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An all-in-one point-of-care testing device for multiplexed detection of respiratory infections

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An all-in-one point-of-care testing device for multiplexed detection of respiratory infections

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dc.contributor.author Teixeira, William es_ES
dc.contributor.author Pallás-Tamarit, Yeray es_ES
dc.contributor.author Juste-Dolz, Augusto Miguel es_ES
dc.contributor.author Sena-Torralba, Amadeo es_ES
dc.contributor.author Gozalbo-Rovira, Roberto es_ES
dc.contributor.author Rodríguez-Díaz, Jesús es_ES
dc.contributor.author Navarro, David es_ES
dc.contributor.author Carrascosa Rubio, Javier es_ES
dc.contributor.author Giménez-Romero, David es_ES
dc.contributor.author Maquieira Catala, Angel es_ES
dc.contributor.author Morais, Sergi es_ES
dc.date.accessioned 2023-02-23T19:00:46Z
dc.date.available 2023-02-23T19:00:46Z
dc.date.issued 2022-10-01 es_ES
dc.identifier.issn 0956-5663 es_ES
dc.identifier.uri http://hdl.handle.net/10251/192049
dc.description.abstract [EN] The impact of the COVID-19 pandemic has reinforced the need for rapid, cost-effective, and reliable point-of-care testing (POCT) devices for massive population screening. The co-circulation of SARS-CoV-2 with several seasonal respiratory viruses highlights the need for multiplexed biosensing approaches. Herein, we present a fast and robust all-in-one POCT device for parallel viral antigen and serological analysis. The biosensing approach consists of a functionalized polycarbonate disc-shaped surface with microfluidic structures, where specific bioreagents are immobilized in microarray format, and a portable optoelectronic analyzer. The biosensor quantifies the concentration of viral antigens and specific immunoglobulins G and M for SARS-CoV-2, influenza A/B, adenovirus, and respiratory syncytial virus, using 30¿¿L of a sample. The semi-automated analysis of 6 samples is performed in 30¿min. Validation studies performed with 135 serum samples and 147 nasopharyngeal specimens reveal high diagnostic sensitivity (98¿100%) and specificity (84¿98%), achieving an excellent agreement (¿¿=¿0.937) with commercial immunoassays, which complies with the World Health Organization criteria for POC COVID-19 diagnostic tests. The versatility of the POCT device paves the way for the detection of other pathogens and analytes in the incoming post-pandemic world, integrating specific bioreagents against different variants of concerns and interests. es_ES
dc.description.sponsorship W. T and A. J-D acknowledge the financial support for the Ph.D. studies (PAID-19-01-06 and FPI-UPV 2017 grants, respectively). S.M awards grant PID2019-110713RB-I00 funded by MCIN/AEI/10.13039/501100011033 and by "ERDF A way of making Europe." A.M awards grant DOGV no. 8815, May 19, 2020, pp. 16712-16726. The research was also supported by the Generalitat Valenciana (PROMETEO/2020/094, Proyecto "88/2021"). The authors gratefully acknowledge the outstanding contribution of Alicia Parra, Violeta Espada, Roberto Renuncio, and Jose A. Marzal for collecting the nasopharyngeal swab samples from the Health Centre of the Universitat Politecnica de Valencia. We also gratefully thank Dr. L. Mira and G. Rios for carefully reading this manuscript and the four anonymous reviewers for their insightful comments and suggestions. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Biosensors and Bioelectronics es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject COVID-19 es_ES
dc.subject SARS-CoV-2 es_ES
dc.subject Influenza es_ES
dc.subject Adenovirus es_ES
dc.subject Respiratory syncytial virus es_ES
dc.subject Microfluidics es_ES
dc.subject Immunoassay es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title An all-in-one point-of-care testing device for multiplexed detection of respiratory infections es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.bios.2022.114454 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110713RB-I00/ES/HACIA EL BIOSENSADO HOLOGRAFICO AVANZADO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GENERALITAT VALENCIANA//COVID-19%2F5//ADAPTACION Y VALIDACION DE SISTEMAS BIOSENSORES CON TRANSDUCCIÓN OPTICA-CD Y VISCOELASTICA-QCM PARA LA DETECCION DE SARS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//FPI 2017/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//PAID-19-01-06/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2020%2F094//HOLOGRAFÍA. UNA VIA PARA AFRONTAR NUEVOS RETOS EN BIOSENSADO/ es_ES
dc.rights.accessRights Embargado es_ES
dc.date.embargoEndDate 2024-06-08 es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Teixeira, W.; Pallás-Tamarit, Y.; Juste-Dolz, AM.; Sena-Torralba, A.; Gozalbo-Rovira, R.; Rodríguez-Díaz, J.; Navarro, D.... (2022). An all-in-one point-of-care testing device for multiplexed detection of respiratory infections. Biosensors and Bioelectronics. 213:1-7. https://doi.org/10.1016/j.bios.2022.114454 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.bios.2022.114454 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 7 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 213 es_ES
dc.identifier.pmid 35696866 es_ES
dc.identifier.pmcid PMC9176175 es_ES
dc.relation.pasarela S\466773 es_ES
dc.contributor.funder GENERALITAT VALENCIANA es_ES
dc.contributor.funder AGENCIA ESTATAL DE INVESTIGACION es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
dc.subject.ods 03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades es_ES


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