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Multiplexed analytical approaches to beta-lactam allergy in vitro testing standardization

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Multiplexed analytical approaches to beta-lactam allergy in vitro testing standardization

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dc.contributor.author Juárez-Rodríguez, María José es_ES
dc.contributor.author Ibáñez-Echevarría, Ethel es_ES
dc.contributor.author Hernández-Fernández de Rojas, Dolores es_ES
dc.contributor.author Maquieira, Angel es_ES
dc.contributor.author Morais, Sergi es_ES
dc.date.accessioned 2023-05-04T18:01:57Z
dc.date.available 2023-05-04T18:01:57Z
dc.date.issued 2021-08-15 es_ES
dc.identifier.issn 0003-2670 es_ES
dc.identifier.uri http://hdl.handle.net/10251/193133
dc.description.abstract [EN] The suspicion of beta-lactam allergy directly contributes to the prescription of antibiotics that diverge from the guidelines, increasing antimicrobial resistance, one of the biggest threats to global health. In vitro quantification of specific IgE is very useful for monitoring allergy, as it confirms or rules out immediate beta-lactam drug allergy and helps find safe alternative antibiotic stewardship. However, reliable in vitro quantification of specific IgE to beta-lactam antibiotics by immunoassay is challenging because of the difficulty of having selective immunoreagents, mainly beta-lactam antigens, and its low concentration levels in serum. Thus, reliable and sensitive in vitro tests for multiplex detection of allergy to different beta-lactam antibiotics is currently essential for clinical diagnosis. Nevertheless, the lack of standardization of quantitative in vitro methods makes the comparison and interpretation of the results difficult. Here, as proof of concept, we report an improved multiplex microimmunoassay for beta-lactam allergy in vitro testing standardization. The results revealed that homologous calibration allows reliable quantification of specific IgE in human serum at very low concentrations (144 ng L-1). Moreover, the reproducibility of the results increases 2-fold using an internal standard, achieving accurate quantitative information: 93% and 106% recovery for penicillin and amoxicillin, respectively. We simultaneously evaluated the reliability of the improved multiplexed in vitro method in a cohort of 40 human serum samples and achieved excellent agreement (0.99) with a currently used in vitro test. es_ES
dc.description.sponsorship M.J.J acknowledges financial support from the FPIprogram from Universitat Politecnica de Valencia. This research was supported by the H2020 program (project COBIOPHAD, grant agreement No. 688448) , being an initiative of the Photonics Public Private Partnership; Agencia Estatal de Investigacian (PID2019-110713RB-I0 0, FEDER) ; program UPVLa FE 2019 (P105 VALBIOAL). es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Analytica Chimica Acta es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject IgE es_ES
dc.subject Standardization es_ES
dc.subject Drug allergy es_ES
dc.subject Diagnostic es_ES
dc.subject Microarray es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Multiplexed analytical approaches to beta-lactam allergy in vitro testing standardization es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.aca.2021.338656 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110713RB-I00/ES/HACIA EL BIOSENSADO HOLOGRAFICO AVANZADO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UNIVERSIDAD POLITECNICA DE VALENCIA//UPV-FE-PI05//VALIDACION DE UN SISTEMA BIOFOTONICO PARA EL DIAGNOSTICO IN VITRO DE ALERGIA A ANTIBIOTICOS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/688448/EU es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Hospital Universitari i Politècnic La Fe//PI05 VALBIOAL/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Juárez-Rodríguez, MJ.; Ibáñez-Echevarría, E.; Hernández-Fernández De Rojas, D.; Maquieira, A.; Morais, S. (2021). Multiplexed analytical approaches to beta-lactam allergy in vitro testing standardization. Analytica Chimica Acta. 1173:1-8. https://doi.org/10.1016/j.aca.2021.338656 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.aca.2021.338656 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 8 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 1173 es_ES
dc.identifier.pmid 34172156 es_ES
dc.relation.pasarela S\438330 es_ES
dc.contributor.funder AGENCIA ESTATAL DE INVESTIGACION es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder COMISION DE LAS COMUNIDADES EUROPEA es_ES
dc.contributor.funder UNIVERSIDAD POLITECNICA DE VALENCIA es_ES
dc.contributor.funder Hospital Universitari i Politècnic La Fe es_ES


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