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dc.contributor.author | Marquez-Molins, Joan | es_ES |
dc.contributor.author | Hernandez-Azurdia, Andrea Gabriela | es_ES |
dc.contributor.author | Urrutia-Perez, María | es_ES |
dc.contributor.author | Pallás Benet, Vicente | es_ES |
dc.contributor.author | Gomez, Gustavo Germán | es_ES |
dc.date.accessioned | 2023-04-27T18:01:27Z | |
dc.date.available | 2023-04-27T18:01:27Z | |
dc.date.issued | 2022-10 | es_ES |
dc.identifier.issn | 0960-7412 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/193004 | |
dc.description.abstract | [EN] Gene silencing for functional studies in plants has been largely facilitated by manipulating viral genomes with inserts from host genes to trigger virus-induced gene silencing (VIGS) against the corresponding mRNAs. However, viral genomes encode multiple proteins and can disrupt plant homeostasis by interfering with endogenous cell mechanisms. To try to circumvent this functional limitation, we have developed a silencing method based on the minimal autonomously-infectious nucleic acids currently known: viroids, which lack proven coding capability. The genome of Eggplant latent viroid, an asymptomatic viroid, was manipulated with insertions ranging between 21 and 42 nucleotides. Our results show that, although larger insertions might be tolerated, the maintenance of the secondary structure appears to be critical for viroid genome stability. Remarkably, these modified ELVd molecules are able to induce systemic infection promoting the silencing of target genes in eggplant. Inspired by the design of artificial microRNAs, we have developed a simple and standardized procedure to generate stable insertions into the ELVd genome capable of silencing a specific target gene. Analogously to VIGS, we have termed our approach viroid-induced gene silencing, and demonstrate that it is a promising tool for dissecting gene functions in eggplant. | es_ES |
dc.description.sponsorship | This work was supported by the Spanish Agencia Estatal de Investigacion (co-supported by FEDER) Grants PID2019-104126RB-I00 (GG) and PID2020-115571RB-I00 (VP). JM-M and AGH-A were recipients of a pre-doctoral contract from the Generalitat Valenciana (ACIF-2017-114 and ACIF-2021-202). The funders had no role in the experiment design, data analysis, decision to publish, or preparation of the manuscript. The authors want to acknowledge the valuable comments related to new control experiments made by two anonymous reviewers. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Blackwell Publishing | es_ES |
dc.relation.ispartof | The Plant Journal | es_ES |
dc.rights | Reconocimiento - No comercial (by-nc) | es_ES |
dc.subject | Viroids as biotechnological tools | es_ES |
dc.subject | Gene silencing | es_ES |
dc.subject | RNA replicon | es_ES |
dc.subject | Avsunviroidae | es_ES |
dc.subject | Regulation of plant gene expression | es_ES |
dc.subject | Technical advance | es_ES |
dc.title | A circular RNA vector for targeted plant gene silencing based on an asymptomatic viroid | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1111/tpj.15929 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104126RB-I00/ES/REDES REGULADORAS GENICAS INFLUENCIADAS POR EL AMBIENTE IMPLICADAS EN EL CONTROL DE LA REPUESTA A ESTRES EN MELON/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//ACIF%2F2021%2F202/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115571RB-I00/ES/LA MODIFICACION N6-METILADENOSINA (M6A) DEL RNA Y EL DIRECCIONAMIENTO DUAL A CLOROPLASTOS Y MITOCONDRIAS COMO MECANISMOS REGULADORES EN LA BIOLOGIA DE LOS VIRUS RNA DE PLANTAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//ACIF%2F2017%2F114/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.description.bibliographicCitation | Marquez-Molins, J.; Hernandez-Azurdia, AG.; Urrutia-Perez, M.; Pallás Benet, V.; Gomez, GG. (2022). A circular RNA vector for targeted plant gene silencing based on an asymptomatic viroid. The Plant Journal. 112(1):284-293. https://doi.org/10.1111/tpj.15929 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1111/tpj.15929 | es_ES |
dc.description.upvformatpinicio | 284 | es_ES |
dc.description.upvformatpfin | 293 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 112 | es_ES |
dc.description.issue | 1 | es_ES |
dc.identifier.pmid | 35916236 | es_ES |
dc.identifier.pmcid | PMC9804161 | es_ES |
dc.relation.pasarela | S\487428 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |