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dc.contributor.author | Salazar-Sarasua, Blanca | es_ES |
dc.contributor.author | López-Martín, María Jesús | es_ES |
dc.contributor.author | Roque Mesa, Edelin Marta | es_ES |
dc.contributor.author | Hamza, Rim | es_ES |
dc.contributor.author | Cañas Clemente, Luís Antonio | es_ES |
dc.contributor.author | BELTRAN PORTER, JOSE PIO | es_ES |
dc.contributor.author | Gómez Mena, María Concepción | es_ES |
dc.date.accessioned | 2023-04-27T18:01:22Z | |
dc.date.available | 2023-04-27T18:01:22Z | |
dc.date.issued | 2022-12 | es_ES |
dc.identifier.issn | 0960-7412 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/193001 | |
dc.description.abstract | [EN] The tapetum is a specialized layer of cells within the anther, adjacent to the sporogenous tissue. During its short life, it provides nutrients, molecules and materials to the pollen mother cells and microsporocytes, being essential during callose degradation and pollen wall formation. The interaction between the tapetum and sporogenous cells in Solanum lycopersicum (tomato) plants, despite its importance for breeding purposes, is poorly understood. To investigate this process, gene editing was used to generate loss-of-function mutants that showed the complete and specific absence of tapetal cells. These plants were obtained targeting the previously uncharacterized Solyc03g097530 (SlTPD1) gene, essential for tapetum specification in tomato plants. In the absence of tapetum, sporogenous cells developed and callose deposition was observed. However, sporocytes failed to undergo the process of meiosis and finally degenerated, leading to male sterility. Transcriptomic analysis conducted in mutant anthers lacking tapetum revealed the downregulation of a set of genes related to redox homeostasis. Indeed, mutant anthers showed a reduction in the accumulation of reactive oxygen species (ROS) at early stages and altered activity of ROS-scavenging enzymes. The results obtained highlight the importance of the tapetal tissue in maintaining redox homeostasis during male gametogenesis in tomato plants. | es_ES |
dc.description.sponsorship | This work was supported by grant RTI2018-094280-B-I00 funded by Ministerio de Ciencia e Innovacion (MCIN)/Agencia Estatal de Investigacion (AEI)/10.13039/501100011033 and by Fondo Europeo de Desarrollo Regional (FEDER) `A way of making Europe'. We thank Aureliano Bombarely for his help in the conversion of gene IDs into Solyc identifiers, Diego Orzaez for providing GoldenBraid parts and Maricruz Rochina for expert technical assistance during the project. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Blackwell Publishing | es_ES |
dc.relation.ispartof | The Plant Journal | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Solanum lycopersicum | es_ES |
dc.subject | Tapetum | es_ES |
dc.subject | Pollen | es_ES |
dc.subject | ROS | es_ES |
dc.subject | TPD1 | es_ES |
dc.subject | Anther | es_ES |
dc.subject | Male sterility | es_ES |
dc.title | The tapetal tissue is essential for the maintenance of redox homeostasis during microgametogenesis in tomato | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1111/tpj.16014 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/Ministerio de Ciencia, Innovación y Universidades//RTI2018-094280-B-100//Edición de genes de tomate implicados en la gametogénesis masculina: disección del potencial biotecnológico de la androesterilidad en la obtención de frutos sin semillas./ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Salazar-Sarasua, B.; López-Martín, MJ.; Roque Mesa, EM.; Hamza, R.; Cañas Clemente, LA.; Beltran Porter, JP.; Gómez Mena, MC. (2022). The tapetal tissue is essential for the maintenance of redox homeostasis during microgametogenesis in tomato. The Plant Journal. 112(5):1281-1297. https://doi.org/10.1111/tpj.16014 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1111/tpj.16014 | es_ES |
dc.description.upvformatpinicio | 1281 | es_ES |
dc.description.upvformatpfin | 1297 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 112 | es_ES |
dc.description.issue | 5 | es_ES |
dc.identifier.pmid | 36307971 | es_ES |
dc.identifier.pmcid | PMC10100220 | es_ES |
dc.relation.pasarela | S\479541 | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades | es_ES |