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K-seq, an affordable, reliable, and open Klenow NGS-based genotyping technology

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K-seq, an affordable, reliable, and open Klenow NGS-based genotyping technology

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Ziarsolo, P.; Hasing, T.; Hilario, R.; García-Carpintero, V.; Blanca Postigo, JM.; Bombarely, A.; Cañizares Sales, J. (2021). K-seq, an affordable, reliable, and open Klenow NGS-based genotyping technology. Plant Methods. 17(1):1-11. https://doi.org/10.1186/s13007-021-00733-6

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Título: K-seq, an affordable, reliable, and open Klenow NGS-based genotyping technology
Autor: Ziarsolo, Peio Hasing, Tomas Hilario, Rebeca García-Carpintero, Víctor Blanca Postigo, José Miguel Bombarely, Aureliano Cañizares Sales, Joaquín
Entidad UPV: Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana
Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
Fecha difusión:
Resumen:
[EN] Background: K-seq, a new genotyping methodology based on the amplification of genomic regions using two steps of Klenow amplification with short oligonucleotides, followed by standard PCR and Illumina sequencing, is ...[+]
Palabras clave: Klenow polymerase , K-seq , K-mer , Low cost , SNP , Genotyping , GRR , NGS
Derechos de uso: Reconocimiento (by)
Fuente:
Plant Methods. (issn: 1746-4811 )
DOI: 10.1186/s13007-021-00733-6
Editorial:
Springer (Biomed Central Ltd.)
Versión del editor: https://doi.org/10.1186/s13007-021-00733-6
Código del Proyecto:
info:eu-repo/grantAgreement/UPV//20180051//Desarrollo de herramientas para la identificación de genes y loci de interés en la mejora genética del tomate y otras hortícolas/
Agradecimientos:
This work was supported by the University Polytechnic of Valencia, Grant Number 20180051 "Desarrollo de herramientas para la identificacion de genes y loci de interes en la mejora genetica del tomate y otras horticolas".
Tipo: Artículo

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