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Preparation of monolithic polymer-magnetite nanoparticle composites into poly(ethylene-co-tetrafluoroethylene) tubes for uses in micro-bore HPLC separation and extraction of phosphorylated compounds

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Preparation of monolithic polymer-magnetite nanoparticle composites into poly(ethylene-co-tetrafluoroethylene) tubes for uses in micro-bore HPLC separation and extraction of phosphorylated compounds

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dc.contributor.author Torres-Cartas, Sagrario es_ES
dc.contributor.author Meseguer-Lloret, S. es_ES
dc.contributor.author Gómez Benito, Carmen es_ES
dc.contributor.author Catalá-Icardo, Mónica es_ES
dc.contributor.author Simo-Alfonso, Ernesto F. es_ES
dc.contributor.author Herrero-Martínez, José Manuel es_ES
dc.date.accessioned 2022-06-06T18:02:44Z
dc.date.available 2022-06-06T18:02:44Z
dc.date.issued 2021-03-01 es_ES
dc.identifier.issn 0039-9140 es_ES
dc.identifier.uri http://hdl.handle.net/10251/183098
dc.description.abstract [EN] This paper describes the fabrication of a novel microbore monolithic column modified with magnetite nanoparticles (MNPs) prepared in a poly(ethylene-co-tetrafluoroethylene) (EFTE) tubing, and its application as stationary phase for the chromatographic separation of phosphorylated compounds. In order to obtain the composite column, a two-step procedure was performed. The formation of a glycidyl methacrylate-based monolith inside the activated ETFE tube was firstly carried out. Then, two incorporation approaches of MNPs in monoliths were investigated. The generic polymer was modified with 3-aminopropyltrimethoxysilane (APTMS) to be subsequently attached to MNP surfaces. Alternatively, APTMS-coated MNPs were firstly prepared and subsequently used for attachment onto the monolith surface through reaction of epoxy groups present in the generic monolith. This last strategy gave a reproducible layer of MNPs coated onto the polymer monolith as well as robust and permeable chromatographic columns. The retention behaviour of this MNP-based composite monolithic column was studied by using small phosphorylated compounds (adenosine phosphates). It was found that the retention of model analytes was ruled by partitioning and adsorption HILIC mechanisms. The columns also exhibited satisfactory performance in the separation of these target compounds, showing good chromatographic behaviour after two months of continued use. These composite monolithic columns were also successfully applied to the extraction of a tryptic digest of beta-casein. es_ES
dc.description.sponsorship This work was supported by project RTI2018-095536-B-I00 (Ministry of Science, Innovation and Universities, Spain). The proteomic analysis was performed in the proteomics facility of SCSIE (University of Valencia) that belongs to ProteoRed, PRB2-ISCIII, supported by grant PT13/0001 (Instituto de Carlos III, Spain). The authors also thank Dr. Luz Valero-Rustarazo for her assistance in proteomic analysis and data treatment es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation info:eu-repo/grantAgreement/UV//PT13%2F0001/ es_ES
dc.relation.ispartof Talanta es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Monolithic column es_ES
dc.subject Magnetite nanoparticles es_ES
dc.subject Hybrid monoliths es_ES
dc.subject Adenosine phosphates es_ES
dc.subject EFTE tubing es_ES
dc.subject HILIC es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Preparation of monolithic polymer-magnetite nanoparticle composites into poly(ethylene-co-tetrafluoroethylene) tubes for uses in micro-bore HPLC separation and extraction of phosphorylated compounds es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.talanta.2020.121806 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095536-B-I00/ES/DESARROLLO DE MATERIALES POLIMERICOS FUNCIONALES EN PLATAFORMAS FLEXIBLES PARA APLICACIONES MEDIOAMBIENTALES Y TOXICOLOGICAS/ es_ES
dc.rights.accessRights Embargado es_ES
dc.date.embargoEndDate 2023-03-01 es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Torres-Cartas, S.; Meseguer-Lloret, S.; Gómez Benito, C.; Catalá-Icardo, M.; Simo-Alfonso, EF.; Herrero-Martínez, JM. (2021). Preparation of monolithic polymer-magnetite nanoparticle composites into poly(ethylene-co-tetrafluoroethylene) tubes for uses in micro-bore HPLC separation and extraction of phosphorylated compounds. Talanta. 224:121806-121813. https://doi.org/10.1016/j.talanta.2020.121806 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.talanta.2020.121806 es_ES
dc.description.upvformatpinicio 121806 es_ES
dc.description.upvformatpfin 121813 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 224 es_ES
dc.identifier.pmid 33379032 es_ES
dc.relation.pasarela S\422303 es_ES
dc.contributor.funder Universitat de València es_ES
dc.contributor.funder MINISTERIO DE CIENCIA, INNOVACIÓN Y UNIVERSIDADES es_ES


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