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Genetic diversity and virulence gene profiles of Escherichia coli from diarrhoeal rabbits in Sichuan Province, China

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Genetic diversity and virulence gene profiles of Escherichia coli from diarrhoeal rabbits in Sichuan Province, China

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dc.contributor.author Yang, Yuqing es_ES
dc.contributor.author Wang, Shizhen es_ES
dc.contributor.author Geng, Yi es_ES
dc.contributor.author Liu, Hanzhong es_ES
dc.contributor.author Qin, Zhenyang es_ES
dc.contributor.author Feng, Yang es_ES
dc.contributor.author Yang, Zexiao es_ES
dc.contributor.author Lai, Weimin es_ES
dc.coverage.spatial east=104.07572; north=30.6508899; name=Sichuan, 督院街30号 邮政编码: 610093 es_ES
dc.date.accessioned 2022-07-07T12:01:56Z
dc.date.available 2022-07-07T12:01:56Z
dc.date.issued 2022-06-29
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/183938
dc.description.abstract [EN] Escherichia coli (E. coli) can cause diarrhoea in a wide range of hosts. Moreover, some trains with high virulence and drug resistance pose a serious threat to public health and livestock products. Diarrhoea caused by E. coli outbreaks in rabbitries result in serious economic losses. The aim of this study was to investigate the distribution of virulence genes and molecular genetic characteristics of E. coli from diarrhoeal rabbits in the main rearing areas of Sichuan province, China in 2015-2017. In total, 39 E. coli isolates were identified and undivided divided into 17 sequence types by multilocus sequence typing (MLST) and grouped in 22 clusters by pulsed-field gel electrophoresis. Polymerase chain reaction tests detected 6 virulence genes: eae (41.0%), ler (41.0%), ral (33.3%), afr2 (10.3%), irp2 (15.4%) and astA (7.7%) of the tested 17 virulence genes identifying 16 enteropathogenic E. coli (EPEC) isolates. The main sequence types U328, ST328 and ST20 carried rabbit EPEC associated virulence genes (eae, ler, ral and afr2). The results showed that the distribution of virulence genes varied by year and area; genotype had major types in local rearing areas but was of high diversity in Sichuan province. es_ES
dc.description.sponsorship Sichuan Technology Support Planning (2016NZ0002) es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València es_ES
dc.relation.ispartof World Rabbit Science es_ES
dc.rights Reconocimiento - No comercial - Compartir igual (by-nc-sa) es_ES
dc.subject Escherichia coli es_ES
dc.subject Genotyping es_ES
dc.subject Rabbit es_ES
dc.subject Virulence gene profiles es_ES
dc.title Genetic diversity and virulence gene profiles of Escherichia coli from diarrhoeal rabbits in Sichuan Province, China es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.4995/wrs.2022.15273
dc.relation.projectID info:eu-repo/grantAgreement/Sichuan Technology Support Planning//2016NZ0002 es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Yang, Y.; Wang, S.; Geng, Y.; Liu, H.; Qin, Z.; Feng, Y.; Yang, Z.... (2022). Genetic diversity and virulence gene profiles of Escherichia coli from diarrhoeal rabbits in Sichuan Province, China. World Rabbit Science. 30(2):131-140. https://doi.org/10.4995/wrs.2022.15273 es_ES
dc.description.accrualMethod OJS es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2022.15273 es_ES
dc.description.upvformatpinicio 131 es_ES
dc.description.upvformatpfin 140 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 30 es_ES
dc.description.issue 2 es_ES
dc.identifier.eissn 1989-8886
dc.relation.pasarela OJS\15273 es_ES
dc.contributor.funder Sichuan Province Science and Technology Support Program es_ES
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