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Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins

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Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins

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dc.contributor.author Oliveira Leastro, Mikhail es_ES
dc.contributor.author Freitas-Astua, Juliana es_ES
dc.contributor.author Watanabe Kitajima, Elliot es_ES
dc.contributor.author Pallás Benet, Vicente es_ES
dc.contributor.author SANCHEZ NAVARRO, JESUS ANGEL es_ES
dc.date.accessioned 2022-10-13T18:06:58Z
dc.date.available 2022-10-13T18:06:58Z
dc.date.issued 2021-02 es_ES
dc.identifier.uri http://hdl.handle.net/10251/187678
dc.description.abstract [EN] Although citrus leprosis disease has been known for more than a hundred years, one of its causal agents, citrus leprosis virus C2 (CiLV-C2), is poorly characterized. This study described the association of CiLV-C2 movement protein (MP) and capsid protein (p29) with biological membranes. Our findings obtained by computer predictions, chemical treatments after membrane fractionation, and biomolecular fluorescence complementation assays revealed that p29 is peripherally associated, while the MP is integrally bound to the cell membranes. Topological analyses revealed that both the p29 and MP expose their N- and C-termini to the cell cytoplasmic compartment. The implications of these results in the intracellular movement of the virus were discussed. es_ES
dc.description.sponsorship This work was supported by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), proc. 2014/0845-9, 2017/50222-0, 2015/10249-1 and 2017/19898-8. This work was also supported by grant BIO2017-88321-R from the Spanish Agencia Estatal de Investigacion (AEI) and Fondo Europeo de Desarrollo Regional (FEDER), and the Prometeo Program GV2015/010 from the Generalitat Valenciana. es_ES
dc.language Inglés es_ES
dc.publisher MDPI es_ES
dc.relation.ispartof Microorganisms es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Membrane association es_ES
dc.subject Topology es_ES
dc.subject Cilevirus es_ES
dc.subject Movement protein es_ES
dc.subject P29 capsid protein es_ES
dc.title Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/microorganisms9020418 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-88321-R/ES/DESCRIFRANDO INTERACCIONES VIRUS-PLANTA ESENCIALES PARA LA SUSCEPTIBILIDAD Y%2FO RESISTENCIA EN DOS PATOSISTEMAS AGRONOMICAMENTE RELEVANTES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2015%2F010//Interacciones RNA-proteína y proteína-proteína en procesos de desarrollo y patogénesis mediados por virus y agentes subvirales en cultivos de interés Agronómico. (ACRONIMO: RNAPROT)/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FAPESP//2017%2F50222-0/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FAPESP//2015%2F10249-1/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FAPESP//2017%2F19898-8/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FAPESP//2014%2F08459/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Oliveira Leastro, M.; Freitas-Astua, J.; Watanabe Kitajima, E.; Pallás Benet, V.; Sanchez Navarro, JA. (2021). Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins. Microorganisms. 9(2):1-9. https://doi.org/10.3390/microorganisms9020418 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/microorganisms9020418 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 9 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 9 es_ES
dc.description.issue 2 es_ES
dc.identifier.eissn 2076-2607 es_ES
dc.identifier.pmid 33671330 es_ES
dc.identifier.pmcid PMC7922530 es_ES
dc.relation.pasarela S\460374 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Fundação de Amparo à Pesquisa do Estado de São Paulo es_ES


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