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PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties

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PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties

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dc.contributor.author Bauer, Leonard es_ES
dc.contributor.author Antunovic, Maja es_ES
dc.contributor.author Gallego-Ferrer, Gloria es_ES
dc.contributor.author Ivankovic, Marica es_ES
dc.contributor.author Ivankovic, Hrvoje es_ES
dc.date.accessioned 2022-10-04T18:04:57Z
dc.date.available 2022-10-04T18:04:57Z
dc.date.issued 2021-08-06 es_ES
dc.identifier.uri http://hdl.handle.net/10251/186997
dc.description.abstract [EN] Ionic substitutions within the hydroxyapatite lattice are a widely used approach to mimic the chemical composition of the bone mineral. In this work, Sr-substituted and Mg- and Sr-co-substituted calcium phosphate (CaP) scaffolds, with various levels of strontium and magnesium substitution, were prepared using the hydrothermal method at 200 degrees C. Calcium carbonate skeletons of cuttlefish bone, ammonium dihydrogenphosphate (NH4H2PO4), strontium nitrate (Sr(NO3)(2)), and magnesium perchlorate (Mg(ClO4)(2)) were used as reagents. Materials were characterized by means of X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Whole powder pattern decomposition refinements of XRD data indicated that increased magnesium content in the Mg- and Sr-co-substituted scaffolds was related to an increased proportion of the whitlockite (WH) phase in the biphasic hydroxyapatite (HAp)/WH scaffolds. In addition, refinements indicate that Sr2+ ions have replaced Ca2+ sites in the WH phase. Furthermore, PCL-coated Mg-substituted and Sr- and Mg-co-substituted scaffolds, with the HAp:WH wt. ratio of 90:10 were prepared by vacuum impregnation. Results of compression tests showed a positive impact of the WH phase and PCL coating on the mechanical properties of scaffolds. Human mesenchymal stem cells (hMSCs) were cultured on composite scaffolds in an osteogenic medium for 21 days. Immunohistochemical staining showed that Mg-Sr-CaP/PCL scaffold exhibited higher expression of collagen type I than the Mg-CaP/PCL scaffold, indicating the positive effect of Sr2+ ions on the differentiation of hMSCs, in concordance with histology results. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis confirmed an early stage of osteogenic differentiation. es_ES
dc.description.sponsorship This research was funded by the Croatian Science Foundation (project IP-2014-09-3752) and the European Structural and Investments Funds (grant KK.01.1.1.07.0014.). The authors thank Inga Urli, Faculty of Science, University of Zagreb for providing Hek293 and hMSC cells. Compression experiments were carried out at the Centre for Biomaterials and Tissue Engineering (CBIT), Universitat Politecnica de Valencia, Valencia, Spain under the PID2019-106000RB-C21/AEI/10.13039/501100011033 project. The authors would like to thank Jorge Mas-Estelles for his generous assistance. es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Materials es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Scaffold es_ES
dc.subject Hydroxyapatite es_ES
dc.subject Whitlockite es_ES
dc.subject Mg es_ES
dc.subject Sr es_ES
dc.subject Co-substitution,poly(epsilon-caprolactone) es_ES
dc.subject MSCs es_ES
dc.subject Osteogenic differentiation es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/ma14164403 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106000RB-C21/ES/HIDROGELES BIOMIMETICOS IMPRIMIBLES CON PRESENTACION DE FACTORES DE CRECIMIENTO EFICIENTE PARA ESTUDIOS DE HEPATOTOXICIDAD DE ALTO RENDIMIENTO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ESIF//KK.01.1.1.07.0014/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/HRZZ//IP-2014-09-3752/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Bauer, L.; Antunovic, M.; Gallego-Ferrer, G.; Ivankovic, M.; Ivankovic, H. (2021). PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties. Materials. 14(16):1-19. https://doi.org/10.3390/ma14164403 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/ma14164403 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 19 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 14 es_ES
dc.description.issue 16 es_ES
dc.identifier.eissn 1996-1944 es_ES
dc.identifier.pmid 34442926 es_ES
dc.identifier.pmcid PMC8398152 es_ES
dc.relation.pasarela S\459028 es_ES
dc.contributor.funder Croatian Science Foundation es_ES
dc.contributor.funder AGENCIA ESTATAL DE INVESTIGACION es_ES
dc.contributor.funder European Structural and Investment Funds es_ES


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