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Microgametophyte development in Cannabis sativa L. and first androgenesis induction through microspore embryogenesis

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Microgametophyte development in Cannabis sativa L. and first androgenesis induction through microspore embryogenesis

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dc.contributor.author Galán-Ávila, Alberto es_ES
dc.contributor.author García-Fortea, Edgar es_ES
dc.contributor.author Prohens Tomás, Jaime es_ES
dc.contributor.author Herraiz García, Francisco Javier es_ES
dc.date.accessioned 2022-04-27T06:28:00Z
dc.date.available 2022-04-27T06:28:00Z
dc.date.issued 2021-05-25 es_ES
dc.identifier.uri http://hdl.handle.net/10251/182135
dc.description.abstract [EN] Development of double haploids is an elusive current breeding objective in Cannabis sativa L. We have studied the whole process of anther and pollen grain formation during meiosis, microsporogenesis, and microgametogenesis and correlated the different microgametophyte developmental stages with bud length in plants from varieties USO31 and Finola. We also studied microspore and pollen amyloplast content and studied the effect of a cold pretreatment to excised buds prior to microspore in vitro culture. Up to 476,903 microspores and pollen grains per male flower, with in vivo microspore viability rates from 53.71 to 70.88% were found. A high uniformity in the developmental stage of microspores and pollen grains contained in anthers was observed, and this allowed the identification of bud length intervals containing mostly vacuolate microspores and young bi-cellular pollen grains. The starch presence in C. sativa microspores and pollen grains follows a similar pattern to that observed in species recalcitrant to androgenesis. Although at a low frequency, cold-shock pretreatment applied on buds can deviate the naturally occurring gametophytic pathway toward an embryogenic development. This represents the first report concerning androgenesis induction in C. sativa, which lays the foundations for double haploid research in this species. es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation.ispartof Frontiers in Plant Science es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Amyloplast es_ES
dc.subject Cold-shock bud pretreatment es_ES
dc.subject Double haploids es_ES
dc.subject Microsporogenesis es_ES
dc.subject Microgametogenesis es_ES
dc.subject Pollen embryogenesis es_ES
dc.subject.classification GENETICA es_ES
dc.title Microgametophyte development in Cannabis sativa L. and first androgenesis induction through microspore embryogenesis es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fpls.2021.669424 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Galán-Ávila, A.; García-Fortea, E.; Prohens Tomás, J.; Herraiz García, FJ. (2021). Microgametophyte development in Cannabis sativa L. and first androgenesis induction through microspore embryogenesis. Frontiers in Plant Science. 12:1-22. https://doi.org/10.3389/fpls.2021.669424 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fpls.2021.669424 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 22 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 12 es_ES
dc.identifier.eissn 1664-462X es_ES
dc.identifier.pmid 34113367 es_ES
dc.identifier.pmcid PMC8186446 es_ES
dc.relation.pasarela S\438211 es_ES
upv.costeAPC 3420 es_ES


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