dc.contributor.author |
García-Domínguez, Ximo
|
es_ES |
dc.contributor.author |
Diretto, Gianfranco
|
es_ES |
dc.contributor.author |
Peñaranda, D.S.
|
es_ES |
dc.contributor.author |
Frusciante, Sarah
|
es_ES |
dc.contributor.author |
García-Carpintero, Víctor
|
es_ES |
dc.contributor.author |
Cañizares Sales, Joaquín
|
es_ES |
dc.contributor.author |
Vicente Antón, José Salvador
|
es_ES |
dc.contributor.author |
Marco-Jiménez, Francisco
|
es_ES |
dc.date.accessioned |
2022-09-16T18:04:14Z |
|
dc.date.available |
2022-09-16T18:04:14Z |
|
dc.date.issued |
2021-09 |
es_ES |
dc.identifier.uri |
http://hdl.handle.net/10251/186218 |
|
dc.description.abstract |
[EN] Embryo manipulation is a requisite step in assisted reproductive technology (ART). Therefore, it is of great necessity to appraise the safety of ART and investigate the long-term effect, including lipid metabolism, on ART-conceived offspring. Augmenting our ART rabbit model to investigate lipid metabolic outcomes in offspring longitudinally, we detected variations in hepatic DNA methylation ART offspring in the F3 generation for embryonic exposure (multiple ovulation, vitrification and embryo transfer). Through adult liver metabolomics and proteomics, we identified changes mainly related to lipid metabolism (e.g., polyunsaturated fatty acids, steroids, steroid hormone). We also found that DNA methylation analysis was linked to changes in lipid metabolism and apoptosis genes. Nevertheless, these differences did not apparently alter the general health status. Thus, our findings suggest that ART is likely to be a player in embryo epigenetic events related to hepatic homeostasis alteration in adulthood. |
es_ES |
dc.description.sponsorship |
This research was funded by the Spanish Ministry of Economy and Competitiveness (MINECO), Spain, grant number AGL2014-53405-C2-1-P and by Conselleria d'Educacio, Investigacio, Cultura i Esport, Spain, grant number Prometeo II 2014/036. Ximo Garcia-Dominguez was supported by a research grant from the Ministry of Economy, Industry and Competitiveness of Spain (BES-2015-072429). |
es_ES |
dc.language |
Inglés |
es_ES |
dc.publisher |
MDPI AG |
es_ES |
dc.relation.ispartof |
International Journal of Molecular Sciences |
es_ES |
dc.rights |
Reconocimiento (by) |
es_ES |
dc.subject |
Assisted reproductive technologies |
es_ES |
dc.subject |
Vitrification |
es_ES |
dc.subject |
Stress |
es_ES |
dc.subject |
DNA methylation |
es_ES |
dc.subject |
Metabolome |
es_ES |
dc.subject |
Proteome |
es_ES |
dc.subject |
Steroid biosynthesis |
es_ES |
dc.subject.classification |
GENETICA |
es_ES |
dc.subject.classification |
PRODUCCION ANIMAL |
es_ES |
dc.subject.classification |
BIOLOGIA ANIMAL |
es_ES |
dc.title |
Early Embryo Exposure to Assisted Reproductive Manipulation Induced Subtle Changes in Liver Epigenetics with No Apparent Negative Health Consequences in Rabbit |
es_ES |
dc.type |
Artículo |
es_ES |
dc.identifier.doi |
10.3390/ijms22189716 |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/MINECO//AGL2014-53405-C2-1-P/ES/MEJORA GENETICA DEL CONEJO DE CARNE:RESPUESTA A LA SELECCION Y SU EFECTO SOBRE LA REPRODUCCION, ALIMENTACION Y SALUD UTILIZANDO UNA POBLACION CONTROL CRIOCONSERVADA/ |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/GENERALITAT VALENCIANA//PROMETEOII%2F2014%2F036//Crioconservación de óvulos y embriones. Cambios epigenéticos y repercusiones transcripcionales y proteómicas / |
es_ES |
dc.relation.projectID |
info:eu-repo/grantAgreement/MINECO//BES-2015-072429/ES/BES-2015-072429/ |
es_ES |
dc.rights.accessRights |
Abierto |
es_ES |
dc.contributor.affiliation |
Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal |
es_ES |
dc.contributor.affiliation |
Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia |
es_ES |
dc.description.bibliographicCitation |
García-Domínguez, X.; Diretto, G.; Peñaranda, D.; Frusciante, S.; García-Carpintero, V.; Cañizares Sales, J.; Vicente Antón, JS.... (2021). Early Embryo Exposure to Assisted Reproductive Manipulation Induced Subtle Changes in Liver Epigenetics with No Apparent Negative Health Consequences in Rabbit. International Journal of Molecular Sciences. 22(18):1-17. https://doi.org/10.3390/ijms22189716 |
es_ES |
dc.description.accrualMethod |
S |
es_ES |
dc.relation.publisherversion |
https://doi.org/10.3390/ijms22189716 |
es_ES |
dc.description.upvformatpinicio |
1 |
es_ES |
dc.description.upvformatpfin |
17 |
es_ES |
dc.type.version |
info:eu-repo/semantics/publishedVersion |
es_ES |
dc.description.volume |
22 |
es_ES |
dc.description.issue |
18 |
es_ES |
dc.identifier.eissn |
1422-0067 |
es_ES |
dc.identifier.pmid |
34575877 |
es_ES |
dc.identifier.pmcid |
PMC8467347 |
es_ES |
dc.relation.pasarela |
S\445836 |
es_ES |
dc.contributor.funder |
GENERALITAT VALENCIANA |
es_ES |
dc.contributor.funder |
MINISTERIO DE ECONOMIA Y EMPRESA |
es_ES |
dc.contributor.funder |
Universitat Politècnica de València |
|
upv.costeAPC |
1800 |
es_ES |