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Reconstruction of the absorption spectrum of Synechocystis sp. PCC 6803 optical mutants from the in vivo signature of individual pigments

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Reconstruction of the absorption spectrum of Synechocystis sp. PCC 6803 optical mutants from the in vivo signature of individual pigments

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dc.contributor.author Fuente Herraiz, David es_ES
dc.contributor.author Lazar, Dusan es_ES
dc.contributor.author Oliver Villanueva, José Vicente es_ES
dc.contributor.author Urchueguía Schölzel, Javier Fermín es_ES
dc.date.accessioned 2022-06-30T18:08:08Z
dc.date.available 2022-06-30T18:08:08Z
dc.date.issued 2021-01 es_ES
dc.identifier.issn 0166-8595 es_ES
dc.identifier.uri http://hdl.handle.net/10251/183735
dc.description.abstract [EN] In this work, we reconstructed the absorption spectrum of different Synechocystis sp. PCC 6803 optical strains by summing the computed signature of all pigments present in this organism. To do so, modifications to in vitro pigment spectra were first required: namely wavelength shift, curve smoothing, and the package effect calculation derived from high pigment densities were applied. As a result, we outlined a plausible shape for the in vivo absorption spectrum of each chromophore. These are flatter and slightly broader in physiological conditions yet the mean weight-specific absorption coefficient remains identical to the in vitro conditions. Moreover, we give an estimate of all pigment concentrations without applying spectrophotometric correlations, which are often prone to error. The computed cell spectrum reproduces in an accurate manner the experimental spectrum for all the studied wavelengths in the wild-type, Olive, and PAL strain. The gathered pigment concentrations are in agreement with reported values in literature. Moreover, different illumination set-ups were evaluated to calculate the mean absorption cross-section of each chromophore. Finally, a qualitative estimate of light-limited cellular growth at each wavelength is given. This investigation describes a novel way to approach the cell absorption spectrum and shows all its inherent potential for photosynthesis research. es_ES
dc.description.sponsorship DF was supported by an internal grant of Palacky University Olomouc (no. IGA_ PrF_2020_028) and DL by the ERDF project "Plants as a tool for sustainable global development" (no. CZ.02.1.01/0.0/0.0/16-019/0000827)." es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof Photosynthesis Research es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Absorption es_ES
dc.subject Spectrum es_ES
dc.subject Light es_ES
dc.subject Pigment es_ES
dc.subject Modeling es_ES
dc.subject Synechocystis es_ES
dc.subject Photosystem es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.subject.classification INGENIERIA AGROFORESTAL es_ES
dc.title Reconstruction of the absorption spectrum of Synechocystis sp. PCC 6803 optical mutants from the in vivo signature of individual pigments es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s11120-020-00799-8 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Palacký University Olomouc//IGA_ PrF_2020_028/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FEDER//CZ.02.1.01%2F0.0%2F0.0%2F16-019%2F0000827/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Rural y Agroalimentaria - Departament d'Enginyeria Rural i Agroalimentària es_ES
dc.description.bibliographicCitation Fuente Herraiz, D.; Lazar, D.; Oliver Villanueva, JV.; Urchueguía Schölzel, JF. (2021). Reconstruction of the absorption spectrum of Synechocystis sp. PCC 6803 optical mutants from the in vivo signature of individual pigments. Photosynthesis Research. 147(1):75-90. https://doi.org/10.1007/s11120-020-00799-8 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s11120-020-00799-8 es_ES
dc.description.upvformatpinicio 75 es_ES
dc.description.upvformatpfin 90 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 147 es_ES
dc.description.issue 1 es_ES
dc.identifier.pmid 33245462 es_ES
dc.relation.pasarela S\445885 es_ES
dc.contributor.funder Palacký University Olomouc es_ES
dc.contributor.funder European Regional Development Fund es_ES
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