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dc.contributor.author | Oliveira Leastro, Mikhail | es_ES |
dc.contributor.author | Freitas-Astua, Juliana | es_ES |
dc.contributor.author | Watanabe Kitajima, Elliot | es_ES |
dc.contributor.author | Pallás Benet, Vicente | es_ES |
dc.contributor.author | SANCHEZ NAVARRO, JESUS ANGEL | es_ES |
dc.date.accessioned | 2022-10-13T18:06:58Z | |
dc.date.available | 2022-10-13T18:06:58Z | |
dc.date.issued | 2021-02 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/187678 | |
dc.description.abstract | [EN] Although citrus leprosis disease has been known for more than a hundred years, one of its causal agents, citrus leprosis virus C2 (CiLV-C2), is poorly characterized. This study described the association of CiLV-C2 movement protein (MP) and capsid protein (p29) with biological membranes. Our findings obtained by computer predictions, chemical treatments after membrane fractionation, and biomolecular fluorescence complementation assays revealed that p29 is peripherally associated, while the MP is integrally bound to the cell membranes. Topological analyses revealed that both the p29 and MP expose their N- and C-termini to the cell cytoplasmic compartment. The implications of these results in the intracellular movement of the virus were discussed. | es_ES |
dc.description.sponsorship | This work was supported by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), proc. 2014/0845-9, 2017/50222-0, 2015/10249-1 and 2017/19898-8. This work was also supported by grant BIO2017-88321-R from the Spanish Agencia Estatal de Investigacion (AEI) and Fondo Europeo de Desarrollo Regional (FEDER), and the Prometeo Program GV2015/010 from the Generalitat Valenciana. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation.ispartof | Microorganisms | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Membrane association | es_ES |
dc.subject | Topology | es_ES |
dc.subject | Cilevirus | es_ES |
dc.subject | Movement protein | es_ES |
dc.subject | P29 capsid protein | es_ES |
dc.title | Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/microorganisms9020418 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-88321-R/ES/DESCRIFRANDO INTERACCIONES VIRUS-PLANTA ESENCIALES PARA LA SUSCEPTIBILIDAD Y%2FO RESISTENCIA EN DOS PATOSISTEMAS AGRONOMICAMENTE RELEVANTES/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2015%2F010//Interacciones RNA-proteína y proteína-proteína en procesos de desarrollo y patogénesis mediados por virus y agentes subvirales en cultivos de interés Agronómico. (ACRONIMO: RNAPROT)/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/FAPESP//2017%2F50222-0/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/FAPESP//2015%2F10249-1/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/FAPESP//2017%2F19898-8/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/FAPESP//2014%2F08459/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Oliveira Leastro, M.; Freitas-Astua, J.; Watanabe Kitajima, E.; Pallás Benet, V.; Sanchez Navarro, JA. (2021). Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins. Microorganisms. 9(2):1-9. https://doi.org/10.3390/microorganisms9020418 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/microorganisms9020418 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 9 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 9 | es_ES |
dc.description.issue | 2 | es_ES |
dc.identifier.eissn | 2076-2607 | es_ES |
dc.identifier.pmid | 33671330 | es_ES |
dc.identifier.pmcid | PMC7922530 | es_ES |
dc.relation.pasarela | S\460374 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Fundação de Amparo à Pesquisa do Estado de São Paulo | es_ES |