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Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector

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Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector

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Uranga-Ruiz De Eguino, M.; Aragones, V.; Selma García, S.; Vázquez-Vilar, M.; Orzáez Calatayud, DV.; Daròs, J. (2021). Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector. The Plant Journal. 106(2):555-565. https://doi.org/10.1111/tpj.15164

Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/183588

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Title: Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector
Author: Uranga-Ruiz De Eguino, Mireia Aragones, V Selma García, Sara Vázquez-Vilar, Marta Orzáez Calatayud, Diego Vicente Daròs, José-Antonio
UPV Unit: Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
Issued date:
Abstract:
[EN] Systems based on the clustered, regularly interspaced, short palindromic repeat (CRISPR) and CRISPR-associated proteins (Cas) have revolutionized genome editing in many organisms, including plants. Most CRISPR-Cas ...[+]
Subjects: CRISPR-Cas9 , SgRNA expression , Virus-induced genome editing , Multiplexing , Potato virus X , Solanaceous plants
Copyrigths: Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
Source:
The Plant Journal. (issn: 0960-7412 )
DOI: 10.1111/tpj.15164
Publisher:
Blackwell Publishing
Publisher version: https://doi.org/10.1111/tpj.15164
Project ID:
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83184-R/ES/VIRUS DE PLANTAS: PATOGENOS Y TAMBIEN VECTORES PARA LA PRODUCCION DE PROTEINAS, METABOLITOS, RNAS Y NANOPARTICULAS/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108203RB-I00/ES/EXPLOITING THE MODULAR ARCHITECTURE OF CRISPR%2FCAS TO DESIGN PROGRAMMABLE GENE CIRCUITS AND OTHER NEW BREEDING TOOLS IN PLANTS/
info:eu-repo/grantAgreement/EC/H2020/760331/EU
info:eu-repo/grantAgreement/MICINN//BES-2017-0890098/
info:eu-repo/grantAgreement/MICINN//FPU17%2F05503/
Thanks:
This work was supported by grants BIO2017-83184-R and PID2019-108203RB-I00 from Ministerio de Ciencia e Innovacion (Spain) through the Agencia Estatal de Investigacion (co-financed European Regional Development Fund), and ...[+]
Type: Artículo

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