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A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes

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A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes

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dc.contributor.author Cuenca-Ortolá, Irene es_ES
dc.contributor.author Martinez-Rojas, Beatriz es_ES
dc.contributor.author Moreno Manzano, Victoria es_ES
dc.contributor.author García Castelló, Marcos es_ES
dc.contributor.author Monleón Pradas, Manuel es_ES
dc.contributor.author Martínez-Ramos, Cristina es_ES
dc.contributor.author Más Estellés, Jorge es_ES
dc.date.accessioned 2023-04-20T18:00:40Z
dc.date.available 2023-04-20T18:00:40Z
dc.date.issued 2022-11 es_ES
dc.identifier.uri http://hdl.handle.net/10251/192891
dc.description.abstract [EN] Neural progenitor cells (NPCs) have been shown to serve as an efficient therapeutic strategy in different cell therapy approaches, including spinal cord injury treatment. Despite the reported beneficial effects of NPC transplantation, the low survival and differentiation rates constrain important limitations. Herein, a new methodology has been developed to overcome both limitations by applying a combination of wireless electrical and magnetic stimulation to NPCs seeded on aligned poly(lactic acid) nanofibrous scaffolds for in vitro cell conditioning prior transplantation. Two stimulation patterns were tested and compared, continuous (long stimulus applied once a day) and intermittent (short stimulus applied three times a day). The results show that applied continuous stimulation promotes NPC proliferation and preferential differentiation into oligodendrocytic and neuronal lineages. A neural-like phenotypic induction was observed when compared to unstimulated NPCs. In contrast, intermittent stimulation patterns did not affect NPC proliferation and differentiation to oligodendrocytes or astrocytes morphology with a detrimental effect on neuronal differentiation. This study provides a new approach of using a combination of electric and magnetic stimulation to induce proliferation and further neuronal differentiation, which would improve therapy outcomes in disorders such as spinal cord injury. es_ES
dc.description.sponsorship Grants RTI2018-095872-B-C21/ERDF and RTI2018-095872-B-C22/ERDF funded by MCIN/AEI//10.13039/501100011033 and by ERDF A way of making Europe. This project was also funded by Project 964562 H2020 FetOpen program. Irene Cuenca Ortola acknowledges the grant PRE2019-090716 funded by MCIN/AEI/10.13039/501100011033 and by ESF Investing in your future. B. Martinez Rojas was supported by a grant from the Conselleria de Educacion, Investigacion, Cultura y Deporte de la Generalitat Valenciana and the European Social Fundation ACIF/2019/120. es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Biomedicines es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Polylactic acid es_ES
dc.subject Aligned substrates es_ES
dc.subject Neural progenitor cells es_ES
dc.subject Magnetic stimulation es_ES
dc.subject Electric stimulation es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/biomedicines10112736 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095872-B-C21/ES/NUEVO BIOMATERIAL BIO-ACTIVO PARA LA REGENERACION DE LESIONES MEDULARES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACIF%2F2019%2F120/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095872-B-C22/ES/NUEVO DISPOSITIVO BIOACTIVO PARA LA REGENERACION DE LESIONES DE LA MEDULA ESPINAL/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI//PRE2019-090716/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/964562/EU es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escola Tècnica Superior d'Enginyeria Informàtica es_ES
dc.description.bibliographicCitation Cuenca-Ortolá, I.; Martinez-Rojas, B.; Moreno Manzano, V.; García Castelló, M.; Monleón Pradas, M.; Martínez-Ramos, C.; Más Estellés, J. (2022). A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes. Biomedicines. 10(11):1-19. https://doi.org/10.3390/biomedicines10112736 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/biomedicines10112736 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 19 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 10 es_ES
dc.description.issue 11 es_ES
dc.identifier.eissn 2227-9059 es_ES
dc.identifier.pmid 36359255 es_ES
dc.identifier.pmcid PMC9687775 es_ES
dc.relation.pasarela S\485212 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder European Social Fund es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder AGENCIA ESTATAL DE INVESTIGACION es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
upv.costeAPC 2722,5 es_ES


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