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Production of Potyvirus-Derived Nanoparticles Decorated with a Nanobody in Biofactory Plants

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Production of Potyvirus-Derived Nanoparticles Decorated with a Nanobody in Biofactory Plants

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dc.contributor.author Martí, Mari Carmen es_ES
dc.contributor.author Merwaiss, Fernando es_ES
dc.contributor.author Butkovic, Anamarija es_ES
dc.contributor.author Daròs, José-Antonio es_ES
dc.date.accessioned 2023-05-08T18:02:19Z
dc.date.available 2023-05-08T18:02:19Z
dc.date.issued 2022-03-31 es_ES
dc.identifier.uri http://hdl.handle.net/10251/193226
dc.description.abstract [EN] Viral nanoparticles (VNPs) have recently attracted attention for their use as building blocks for novel materials to support a range of functions of potential interest in nanotechnology and medicine. Viral capsids are ideal for presenting small epitopes by inserting them at an appropriate site on the selected coat protein (CP). VNPs presenting antibodies on their surfaces are considered highly promising tools for therapeutic and diagnostic purposes. Due to their size, nanobodies are an interesting alternative to classic antibodies for surface presentation. Nanobodies are the variable domains of heavy-chain (VHH) antibodies from animals belonging to the family Camelidae, which have several properties that make them attractive therapeutic molecules, such as their small size, simple structure, and high affinity and specificity. In this work, we have produced genetically encoded VNPs derived from two different potyviruses¿the largest group of RNA viruses that infect plants¿decorated with nanobodies. We have created a VNP derived from zucchini yellow mosaic virus (ZYMV) decorated with a nanobody against the green fluorescent protein (GFP) in zucchini (Cucurbita pepo) plants. As reported for other viruses, the expression of ZYMV-derived VNPs decorated with this nanobody was only made possible by including a picornavirus 2A splicing peptide between the fused proteins, which resulted in a mixed population of unmodified and decorated CPs. We have also produced tobacco etch virus (TEV)-derived VNPs in Nicotiana benthamiana plants decorated with the same nanobody against GFP. Strikingly, in this case, VNPs could be assembled by direct fusion of the nanobody to the viral CP with no 2A splicing involved, likely resulting in fully decorated VNPs. For both expression systems, correct assembly and purification of the recombinant VNPs was confirmed by transmission electron microscope; the functionality of the CP-fused nanobody was assessed by western blot and binding assays. In sum, here we report the production of genetically encoded plant-derived VNPs decorated with a nanobody. This system may be an attractive alternative for the sustainable production in plants of nanobody-containing nanomaterials for diagnostic and therapeutic purposes. es_ES
dc.description.sponsorship This research was supported by grant PID 2020-114691RB-I00 from the Ministerio de Ciencia e Innovacion (Spain; co-financed by the European Region Development Fund) through the Agencia Estatal de Investigacion. MM is the recipient of a predoctoral contract (FPU16/05294) from the Ministerio de Educacion, Cultura y Deporte (Spain). es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media S.A. es_ES
dc.relation.ispartof Frontiers in Bioengineering and Biotechnology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Viral nanoparticle es_ES
dc.subject VNP es_ES
dc.subject Plant virus es_ES
dc.subject Nanobody es_ES
dc.subject Potyvirus es_ES
dc.subject Zucchini yellow mosaic virus es_ES
dc.subject Tobacco etch virus es_ES
dc.title Production of Potyvirus-Derived Nanoparticles Decorated with a Nanobody in Biofactory Plants es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fbioe.2022.877363 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MECD//FPU16%2F05294/ES/FPU16%2F05294/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//PID2020-114691RB-I00 //Biotecnología de virus de plantas: vectores virales y estrategias de resistencia/ es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Martí, MC.; Merwaiss, F.; Butkovic, A.; Daròs, J. (2022). Production of Potyvirus-Derived Nanoparticles Decorated with a Nanobody in Biofactory Plants. Frontiers in Bioengineering and Biotechnology. 10:1-13. https://doi.org/10.3389/fbioe.2022.877363 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fbioe.2022.877363 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 13 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 10 es_ES
dc.identifier.eissn 2296-4185 es_ES
dc.identifier.pmid 35433643 es_ES
dc.identifier.pmcid PMC9008781 es_ES
dc.relation.pasarela S\459229 es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Ministerio de Educación, Cultura y Deporte es_ES


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