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The ABCF3 Gene of Arabidopsis Is Functionally Linked with GCN1 but Not with GCN2 During Stress and Development

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The ABCF3 Gene of Arabidopsis Is Functionally Linked with GCN1 but Not with GCN2 During Stress and Development

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dc.contributor.author Faus, I. es_ES
dc.contributor.author Niñoles Rodenes, Regina es_ES
dc.contributor.author Kesari, V. es_ES
dc.contributor.author Gadea Vacas, José es_ES
dc.date.accessioned 2022-09-30T18:06:57Z
dc.date.available 2022-09-30T18:06:57Z
dc.date.issued 2021-12 es_ES
dc.identifier.issn 0735-9640 es_ES
dc.identifier.uri http://hdl.handle.net/10251/186793
dc.description.abstract [EN] One of the main mechanisms regulating translation is the one based on the phosphorylation of the alpha subunit of the translation initiation factor 2 (eIF2 alpha) by the general control non-repressive 2 (GCN2) protein kinase. In yeast, this kinase binds to two scaffold proteins (GCN1 and GCN20), facilitating its activation on translating ribosomes. The homology of the three proteins exists in Arabidopsis. In this species, whereas the kinase is activated under several stress situations, the involvement of the scaffold proteins in those processes is controversial, and a new role for GCN1 in translation, independent of the phosphorylation of eIF2 alpha, has been proposed. Arabidopsis presents five genes with homology to GCN20 (ABCF1 to 5) in its genome. We show here that any of these five genes is needed for eIF2 alpha phosphorylation. Furthermore, plant phenotypes under abiotic stresses and chloroplast development suggest that ABCF3 is functionally linked with GCN1, but not with GCN2. Finally, gcn1 and abcf3 mutants share similar transcriptional reprogramming, affecting photosynthesis and stress responses. The common downregulation of regulators of the flagellin receptor FLS2 in both mutants suggest that the observed defect in pathogen-associated molecular pattern (PAMP)-induced stomatal closure of these two mutants could be mediated by these proteins. es_ES
dc.description.sponsorship This work was funded by the Spanish Ministerio de Ciencia e Innovacion (MICINN), reference BFU2011-22526. Vigya Kesari thanks the EC for an Erasmus Mundus postdoctoral fellowship. es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof Plant Molecular Biology Reporter es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Transcriptomic es_ES
dc.subject Chloroplast es_ES
dc.subject Arabidopsis translation es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title The ABCF3 Gene of Arabidopsis Is Functionally Linked with GCN1 but Not with GCN2 During Stress and Development es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s11105-021-01283-w es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2011-22526/ES/NUEVOS MECANISMOS DE TRANSMISION DE SEÑALES DURANTE EL METABOLISMO DE GLUCOSA Y LA ACIDIFICACION INTRACELULAR: AMPLIANDO LAS FUNCIONES DE LA PROTEINA FOSFATASA 1 Y LA PROTEINA/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Faus, I.; Niñoles Rodenes, R.; Kesari, V.; Gadea Vacas, J. (2021). The ABCF3 Gene of Arabidopsis Is Functionally Linked with GCN1 but Not with GCN2 During Stress and Development. Plant Molecular Biology Reporter. 39(4):663-672. https://doi.org/10.1007/s11105-021-01283-w es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s11105-021-01283-w es_ES
dc.description.upvformatpinicio 663 es_ES
dc.description.upvformatpfin 672 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 39 es_ES
dc.description.issue 4 es_ES
dc.relation.pasarela S\460369 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder MINISTERIO DE ECONOMIA Y EMPRESA es_ES
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