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dc.contributor.author | Pla, Luis | es_ES |
dc.contributor.author | Santiago-Felipe, Sara | es_ES |
dc.contributor.author | Tormo-Mas, María Ángeles | es_ES |
dc.contributor.author | Ruiz-Gaitán, Alba | es_ES |
dc.contributor.author | Peman, Javier | es_ES |
dc.contributor.author | Valentín, Eulogio | es_ES |
dc.contributor.author | Sancenón Galarza, Félix | es_ES |
dc.contributor.author | Aznar, Elena | es_ES |
dc.contributor.author | Martínez-Máñez, Ramón | es_ES |
dc.date.accessioned | 2022-10-07T18:06:45Z | |
dc.date.available | 2022-10-07T18:06:45Z | |
dc.date.issued | 2021-01-01 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/187281 | |
dc.description.abstract | [EN] Candida auris has arisen as an important multidrug-resistant fungus because of several nosocomial outbreaks and elevated rates of mortality. Accurate and rapid diagnosis of C. auris is highly desired; nevertheless, current methods often present severe limitations and produce misidentification. Herein a sensitive, selective, and time-competitive biosensor based on oligonucleotide-gated nanomaterials for effective detection of C. auris is presented. In the proposed design, a nanoporous anodic alumina scaffold is filled with the fluorescent indicator rhodamine B and the pores blocked with different oligonucleotides capable of specifically recognize C. auris genomic DNA. Gate opening modulation and cargo delivery is controlled by successful DNA recognition. C. auris is detected at a concentration as low as 6 CFU/mL allowing obtaining a diagnostic result in clinical samples in one hour with no prior DNA extraction or amplification steps. | es_ES |
dc.description.sponsorship | The authors want to thank the Spanish Government (project RTI2018-100910-B-C41 (MCIU/AEI/FEDER, UE)), the Generalitat Valenciana (project PROMETEO/2018/024), Universitat Politecnica de Valencia-Instituto de Investigacion Sanitaria La Fe (AURISGATE project) and Centro de Investigacion Biomedica en Red en Bioingenieria, Biomateriales y Nanomedicina (NANOPATH AND CANDI-EYE projects) for support. L.P thanks MINECO for his predoctoral fellowship. S.S. thanks the Instituto de Salud Carlos III and the European Social Fund for the financial support "Sara Borrell" (CD16/000237). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Nature Publishing Group | es_ES |
dc.relation.ispartof | Emerging microbes & infections | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Nanoporous anodic alumina | es_ES |
dc.subject | Oligonucleotide | es_ES |
dc.subject | Molecular gates | es_ES |
dc.subject | Candida auris | es_ES |
dc.subject | Biosensor | es_ES |
dc.subject | Rapid diagnosis | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.subject.classification | QUIMICA ORGANICA | es_ES |
dc.subject.classification | QUIMICA INORGANICA | es_ES |
dc.title | Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1080/22221751.2020.1870411 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-100910-B-C41/ES/MATERIALES POROSOS INTELIGENTES MULTIFUNCIONALES Y DISPOSITIVOS ELECTRONICOS PARA LA LIBERACION DE FARMACOS, DETECCION DE DROGAS Y BIOMARCADORES Y COMUNICACION A NANOESCALA/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//CD16%2F00237//CD16/00237/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//CD16%2F00237/ES/CD16%2F00237/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2018%2F024//Sistemas avanzados de liberación controlada/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.description.bibliographicCitation | Pla, L.; Santiago-Felipe, S.; Tormo-Mas, MÁ.; Ruiz-Gaitán, A.; Peman, J.; Valentín, E.; Sancenón Galarza, F.... (2021). Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples. Emerging microbes & infections. 10(1):407-415. https://doi.org/10.1080/22221751.2020.1870411 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1080/22221751.2020.1870411 | es_ES |
dc.description.upvformatpinicio | 407 | es_ES |
dc.description.upvformatpfin | 415 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 10 | es_ES |
dc.description.issue | 1 | es_ES |
dc.identifier.eissn | 2222-1751 | es_ES |
dc.identifier.pmid | 33372852 | es_ES |
dc.identifier.pmcid | PMC7954474 | es_ES |
dc.relation.pasarela | S\453027 | es_ES |
dc.contributor.funder | European Social Fund | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Universitat Politècnica de València | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |
dc.contributor.funder | Instituto de Investigación Sanitaria La Fe | es_ES |
dc.contributor.funder | Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina | es_ES |
upv.costeAPC | 2665 | es_ES |