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Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells

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Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells

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dc.contributor.author Guillot-Ferriols, María Teresa es_ES
dc.contributor.author García-Briega, María Inmaculada es_ES
dc.contributor.author Tolosa, Laia es_ES
dc.contributor.author Costa, Carlos M. es_ES
dc.contributor.author Lanceros-Méndez, Senentxu es_ES
dc.contributor.author Gómez Ribelles, José Luís es_ES
dc.contributor.author Gallego-Ferrer, Gloria es_ES
dc.date.accessioned 2023-05-08T18:01:57Z
dc.date.available 2023-05-08T18:01:57Z
dc.date.issued 2022-10 es_ES
dc.identifier.uri http://hdl.handle.net/10251/193207
dc.description.abstract [EN] Mesenchymal stem cells (MSCs) osteogenic commitment before injection enhances bone regeneration therapy results. Piezoelectric stimulation may be an effective cue to promote MSCs pre-differentiation, and poly(vinylidene) fluoride (PVDF) cell culture supports, when combined with CoFe2O4 (CFO), offer a wireless in vitro stimulation strategy. Under an external magnetic field, CFO shift and magnetostriction deform the polymer matrix varying the polymer surface charge due to the piezoelectric effect. To test the effect of piezoelectric stimulation on MSCs, our approach is based on a gelatin hydrogel with embedded MSCs and PVDF-CFO electroactive microspheres. Microspheres were produced by electrospray technique, favouring CFO incorporation, crystallisation in beta-phase (85%) and a crystallinity degree of around 55%. The absence of cytotoxicity of the 3D construct was confirmed 24 h after cell encapsulation. Cells were viable, evenly distributed in the hydrogel matrix and surrounded by microspheres, allowing local stimulation. Hydrogels were stimulated using a magnetic bioreactor, and no significant changes were observed in MSCs proliferation in the short or long term. Nevertheless, piezoelectric stimulation upregulated RUNX2 expression after 7 days, indicating the activation of the osteogenic differentiation pathway. These results open the door for optimising a stimulation protocol allowing the application of the magnetically activated 3D electroactive cell culture support for MSCs pre-differentiation before transplantation. es_ES
dc.description.sponsorship This research was funded by Spanish State Research Agency (AEI), G.G.F. acknowledges the support of the PID2019-106000RB-C21/AEI/10.13039/501100011033 project, J.L.G.R. and S.L.-M. acknowledge the support of the PID2019-106099RB-C41 and -C43/AEI/10.13039/501100011033 projects. The CIBER-BBN initiative is funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development. M.G.-F. received government funding for her doctoral thesis, grant number BES-2017-080398. The authors thank FCT (Fundacao para a Ciencia e a Tecnologia) for financial support under the framework of Strategic Funding grant number UID/FIS/04650/2021, projects PTDC/FIS-MAC/28157/2017 and POCI-01-0145-FEDER-007688, and contract under the Stimulus of Scientific Employment, Individual Support 2020.04028 CEECIND (C.M.C.). es_ES
dc.language Inglés es_ES
dc.publisher MDPI es_ES
dc.relation.ispartof Gels es_ES
dc.relation.uri https://riunet.upv.es/handle/10251/188261
dc.rights Reconocimiento (by) es_ES
dc.subject Mesenchymal stem cells es_ES
dc.subject Osteoblastogenesis es_ES
dc.subject Piezoelectricity es_ES
dc.subject Poly(vinylidene) fluoride es_ES
dc.subject Hydrogel es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/gels8100680 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106000RB-C21/ES/HIDROGELES BIOMIMETICOS IMPRIMIBLES CON PRESENTACION DE FACTORES DE CRECIMIENTO EFICIENTE PARA ESTUDIOS DE HEPATOTOXICIDAD DE ALTO RENDIMIENTO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI//BES-2017-080398//AYUDA PARA CONTRATOS PREDOCTORALES PARA LA FORMACION DE DOCTORES 2017-GUILLOT FERRIOLS. PROYECTO: BIOMATERIALES PIEZOELECTRICOS PARA LA DIFERENCIACION CELULAR EN INTERFACES CELULA-MATERIAL ELECTRICAMENTE ACTIVAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106099RB-C41/ES/MICROGELES BIOMIMETICOS PARA EL ESTUDIO DE LA GENERACION DE RESISTENCIAS A FARMACOS EN EL MIELOMA MULTIPLE/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//2020.04028 CEECIND/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106099RB-C43/ES/DESARROLLO DE ANDAMIAJES BIOMIMETICOS ACTIVOS PARA EL ESTUDIO DE MICROENTORNO DE TUMOR EN OSTEOSARCOMA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//UID%2FFIS%2F04650%2F2021/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FFIS-MAC%2F28157%2F2017/PT es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//POCI-01-0145-FEDER007688/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials es_ES
dc.description.bibliographicCitation Guillot-Ferriols, MT.; García-Briega, MI.; Tolosa, L.; Costa, CM.; Lanceros-Méndez, S.; Gómez Ribelles, JL.; Gallego-Ferrer, G. (2022). Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells. Gels. 8(10):1-17. https://doi.org/10.3390/gels8100680 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/gels8100680 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 17 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.description.issue 10 es_ES
dc.identifier.eissn 2310-2861 es_ES
dc.identifier.pmid 36286181 es_ES
dc.identifier.pmcid PMC9602007 es_ES
dc.relation.pasarela S\480372 es_ES
dc.contributor.funder AGENCIA ESTATAL DE INVESTIGACION es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Fundação para a Ciência e a Tecnologia, Portugal es_ES


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