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dc.contributor.author | Pérez-Cañamás, Miryam | es_ES |
dc.contributor.author | Hevia, Elizabeth | es_ES |
dc.contributor.author | HERNANDEZ FORT, CARMEN | es_ES |
dc.contributor.author | Katsarou, Konstantina | es_ES |
dc.date.accessioned | 2022-11-07T19:01:39Z | |
dc.date.available | 2022-11-07T19:01:39Z | |
dc.date.issued | 2021 | es_ES |
dc.identifier.issn | 0022-1317 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/189381 | |
dc.description.abstract | [EN] In plants, RNA silencing functions as a potent antiviral mechanism. Virus-derived double-stranded RNAs (dsRNAs) trigger this mechanism, being cleaved by Dicer-like (DCL) enzymes into virus small RNAs (vsRNAs). These vsRNAs guide sequence-specific RNA degradation upon their incorporation into an RNA-induced silencing complex (RISC) that contains a slicer of the Argonaute (AGO) family. Host RNA dependent-RNA polymerases, particularly RDR6, strengthen antiviral silencing by generating more dsRNA templates from RISC-cleavage products that, in turn, are converted into secondary vsRNAs by DCLs. Previous work showed that Pelargonium line pattern virus (PLPV) is a very efficient inducer and target of RNA silencing as PLPV-infected Nicotiana benthamiana plants accumulate extraordinarily high amounts of vsRNAs that, strikingly, are independent of RDR6 activity. Several scenarios may explain these observations including a major contribution of dicing versus slicing for defence against PLPV, as the dicing step would not be affected by the RNA silencing suppressor encoded by the virus, a protein that acts via vsRNA sequestration. Taking advantage of the availability of lines of N. benthamiana with DCL or AGO2 functions impaired, here we have tried to get further insights into the components of the silencing machinery that are involved in anti-PLPV-silencing. Results have shown that DCL4 and, to lesser extent, DCL2 contribute to restrict viral infection. Interestingly, AGO2 apparently makes even a higher contribution in the defence against PLPV, extending the number of viruses that are affected by this particular slicer. The data support that both dicing and slicing activities participate in the host race against PLPV. | es_ES |
dc.description.sponsorship | This work was supported by grant BFU2015--70261 from the Ministerio de Economia y Competitividad (MINECO, Spain)--FEDER and PROMETEO/2019/012 from Generalitat Valenciana (GVA) (to C. H). M.P.-C. was the recipient of contracts from MINECO--FEDER and GVA, and E.H. was the recipient of a contract from MINECO--FEDER. K.K. was supported by the grant `Emblematic Action for Research in the Cretan Agrofood sector: Four Institutions, Four References' (AGRO4CRETE -2018S.01300000) held by the General Secretary for Research and Technology of Greece. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Microbiology Society | es_ES |
dc.relation.ispartof | Journal of General Virology | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | AGO2 | es_ES |
dc.subject | Antiviral RNA silencing | es_ES |
dc.subject | DCL | es_ES |
dc.subject | Nicotiana benthamiana | es_ES |
dc.subject | RNA virus | es_ES |
dc.subject | PLPV | es_ES |
dc.title | Genetic evidence for the involvement of Dicer-like 2 and 4 as well as Argonaute 2 in the Nicotiana benthamiana response against Pelargonium line pattern virus | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1099/jgv.0.001656 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BFU2015-70261-P/ES/ESTUDIO DE LA MODULACION DE LOS PROCESOS DE TRADUCCION Y DE SUPRESION DEL SILENCIAMIENTO EN UN VIRUS DE RNA DE PLANTAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GSRT//2018S.01300000//AGRO4CRETE: Emblematic Action for Research in the Cretan Agrofood sector: Four Institutions, Four References/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2019%2F012/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.description.bibliographicCitation | Pérez-Cañamás, M.; Hevia, E.; Hernandez Fort, C.; Katsarou, K. (2021). Genetic evidence for the involvement of Dicer-like 2 and 4 as well as Argonaute 2 in the Nicotiana benthamiana response against Pelargonium line pattern virus. Journal of General Virology. 102(10):1-9. https://doi.org/10.1099/jgv.0.001656 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1099/jgv.0.001656 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 9 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 102 | es_ES |
dc.description.issue | 10 | es_ES |
dc.identifier.pmid | 34623234 | es_ES |
dc.identifier.pmcid | PMC8604191 | es_ES |
dc.relation.pasarela | S\459944 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |
dc.contributor.funder | General Secretariat for Research and Technology, Grecia | es_ES |