Llopis-Lorente, A.; García-Fernández, A.; Murillo-Cremaes, N.; Hortelao, A.; Patiño, T.; Villalonga, R.; Sancenón Galarza, F.... (2019). Enzyme-Powered Gated Mesoporous Silica Nanomotors for On-Command Intracellular Payload Delivery. ACS Nano. 13(10):12171-12183. https://doi.org/10.1021/acsnano.9b06706
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/193436
Title:
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Enzyme-Powered Gated Mesoporous Silica Nanomotors for On-Command Intracellular Payload Delivery
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Author:
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Llopis-Lorente, Antoni
García-Fernández, Alba
Murillo-Cremaes, N.
Hortelao, A.C.
Patiño, T.
Villalonga, R.
Sancenón Galarza, Félix
Martínez-Máñez, Ramón
Sanchez, S.
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UPV Unit:
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Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials
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Issued date:
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Abstract:
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[EN] The introduction of stimuli-responsive cargo release capabilities on self-propelled micro- and nano- motors holds enormous potential in a number of applications in the biomedical field. Herein, we report the preparation ...[+]
[EN] The introduction of stimuli-responsive cargo release capabilities on self-propelled micro- and nano- motors holds enormous potential in a number of applications in the biomedical field. Herein, we report the preparation of mesoporous silica nano-particles gated with pH-responsive supramolecular nanovalves and equipped with urease enzymes which act as chemical engines to power the nanomotors. The nanoparticles are loaded with different cargo molecules ([Ru(bpy)(3)]Cl-2 (bpy = 2,2'-bipyridine) or doxorubicin), grafted with benzimidazole groups on the outer surface, and capped by the formation of inclusion complexes between benzimidazole and cyclodextrin-modified urease. The nanomotor exhibits enhanced Brownian motion in the presence of urea. Moreover, no cargo is released at neutral pH, even in the presence of the biofuel urea, due to the blockage of the pores by the bulky benzimidazole:cyclodextrin-urease caps. Cargo delivery is only triggered on-command at acidic pH due to the protonation of benzimidazole groups, the dethreading of the supramolecular nanovalves, and the subsequent uncapping of the nanoparticles. Studies with HeLa cells indicate that the presence of biofuel urea enhances nanoparticle internalization and both [Ru(bpy)(3)]Cl-2 or doxorubicin intracellular release due to the acidity of lysosomal compartments. Gated enzyme-powered nanomotors shown here display some of the requirements for ideal drug delivery carriers such as the capacity to self-propel and the ability to "sense" the environment and deliver the payload on demand in response to predefined stimuli.
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Subjects:
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Nanomotors
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Drug delivery,controlled release
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Nanocarriers
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Enzymatic catalysis
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Stimuli-responsive nanomaterials
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Gatekeepers
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Copyrigths:
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Reserva de todos los derechos
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Source:
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ACS Nano. (issn:
1936-0851
)
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DOI:
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10.1021/acsnano.9b06706
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Publisher:
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American Chemical Society
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Publisher version:
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https://doi.org/10.1021/acsnano.9b06706
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Project ID:
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info:eu-repo/grantAgreement/MINECO//CTQ2014-58989-P/ES/BIONANORROBOTS QUIMICAMENTE PROGRAMADOS Y CONTROLADOS POR ENZIMAS/
...[+]
info:eu-repo/grantAgreement/MINECO//CTQ2014-58989-P/ES/BIONANORROBOTS QUIMICAMENTE PROGRAMADOS Y CONTROLADOS POR ENZIMAS/
info:eu-repo/grantAgreement/GENERALITAT VALENCIANA//PROMETEO%2F2018%2F024//SISTEMAS AVANZADOS DE LIBERACION CONTROLADA/
info:eu-repo/grantAgreement/MINECO//CTQ2015-68879-R/ES/SISTEMAS LAB-ON-A-CHIP BASADOS EN MICRO-NANOMOTORES PARA EL DIAGNOSTICO DE ENFERMEDADES/
info:eu-repo/grantAgreement/EC//MSCA-IF2018//DNA-bots/
info:eu-repo/grantAgreement/MINECO//CTQ2015-71936-REDT/ES/MODIFICACION QUIMICA DEL GRAFENO PARA NUEVAS PROPIEDADES Y APLICACIONES/
info:eu-repo/grantAgreement/GVA//PROMETEO%2F2018%2F024//Sistemas avanzados de liberación controlada/
info:eu-repo/grantAgreement/MINECO//CTQ2015-72471-EXP/ES/NANOMOTORES DE NANOPARTICULAS MESOPOROSAS IMPULSADOS POR ENZIMAS/
info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F061/
info:eu-repo/grantAgreement/MINECO//MAT2015-64139-C4-1-R/ES/NANOMATERIALES INTELIGENTES, SONDAS Y DISPOSITIVOS PARA EL DESARROLLO INTEGRADO DE NUEVAS HERRAMIENTAS APLICADAS AL CAMPO BIOMEDICO/
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Thanks:
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A.L.-L. is grateful to La Caixa Banking Foundation for his
Ph.D. grant. A.G.-F. thanks the Spanish government for her FPU
fellowship. The authors are grateful to the Spanish Government
(MINECO Projects MAT2015-64139-C4-1, ...[+]
A.L.-L. is grateful to La Caixa Banking Foundation for his
Ph.D. grant. A.G.-F. thanks the Spanish government for her FPU
fellowship. The authors are grateful to the Spanish Government
(MINECO Projects MAT2015-64139-C4-1, CTQ2014-58989-
PCTQ2015-71936-REDT, CTQ2015-68879-R (MICRODIA)
and CTQ2015-72471-EXP (Enzwim)), the BBVA foundation
(MEDIROBOTS), the CERCA Programme by the Generalitat
de Catalunya, and the Generalitat Valenciana (Project
PROMETEO/2018/024 and PROMETEOII/2014/061) for
support. T.P. thanks MINECO for the Juan de la Cierva
postdoctoral fellowship and the European Union's Horizon
2020 research and innovation program, under the Marie
Sk¿odowska-Curie Individual Fellowship (H2020-MSCA-IF2018, DNA-bots). A.C.H. thanks MINECO for the Severo
Ochoa fellowship. The authors would like to thank A. Miguel Lopez for the development of the python code for motion
analysis.
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Type:
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Artículo
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