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Screening of Suitable Plant Regeneration Protocols for Several Capsicum spp. through Direct Organogenesis

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Screening of Suitable Plant Regeneration Protocols for Several Capsicum spp. through Direct Organogenesis

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dc.contributor.author Martínez-López, Marina es_ES
dc.contributor.author García-Pérez, Ana es_ES
dc.contributor.author Gimeno -Páez, Esther es_ES
dc.contributor.author Prohens Tomás, Jaime es_ES
dc.contributor.author Vilanova Navarro, Santiago es_ES
dc.contributor.author García-Fortea, Edgar es_ES
dc.date.accessioned 2022-06-30T18:07:42Z
dc.date.available 2022-06-30T18:07:42Z
dc.date.issued 2021-09 es_ES
dc.identifier.uri http://hdl.handle.net/10251/183720
dc.description.abstract [EN] Peppers (Capsicum spp.) are recalcitrant to in vitro culture regeneration, making the application of in vitro-based breeding strategies difficult. We evaluated the impact of different combinations of auxins, cytokinins and micronutrients on the induction of direct organogenesis in cotyledon and hypocotyl explants of C. annuum, C. baccatum and C. chinense. We found variation in the regeneration response among species and type of explant. In this way, the average numbers of shoots per cotyledon and hypocotyl explant were, respectively, 1.44 and 0.28 for C. annuum, 4.17 and 3.20 for C. baccatum and 0.08 and 0.00 for C. chinense. Out of the six media, the best overall results were obtained with the medium Pep1, which contained 5 mg/L BAP (6-benzylaminopurine), 0.5 mg/L IAA (indole-3-acetic acid) and 0.47 mg/L CuSO4, followed by a subculture in the same medium supplemented with 10 mg/L AgNO3 (medium Pep1.2). The best result for the Pep1 + Pep1.2 medium was obtained for C. baccatum using cotyledon explants, with 8.87 shoots per explant. The explants grown in medium Pep1 + Pep1.2 were the ones with greener tissue, while overall the hypocotyl explants were greener than the cotyledon explants. Our results indicate that there is wide variation among Capsicum species in terms of regeneration. Our results suggest that the synergistic effect of copper and silver resulted in a higher regeneration rate of Capsicum explants. Explants with shoots were transferred to different media for elongation, rooting and acclimatization. Although acclimatized plantlets were obtained for C. baccatum and C. chinense, an improvement in these latter stages would be desirable for a high throughput regeneration pipeline. This work contributes to the improvement of Capsicum regeneration protocols using specific combinations of medium, explant and genotype, reaching the levels of efficiency required for genetic transformation and of gene editing technologies for other crops. es_ES
dc.description.sponsorship This research was funded by Universitat Politecnica de Valencia, research line 20200268. es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Horticulturae es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Direct organogenesis es_ES
dc.subject AgNO3 es_ES
dc.subject CuSO4 es_ES
dc.subject Capsicum annuum es_ES
dc.subject Capsicum baccatum es_ES
dc.subject Capsicum chinense es_ES
dc.subject.classification GENETICA es_ES
dc.title Screening of Suitable Plant Regeneration Protocols for Several Capsicum spp. through Direct Organogenesis es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/horticulturae7090261 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//20200268/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Martínez-López, M.; García-Pérez, A.; Gimeno -Páez, E.; Prohens Tomás, J.; Vilanova Navarro, S.; García-Fortea, E. (2021). Screening of Suitable Plant Regeneration Protocols for Several Capsicum spp. through Direct Organogenesis. Horticulturae. 7(9):1-17. https://doi.org/10.3390/horticulturae7090261 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/horticulturae7090261 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 17 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.description.issue 9 es_ES
dc.identifier.eissn 2311-7524 es_ES
dc.relation.pasarela S\461413 es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
dc.subject.ods 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible es_ES
upv.costeAPC 1694 es_ES


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