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A serralysin-like protein of Candidatus Liberibacter asiaticus modulates components of the bacterial extracellular matrix

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A serralysin-like protein of Candidatus Liberibacter asiaticus modulates components of the bacterial extracellular matrix

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dc.contributor.author Garcia, Lucila es_ES
dc.contributor.author Molina, Maria Celeste es_ES
dc.contributor.author Padgett-Pagliai, Kaylie Allyson es_ES
dc.contributor.author Torres, Pablo S. es_ES
dc.contributor.author Bruna, Roberto es_ES
dc.contributor.author García Véscovi, Eleonora es_ES
dc.contributor.author González, Claudio F. es_ES
dc.contributor.author Gadea Vacas, José es_ES
dc.contributor.author Marano, María Rosa es_ES
dc.date.accessioned 2023-05-04T18:01:59Z
dc.date.available 2023-05-04T18:01:59Z
dc.date.issued 2022-10-19 es_ES
dc.identifier.issn 1664-302X es_ES
dc.identifier.uri http://hdl.handle.net/10251/193135
dc.description.abstract [EN] Huanglongbing (HLB), the current major threat for Citrus species, is caused by intracellular alphaproteobacteria of the genus Candidatus Liberibacter (CaL), with CaL asiaticus (CLas) being the most prevalent species. This bacterium inhabits phloem cells and is transmitted by the psyllid Diaphorina citri. A gene encoding a putative serralysin-like metalloprotease (CLIBASIA_01345) was identified in the CLas genome. The expression levels of this gene were found to be higher in citrus leaves than in psyllids, suggesting a function for this protease in adaptation to the plant environment. Here, we study the putative role of CLas-serralysin (Las1345) as virulence factor. We first assayed whether Las1345 could be secreted by two different surrogate bacteria, Rhizobium leguminosarum bv. viciae A34 (A34) and Serratia marcescens. The protein was detected only in the cellular fraction of A34 and S. marcescens expressing Las1345, and increased protease activity of those bacteria by 2.55 and 4.25-fold, respectively. In contrast, Las1345 expressed in Nicotiana benthamiana leaves did not show protease activity nor alterations in the cell membrane, suggesting that Las1345 do not function as a protease in the plant cell. Las1345 expression negatively regulated cell motility, exopolysaccharide production, and biofilm formation in Xanthomonas campestris pv. campestris (Xcc). This bacterial phenotype was correlated with reduced growth and survival on leaf surfaces as well as reduced disease symptoms in N. benthamiana and Arabidopsis. These results support a model where Las1345 could modify extracellular components to adapt bacterial shape and appendages to the phloem environment, thus contributing to virulence. es_ES
dc.description.sponsorship This work was mainly supported by the Agencia Nacional de Promoción Científica y Tecnológica (PICT-2018-03051) to MRM and (PICT-2016-3108) to LG, by COST (European Cooperation in Science and Technology, COST Action) to JG and by the USDA National Institute of Food and Agriculture (Plant Biotic Interactions Program; award number 2017-03060) to CG. es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation.ispartof Frontiers in Microbiology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Biofilm es_ES
dc.subject Huanglongbing es_ES
dc.subject Protease es_ES
dc.subject Surrogate bacteria es_ES
dc.subject Virulence factor es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title A serralysin-like protein of Candidatus Liberibacter asiaticus modulates components of the bacterial extracellular matrix es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fmicb.2022.1006962 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/USDA//2017-03060/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ANPCyT//PICT-2018-03051/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ANPCyT//PICT-2016-3108/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Garcia, L.; Molina, MC.; Padgett-Pagliai, KA.; Torres, PS.; Bruna, R.; García Véscovi, E.; González, CF.... (2022). A serralysin-like protein of Candidatus Liberibacter asiaticus modulates components of the bacterial extracellular matrix. Frontiers in Microbiology. 13:1-19. https://doi.org/10.3389/fmicb.2022.1006962 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fmicb.2022.1006962 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 19 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 13 es_ES
dc.identifier.pmid 36338045 es_ES
dc.identifier.pmcid PMC9627510 es_ES
dc.relation.pasarela S\479128 es_ES
dc.contributor.funder U.S. Department of Agriculture es_ES
dc.contributor.funder European Cooperation in Science and Technology es_ES
dc.contributor.funder Agencia Nacional de Promoción Científica y Tecnológica, Argentina es_ES


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