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A genetic approach reveals different modes of action of prefoldins

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A genetic approach reveals different modes of action of prefoldins

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dc.contributor.author Esteve-Bruna, David es_ES
dc.contributor.author Blanco-Touriñán, Noel es_ES
dc.contributor.author Serrano-Mislata, Antonio es_ES
dc.contributor.author Esquinas-Ariza, Rosa María es_ES
dc.contributor.author Resentini, Francesca es_ES
dc.contributor.author Forment Millet, José Javier es_ES
dc.contributor.author Carrasco-López, Cristian es_ES
dc.contributor.author Novella-Rausell, Claudio es_ES
dc.contributor.author Palacios-Abella, Alberto es_ES
dc.contributor.author Carrasco, Pedro es_ES
dc.contributor.author Salinas, Julio es_ES
dc.contributor.author BLAZQUEZ RODRIGUEZ, MIGUEL ANGEL es_ES
dc.contributor.author ALABADÍ DIEGO, DAVID es_ES
dc.date.accessioned 2022-05-12T18:06:54Z
dc.date.available 2022-05-12T18:06:54Z
dc.date.issued 2021-11 es_ES
dc.identifier.issn 0032-0889 es_ES
dc.identifier.uri http://hdl.handle.net/10251/182576
dc.description.abstract [EN] The prefoldin complex (PFDc) was identified in humans as a co-chaperone of the cytosolic chaperonin T-COMPLEX PROTEIN RING COMPLEX (TRiC)/CHAPERONIN CONTAINING TCP-1 (CCT). PFDc is conserved in eukaryotes and is composed of subunits PFD1-6, and PFDc-TRiC/CCT folds actin and tubulins. PFDs also participate in a wide range of cellular processes, both in the cytoplasm and in the nucleus, and their malfunction causes developmental alterations and disease in animals and altered growth and environmental responses in yeast and plants. Genetic analyses in yeast indicate that not all of their functions require the canonical complex. The lack of systematic genetic analyses in plants and animals, however, makes it difficult to discern whether PFDs participate in a process as the canonical complex or in alternative configurations, which is necessary to understand their mode of action. To tackle this question, and on the premise that the canonical complex cannot be formed if one subunit is missing, we generated an Arabidopsis (Arabidopsis thaliana) mutant deficient in the six PFDs and compared various growth and environmental responses with those of the individual mutants. In this way, we demonstrate that the PFDc is required for seed germination, to delay flowering, or to respond to high salt stress or low temperature, whereas at least two PFDs redundantly attenuate the response to osmotic stress. A coexpression analysis of differentially expressed genes in the sextuple mutant identified several transcription factors, including ABA INSENSITIVE 5 (ABI5) and PHYTOCHROME-INTERACTING FACTOR 4, acting downstream of PFDs. Furthermore, the transcriptomic analysis allowed assigning additional roles for PFDs, for instance, in response to higher temperature. es_ES
dc.description.sponsorship This work was supported by grants from the Spanish Ministry of Economy and Competitiveness and "Agencia Estatal de Investigacion"/FEDER/European Union (BIO2013-43184-P to D.A. and M.A.B., and BIO2016-79133-P and PID2019-109925GB-I00 to D.A.). N.B.-T., A.S.-M., and A.P.-A. were recipient of Ministerio de Economia y Competitividad (BES-2014-068868), EU MSCA-IF (H2020-MSCA-IF-2016746396) and Ministerio de Educacion (FPU17/05186) fellowships, respectively. es_ES
dc.language Inglés es_ES
dc.publisher American Society of Plant Biologists es_ES
dc.relation info:eu-repo/grantAgreement/MINECO//BIO2016-79133-P/ es_ES
dc.relation info:eu-repo/grantAgreement/ME//FPU17%2F05186/ es_ES
dc.relation.ispartof Plant Physiology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.subject.classification GENETICA es_ES
dc.title A genetic approach reveals different modes of action of prefoldins es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/plphys/kiab348 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109925GB-I00/ES/PAPEL DE LAS PREFOLDIN-LIKE EN SEÑALIZACION AMBIENTAL Y HOMEOSTASIS DE PROTEINAS EN ARABIDOPSIS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/746396/EU es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BES-2014-068868/ES/BES-2014-068868/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2013-43184-P/ES/PAPEL DE LA PREFOLDINA EN EL NUCLEO DEPENDIENTE DE DELLAS EN ARABIDOPSIS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Esteve-Bruna, D.; Blanco-Touriñán, N.; Serrano-Mislata, A.; Esquinas-Ariza, RM.; Resentini, F.; Forment Millet, JJ.; Carrasco-López, C.... (2021). A genetic approach reveals different modes of action of prefoldins. Plant Physiology. 187(3):1534-1550. https://doi.org/10.1093/plphys/kiab348 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1093/plphys/kiab348 es_ES
dc.description.upvformatpinicio 1534 es_ES
dc.description.upvformatpfin 1550 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 187 es_ES
dc.description.issue 3 es_ES
dc.identifier.pmid 34618031 es_ES
dc.identifier.pmcid PMC8566299 es_ES
dc.relation.pasarela S\459705 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Ministerio de Educación es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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