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Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples

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Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples

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dc.contributor.author Pla, Luis es_ES
dc.contributor.author Santiago-Felipe, Sara es_ES
dc.contributor.author Tormo-Mas, María Ángeles es_ES
dc.contributor.author Ruiz-Gaitán, Alba es_ES
dc.contributor.author Peman, Javier es_ES
dc.contributor.author Valentín, Eulogio es_ES
dc.contributor.author Sancenón Galarza, Félix es_ES
dc.contributor.author Aznar, Elena es_ES
dc.contributor.author Martínez-Máñez, Ramón es_ES
dc.date.accessioned 2022-10-07T18:06:45Z
dc.date.available 2022-10-07T18:06:45Z
dc.date.issued 2021-01-01 es_ES
dc.identifier.uri http://hdl.handle.net/10251/187281
dc.description.abstract [EN] Candida auris has arisen as an important multidrug-resistant fungus because of several nosocomial outbreaks and elevated rates of mortality. Accurate and rapid diagnosis of C. auris is highly desired; nevertheless, current methods often present severe limitations and produce misidentification. Herein a sensitive, selective, and time-competitive biosensor based on oligonucleotide-gated nanomaterials for effective detection of C. auris is presented. In the proposed design, a nanoporous anodic alumina scaffold is filled with the fluorescent indicator rhodamine B and the pores blocked with different oligonucleotides capable of specifically recognize C. auris genomic DNA. Gate opening modulation and cargo delivery is controlled by successful DNA recognition. C. auris is detected at a concentration as low as 6 CFU/mL allowing obtaining a diagnostic result in clinical samples in one hour with no prior DNA extraction or amplification steps. es_ES
dc.description.sponsorship The authors want to thank the Spanish Government (project RTI2018-100910-B-C41 (MCIU/AEI/FEDER, UE)), the Generalitat Valenciana (project PROMETEO/2018/024), Universitat Politecnica de Valencia-Instituto de Investigacion Sanitaria La Fe (AURISGATE project) and Centro de Investigacion Biomedica en Red en Bioingenieria, Biomateriales y Nanomedicina (NANOPATH AND CANDI-EYE projects) for support. L.P thanks MINECO for his predoctoral fellowship. S.S. thanks the Instituto de Salud Carlos III and the European Social Fund for the financial support "Sara Borrell" (CD16/000237). es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Emerging microbes & infections es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Nanoporous anodic alumina es_ES
dc.subject Oligonucleotide es_ES
dc.subject Molecular gates es_ES
dc.subject Candida auris es_ES
dc.subject Biosensor es_ES
dc.subject Rapid diagnosis es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.subject.classification QUIMICA ORGANICA es_ES
dc.subject.classification QUIMICA INORGANICA es_ES
dc.title Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1080/22221751.2020.1870411 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-100910-B-C41/ES/MATERIALES POROSOS INTELIGENTES MULTIFUNCIONALES Y DISPOSITIVOS ELECTRONICOS PARA LA LIBERACION DE FARMACOS, DETECCION DE DROGAS Y BIOMARCADORES Y COMUNICACION A NANOESCALA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//CD16%2F00237//CD16/00237/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//CD16%2F00237/ES/CD16%2F00237/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2018%2F024//Sistemas avanzados de liberación controlada/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Pla, L.; Santiago-Felipe, S.; Tormo-Mas, MÁ.; Ruiz-Gaitán, A.; Peman, J.; Valentín, E.; Sancenón Galarza, F.... (2021). Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples. Emerging microbes & infections. 10(1):407-415. https://doi.org/10.1080/22221751.2020.1870411 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1080/22221751.2020.1870411 es_ES
dc.description.upvformatpinicio 407 es_ES
dc.description.upvformatpfin 415 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 10 es_ES
dc.description.issue 1 es_ES
dc.identifier.eissn 2222-1751 es_ES
dc.identifier.pmid 33372852 es_ES
dc.identifier.pmcid PMC7954474 es_ES
dc.relation.pasarela S\453027 es_ES
dc.contributor.funder European Social Fund es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Instituto de Investigación Sanitaria La Fe es_ES
dc.contributor.funder Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina es_ES
upv.costeAPC 2665 es_ES


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