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Characterisation and functional analysis of the WIF1 gene and its role in hair follicle growth and development of the Angora rabbit

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Characterisation and functional analysis of the WIF1 gene and its role in hair follicle growth and development of the Angora rabbit

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dc.contributor.author Zhao, Bohao es_ES
dc.contributor.author Li, Jiali es_ES
dc.contributor.author Zhang, Xiyu es_ES
dc.contributor.author Bao, Zhiyuan es_ES
dc.contributor.author Chen, Yang es_ES
dc.contributor.author Wu, Xinsheng es_ES
dc.date.accessioned 2022-10-10T07:12:05Z
dc.date.available 2022-10-10T07:12:05Z
dc.date.issued 2022-09-30
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/187317
dc.description.abstract [EN] Growth and development of hair follicles (HF) is a complex and dynamic process in most mammals. As HF growth and development regulate rabbit wool yield, exploring the role of genes involved in HF growth and development may be relevant. In this study, the coding sequence of the Angora rabbit (Oryctolagus cuniculus) WIF1 gene was cloned. The length of the coding region sequence was found to be 1140 bp, which encodes 379 amino acids. Bioinformatics analysis indicated that the WIF1 protein was unstable, hydrophilic and located in the extracellular region, contained a putative signal peptide and exhibited a high homology in different mammals. Moreover, WIF1 was significantly downregulated in the high wool production in the Angora rabbit group. Overexpression and knockdown studies revealed that WIF1 regulates HF growth and development-related genes and proteins, such as LEF1 and CCND1. WIF1 activated β-catenin/TCF transcriptional activity, promoted cell apoptosis and inhibited cellular proliferation. These results indicate that WIF1 might be important for HF development. This study, therefore, provides a theoretical foundation for investigating WIF1 in HF growth and development. es_ES
dc.description.sponsorship This research was funded by This research was funded by National Natural Science Foundation of China (Grant No. 32102529), China Agriculture Research System of MOF and MARA (CARS-43-A-1). es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València es_ES
dc.relation.ispartof World Rabbit Science es_ES
dc.rights Reconocimiento - No comercial - Compartir igual (by-nc-sa) es_ES
dc.subject Rabbit es_ES
dc.subject WIF1 es_ES
dc.subject Angora rabbit es_ES
dc.subject Hair follicle es_ES
dc.subject Bioinformatics es_ES
dc.subject Wnt signalling pathway es_ES
dc.title Characterisation and functional analysis of the WIF1 gene and its role in hair follicle growth and development of the Angora rabbit es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.4995/wrs.2022.17353
dc.relation.projectID info:eu-repo/grantAgreement/NSFC//32102529 es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Zhao, B.; Li, J.; Zhang, X.; Bao, Z.; Chen, Y.; Wu, X. (2022). Characterisation and functional analysis of the WIF1 gene and its role in hair follicle growth and development of the Angora rabbit. World Rabbit Science. 30(3):209-218. https://doi.org/10.4995/wrs.2022.17353 es_ES
dc.description.accrualMethod OJS es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2022.17353 es_ES
dc.description.upvformatpinicio 209 es_ES
dc.description.upvformatpfin 218 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 30 es_ES
dc.description.issue 3 es_ES
dc.identifier.eissn 1989-8886
dc.relation.pasarela OJS\17353 es_ES
dc.contributor.funder National Natural Science Foundation of China es_ES
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