Sanchez-Lopez, J.; Atarés Huerta, A.; Jaquez-Gutierrez, M.; Ortiz-Atienza, A.; Capel, C.; Pineda Chaza, BJ.; García Sogo, B.... (2021). Approaching the genetic dissection of indirect adventitious organogenesis
process in tomato explants. Plant Science. 302:1-14. https://doi.org/10.1016/j.plantsci.2020.110721
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/182577
Title:
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Approaching the genetic dissection of indirect adventitious organogenesis
process in tomato explants
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Author:
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Sanchez-Lopez, Jorge
Atarés Huerta, Alejandro
Jaquez-Gutierrez, Marybel
Ortiz-Atienza, Ana
Capel, Carmen
Pineda Chaza, Benito José
García Sogo, Begoña
Yuste-Lisbona, Fernando J.
Lozano, Rafael
Moreno Ferrero, Vicente
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UPV Unit:
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Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
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Issued date:
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Abstract:
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[EN] The screening of 862 T-DNA lines was carried out to approach the genetic dissection of indirect adventitious organogenesis in tomato. Several mutants defective in different phases of adventitious organogenesis, namely ...[+]
[EN] The screening of 862 T-DNA lines was carried out to approach the genetic dissection of indirect adventitious organogenesis in tomato. Several mutants defective in different phases of adventitious organogenesis, namely callus growth (tdc-1), bud differentiation (tdb-1,-2,-3) and shoot-bud development (tds-1) were identified and characterized. The alteration of the TDC-1 gene blocked callus proliferation depending on the composition of growth regulators in the culture medium. Calli from tds-1 explants differentiated buds but did not develop normal shoots. Histological analysis showed that their abnormal development is due to failure in the organization of normal adventitious shoot meristems. Interestingly, tdc-1 and tds-1 mutant plants were indistinguishable from WT ones, indicating that the respective altered genes play specific roles in cell proliferation from explant cut zones (TDC-1 gene) or in the organization of adventitious shoot meristems (TDS-1 gene). Unlike the previous, plants of the three mutants defective in the differentiation of adventitious shoot-buds (tdb-1,-2,-3) showed multiple changes in vegetative and reproductive traits. Cosegregation analyses revealed the existence of an association between the phenotype of the tdb-3 mutant and a T-DNA insert, which led to the discovery that the SlMAPKKK17 gene is involved in the shoot-bud differentiation process.
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Subjects:
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Tomato
,
Mutants
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Adventitious organogenesis
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Shoot-bud differentiation
,
SlMAPKKK17
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Copyrigths:
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Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
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Source:
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Plant Science. (issn:
0168-9452
)
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DOI:
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10.1016/j.plantsci.2020.110721
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Publisher:
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Elsevier
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Publisher version:
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https://doi.org/10.1016/j.plantsci.2020.110721
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Project ID:
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110833RB-C31/ES/REGULACION GENETICA DE LA ACTIVIDAD DE LOS MERISTEMOS REPRODUCTIVOS Y SU PAPEL EN LA MEJORA DE LA PRODUCTIVIDAD DE TOMATE/
info:eu-repo/grantAgreement/UPV//PAID-06-18/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110833RB-C32/ES/EDICION DE PROMOTORES DE GENES QUE REGULAN EL DESARROLLO REPRODUCTIVO EN TOMATE COMO ESTRATEGIA PARA MANTENER LA PRODUCCION EN CONDICIONES DE ESTRES ABIOTICO/
info:eu-repo/grantAgreement/UPV-VIN//SP20180181//Caracterización del papel funcional del gen RAMLESS en el desarrollo radicular de tomate y la
tolerancia a estrés abiótico/
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Thanks:
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Vicente Moreno and Rafael Lozano thank the Ministry of Science and Innovation (State Innovation Agency) for granting the projects PID2019-110833RB-C32 and PID2019-110833RB-C31. Benito Pineda's work in the context of this ...[+]
Vicente Moreno and Rafael Lozano thank the Ministry of Science and Innovation (State Innovation Agency) for granting the projects PID2019-110833RB-C32 and PID2019-110833RB-C31. Benito Pineda's work in the context of this article has been funded by 'Aid for First Research Projects (PAID-06-18)' by the Vicerrectorado de Investigacion, Innovacion y Transferencia de la Universitat Politecnica de Valencia (UPV), Valencia, Spain'. The PhD fellowship for Jorge Sanchez-Lopez and Marybel Jaquez-Gutierrez were funded by the Universidad de Sinaloa and the CONACYT of Mexico.
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Type:
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Artículo
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