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dc.contributor.author | Estébanez, Sandra | es_ES |
dc.contributor.author | Rivera, Ana M. | es_ES |
dc.contributor.author | Recky, Jael R. Neyra | es_ES |
dc.contributor.author | Thomas, Andres H. | es_ES |
dc.contributor.author | Lhiaubet, Virginie Lyria | es_ES |
dc.contributor.author | Lorente, Carolina | es_ES |
dc.date.accessioned | 2022-07-18T18:05:26Z | |
dc.date.available | 2022-07-18T18:05:26Z | |
dc.date.issued | 2021-10 | es_ES |
dc.identifier.issn | 0891-5849 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/184366 | |
dc.description.abstract | [EN] Pterin (Ptr) is a model photosensitizer that acts mainly through type I mechanism and is able to photoinduce the one-electron oxidation of purine and pyrimidine nucleobases. However, under anaerobic conditions Ptr reacts with thymine (T) to form photoadducts (Ptr-T) but does not lead to the photodegradation of guanine (G), which is the nucleobase with the lowest ionization potential. Accordingly, G is thermodynamically able to reduce the radicals of the other nucleobases and has been described in this sense as the "hole sink" of the DNA double helix. Here we analyze by steady-state and time-resolved studies the effect of G in the anaerobic photosensitization of T by Ptr, using nucleotides and oligonucleotides of different sequences. We demonstrated that G is able to reduce T radicals but does not prevent the formation of Ptr-T adducts. Our results suggest that after the encounter between the excited Ptr and T, and completion of the electron transfer step, part of the radicals escape from the solvent cage, to further react with other species. However, a proportion of radicals do not escape and evolve to photoadducts before separation. We provide new evidence that contributes to understand the photosensitizing properties of Ptr in the absence of O-2, the mechanism of formation of photoadducts in the DNA and the protective role of G towards the photodamage in other nucleobases. | es_ES |
dc.description.sponsorship | The present work was partially supported by Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET-Grant P-UE 2017 22920170100100CO) , Agencia de Promocion Cientifica y Tecnologica (ANPCyT-Grant PICT 2015-1988, and PICT 2017-0925) , Universidad Nacional de La Plata (UNLP-Grant 11/X840) . Funding from the Program CSIC de Cooperacion Cientifica para el Desarrollo (iCOOP-Light project ref 20105CD0017) and Spanish Government (PGC2018-096684-B-I00) are gratefully acknowledged. S.E.R. and J.R.N.R. thanks ANPCyT and CONICET for doctoral research fellowships. A.M.R. thanks Universidad de Antioquia (Colombia) for research fellowship. C.L. and A.H.T. are research members of CONICET. V.L.V. is research member of CSIC. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Free Radical Biology and Medicine | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Electron-transfer | es_ES |
dc.subject | Photosensitization | es_ES |
dc.subject | Nuclebases | es_ES |
dc.subject | Pterin | es_ES |
dc.subject.classification | QUIMICA ORGANICA | es_ES |
dc.title | Pterin-photosensitization of thymine under anaerobic conditions in the presence of guanine | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.freeradbiomed.2021.07.032 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-096684-B-I00/ES/REPARACION DEL ADN POR PROCESOS MULTIFOTONICOS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/CONICET//P-UE 2017 22920170100100CO/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ANPCyT//PICT 2015-1988/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ANPCyT//PICT 2017-0925/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/UNLP//11%2FX840/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/CSIC//20105CD0017//iCOOPLight project/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Tecnología Química - Institut Universitari Mixt de Tecnologia Química | es_ES |
dc.description.bibliographicCitation | Estébanez, S.; Rivera, AM.; Recky, JRN.; Thomas, AH.; Lhiaubet, VL.; Lorente, C. (2021). Pterin-photosensitization of thymine under anaerobic conditions in the presence of guanine. Free Radical Biology and Medicine. 174:321-328. https://doi.org/10.1016/j.freeradbiomed.2021.07.032 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.freeradbiomed.2021.07.032 | es_ES |
dc.description.upvformatpinicio | 321 | es_ES |
dc.description.upvformatpfin | 328 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 174 | es_ES |
dc.identifier.pmid | 34339797 | es_ES |
dc.relation.pasarela | S\461465 | es_ES |
dc.contributor.funder | Universidad Nacional de La Plata, Argentina | es_ES |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas | es_ES |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades | es_ES |
dc.contributor.funder | Agencia Nacional de Promoción Científica y Tecnológica, Argentina | es_ES |
dc.contributor.funder | Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina | es_ES |