrocytes. A Histological Study es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/biomedicines9070834 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106099RB-C42/ES/MICROGELES BIOMIMETICOS PARA EL ESTUDIO DEL MICROENTORNO DEL TUMOR (TME) Y LA TRANSICION EPITELIO MESENQUIMA (EMT) EN CANCER DE PULMON/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//PID2019-106099RB-C42/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F 2020%2F069/ es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Oliver-Ferrándiz, M.; Milián, L.; Sancho-Tello, M.; Martín De Llano, JJ.; Gisbert-Roca, F.; Martínez-Ramos, C.; Carda, C.... (2021). Alginate-Agarose Hydrogels Improve the In Vitro Differentiation of Human Dental Pulp Stem Cells in Chondrocytes. A Histological Study. Biomedicines. 9(7):1-22. https://doi.org/10.3390/biomedicines9070834 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/biomedicines9070834 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 22 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 9 es_ES
dc.description.issue 7 es_ES
dc.identifier.eissn 2227-9059 es_ES
dc.identifier.pmid 34356898 es_ES
dc.identifier.pmcid PMC8301309 es_ES
dc.relation.pasarela S\443630 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina es_ES


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