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Pterin-photosensitization of thymine under anaerobic conditions in the presence of guanine

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Pterin-photosensitization of thymine under anaerobic conditions in the presence of guanine

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dc.contributor.author Estébanez, Sandra es_ES
dc.contributor.author Rivera, Ana M. es_ES
dc.contributor.author Recky, Jael R. Neyra es_ES
dc.contributor.author Thomas, Andres H. es_ES
dc.contributor.author Lhiaubet, Virginie Lyria es_ES
dc.contributor.author Lorente, Carolina es_ES
dc.date.accessioned 2022-07-18T18:05:26Z
dc.date.available 2022-07-18T18:05:26Z
dc.date.issued 2021-10 es_ES
dc.identifier.issn 0891-5849 es_ES
dc.identifier.uri http://hdl.handle.net/10251/184366
dc.description.abstract [EN] Pterin (Ptr) is a model photosensitizer that acts mainly through type I mechanism and is able to photoinduce the one-electron oxidation of purine and pyrimidine nucleobases. However, under anaerobic conditions Ptr reacts with thymine (T) to form photoadducts (Ptr-T) but does not lead to the photodegradation of guanine (G), which is the nucleobase with the lowest ionization potential. Accordingly, G is thermodynamically able to reduce the radicals of the other nucleobases and has been described in this sense as the "hole sink" of the DNA double helix. Here we analyze by steady-state and time-resolved studies the effect of G in the anaerobic photosensitization of T by Ptr, using nucleotides and oligonucleotides of different sequences. We demonstrated that G is able to reduce T radicals but does not prevent the formation of Ptr-T adducts. Our results suggest that after the encounter between the excited Ptr and T, and completion of the electron transfer step, part of the radicals escape from the solvent cage, to further react with other species. However, a proportion of radicals do not escape and evolve to photoadducts before separation. We provide new evidence that contributes to understand the photosensitizing properties of Ptr in the absence of O-2, the mechanism of formation of photoadducts in the DNA and the protective role of G towards the photodamage in other nucleobases. es_ES
dc.description.sponsorship The present work was partially supported by Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET-Grant P-UE 2017 22920170100100CO) , Agencia de Promocion Cientifica y Tecnologica (ANPCyT-Grant PICT 2015-1988, and PICT 2017-0925) , Universidad Nacional de La Plata (UNLP-Grant 11/X840) . Funding from the Program CSIC de Cooperacion Cientifica para el Desarrollo (iCOOP-Light project ref 20105CD0017) and Spanish Government (PGC2018-096684-B-I00) are gratefully acknowledged. S.E.R. and J.R.N.R. thanks ANPCyT and CONICET for doctoral research fellowships. A.M.R. thanks Universidad de Antioquia (Colombia) for research fellowship. C.L. and A.H.T. are research members of CONICET. V.L.V. is research member of CSIC. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Free Radical Biology and Medicine es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Electron-transfer es_ES
dc.subject Photosensitization es_ES
dc.subject Nuclebases es_ES
dc.subject Pterin es_ES
dc.subject.classification QUIMICA ORGANICA es_ES
dc.title Pterin-photosensitization of thymine under anaerobic conditions in the presence of guanine es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.freeradbiomed.2021.07.032 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-096684-B-I00/ES/REPARACION DEL ADN POR PROCESOS MULTIFOTONICOS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/CONICET//P-UE 2017 22920170100100CO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ANPCyT//PICT 2015-1988/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ANPCyT//PICT 2017-0925/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UNLP//11%2FX840/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/CSIC//20105CD0017//iCOOPLight project/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Tecnología Química - Institut Universitari Mixt de Tecnologia Química es_ES
dc.description.bibliographicCitation Estébanez, S.; Rivera, AM.; Recky, JRN.; Thomas, AH.; Lhiaubet, VL.; Lorente, C. (2021). Pterin-photosensitization of thymine under anaerobic conditions in the presence of guanine. Free Radical Biology and Medicine. 174:321-328. https://doi.org/10.1016/j.freeradbiomed.2021.07.032 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.freeradbiomed.2021.07.032 es_ES
dc.description.upvformatpinicio 321 es_ES
dc.description.upvformatpfin 328 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 174 es_ES
dc.identifier.pmid 34339797 es_ES
dc.relation.pasarela S\461465 es_ES
dc.contributor.funder Universidad Nacional de La Plata, Argentina es_ES
dc.contributor.funder Consejo Superior de Investigaciones Científicas es_ES
dc.contributor.funder Ministerio de Ciencia, Innovación y Universidades es_ES
dc.contributor.funder Agencia Nacional de Promoción Científica y Tecnológica, Argentina es_ES
dc.contributor.funder Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina es_ES


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