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Interferometric multilayered nanomaterials for imaging unlabeled biorecognition events

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Interferometric multilayered nanomaterials for imaging unlabeled biorecognition events

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dc.contributor.author Sancho-Fornes, Gabriel es_ES
dc.contributor.author Avella-Oliver, Miquel es_ES
dc.contributor.author Carrascosa Rubio, Javier es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Maquieira Catala, Angel es_ES
dc.date.accessioned 2022-04-27T06:28:04Z
dc.date.available 2022-04-27T06:28:04Z
dc.date.issued 2021-03-15 es_ES
dc.identifier.issn 0925-4005 es_ES
dc.identifier.uri http://hdl.handle.net/10251/182138
dc.description.abstract [EN] There is an intense scientific activity on nanomaterials for biosensing, supported by their great potential to conceive powerful applications in chemistry, biology, and medicine. In addition to meet the required analytical parameters, the social impact of these advances is also highly influenced by cost, simplicity, and portability aspects, which are critical in label-free systems. This paper addresses the design, development, and experimental assessment of multilayered nanomaterials to transduce unlabeled biorecognition assays as both constructive and destructive interferences, by means of simple and effective imaging setups. The materials rely on superposed nanometric films of metals (gold and Ag-In-Sb-Te alloy) and a dielectric (zinc sulphide) deposited on a polymeric substrate. They are tailored to display maximal (constructive and destructive) interferences for immunoassays performed on their surface, and then fabricated by sputtering and characterized by focused ion beam scanning electron microscopy. Herein we also address the development of a simple optical setup that exploits standard DVD laser units to scan by imaging the reflected interferometric response of microarrayed immunoassays. The bioanalytical performance of the approach is experimentally assessed using a representative model immunoassay (BSA/anti-BSA) and a competitive immunoassay to quantify a low molecular weight drug (sulfasalazine), reaching detection limits of 460 and 11 ng mL(-1) of unlabeled targets, respectively. This study also explores two alternative one-shot interferometric imaging approaches that provide insights into simpler and faster strategies for label-free biosensing. es_ES
dc.description.sponsorship This research was supported by FEDER and the Spanish Ministry of Economy and Competitiveness (CTQ2016-75749-R). The authors thank Angel Lopez Munoz for the construction of the automatized positioning stage. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation info:eu-repo/grantAgreement/AEI//CTQ2016-75749-R//BIOSENSORES HOLOGRAFICOS. PRUEBA DE CONCEPTO Y DEMOSTRACION EN APLICACIONES CLINICAS/ es_ES
dc.relation.ispartof Sensors and Actuators B Chemical es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Interferometry es_ES
dc.subject Nanomaterial es_ES
dc.subject Imaging es_ES
dc.subject Biosensor es_ES
dc.subject Label-free es_ES
dc.subject Sulfasalazine es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Interferometric multilayered nanomaterials for imaging unlabeled biorecognition events es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.snb.2020.129289 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//CTQ2013-45875-R/ES/BIOSENSADO EN SOPORTES INTERACTIVOS CON PROPIEDADES INTERFEROMETRICAS PARA APLICACIONES CLINICAS. DEMOSTRACION EN FARMACOGENETICA Y ALERGIAS MEDICAMENTOSAS/ es_ES
dc.rights.accessRights Embargado es_ES
dc.date.embargoEndDate 2022-12-10 es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Sancho-Fornes, G.; Avella-Oliver, M.; Carrascosa Rubio, J.; Puchades, R.; Maquieira Catala, A. (2021). Interferometric multilayered nanomaterials for imaging unlabeled biorecognition events. Sensors and Actuators B Chemical. 331:1-9. https://doi.org/10.1016/j.snb.2020.129289 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.snb.2020.129289 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 9 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 331 es_ES
dc.relation.pasarela S\455738 es_ES
dc.contributor.funder AGENCIA ESTATAL DE INVESTIGACION es_ES
dc.contributor.funder MINISTERIO DE ECONOMIA Y EMPRESA es_ES
dc.contributor.funder European Regional Development Fund es_ES


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