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Characterization of Arabidopsis Post-Glycosylphosphatidylinositol Attachment to Proteins Phospholipase 3 Like Genes

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Characterization of Arabidopsis Post-Glycosylphosphatidylinositol Attachment to Proteins Phospholipase 3 Like Genes

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dc.contributor.author Bernat-Silvestre, Cesar es_ES
dc.contributor.author Ma, Yingxuan es_ES
dc.contributor.author Johnson, Kim es_ES
dc.contributor.author Ferrando Monleón, Alejandro Ramón es_ES
dc.contributor.author Aniento, Fernando es_ES
dc.contributor.author Marcote, María Jesús es_ES
dc.date.accessioned 2023-05-03T18:02:12Z
dc.date.available 2023-05-03T18:02:12Z
dc.date.issued 2022-02-11 es_ES
dc.identifier.uri http://hdl.handle.net/10251/193103
dc.description.abstract [EN] Lipid remodeling of Glycosylphosphatidylinositol (GPI) anchors is required for their maturation and may influence the localization and function of GPI-anchored proteins (GPI-APs). Maturation of GPI-anchors is well characterized in animals and fungi but very little is known about this process in plants. In yeast, the GPI-lipid remodeling occurs entirely at the ER and is initiated by the remodeling enzyme Bst1p (Post-Glycosylphosphatidylinositol Attachment to Proteins inositol deacylase 1 -PGAP1- in mammals and Arabidopsis). Next, the remodeling enzyme Per1p (Post-Glycosylphosphatidylinositol Attachment to Proteins phospholipase 3 -PGAP3- in mammals) removes a short, unsaturated fatty acid of phosphatidylinositol (PI) that is replaced with a very long-chain saturated fatty acid or ceramide to complete lipid remodeling. In mammals, lipid remodeling starts at the ER and is completed at the Golgi apparatus. Studies of the Arabidopsis PGAP1 gene showed that the lipid remodeling of the GPI anchor is critical for the final localization of GPI-APs. Here we characterized loss-of-function mutants of Arabidopsis Per1/PGAP3 like genes (AtPGAP3A and AtPGAP3B). Our results suggest that PGAP3A function is required for the efficient transport of GPI-anchored proteins from the ER to the plasma membrane/cell wall. In addition, loss of function of PGAP3A increases susceptibility to salt and osmotic stresses that may be due to the altered localization of GPI-APs in this mutant. Furthermore, PGAP3B complements a yeast strain lacking PER1 gene suggesting that PGAP3B and Per1p are functional orthologs. Finally, subcellular localization studies suggest that PGAP3A and PGAP3B cycle between the ER and the Golgi apparatus. es_ES
dc.description.sponsorship FA and MM were supported by the Ministerio de Economía y Competitividad (grant no. BFU2016-76607P), Ministerio de Ciencia e Innovación, MICINN/ AEI/10.13039/501100011033 (grant no. PID2020-113847GB-100), and Generalitat Valenciana (AICO/2020/187). CB-S was recipient of a fellowship from Ministerio de Ciencia, Innovación y Universidades (FPU program). CB-S was also recipient of an EMBO short-term fellowship and a short-term fellowship from Ministerio de Ciencia, Innovación y Universidades. es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation.ispartof Frontiers in Plant Science es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Glycosylphosphatidylinositol (GPI),GPI-anchored proteins,Per1p,PGAP3,lipid remodeling,Arabidopsis es_ES
dc.title Characterization of Arabidopsis Post-Glycosylphosphatidylinositol Attachment to Proteins Phospholipase 3 Like Genes es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fpls.2022.817915 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//AICO%2F2020%2F187/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2016-76607P/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//PID2020-113847GB-100/ es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Bernat-Silvestre, C.; Ma, Y.; Johnson, K.; Ferrando Monleón, AR.; Aniento, F.; Marcote, MJ. (2022). Characterization of Arabidopsis Post-Glycosylphosphatidylinositol Attachment to Proteins Phospholipase 3 Like Genes. Frontiers in Plant Science. 13:1-15. https://doi.org/10.3389/fpls.2022.817915 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fpls.2022.817915 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 15 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 13 es_ES
dc.identifier.eissn 1664-462X es_ES
dc.identifier.pmid 35222477 es_ES
dc.identifier.pmcid 35222477 es_ES
dc.relation.pasarela S\485954 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Ministerio de Universidades es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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