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dc.contributor.author | Gisbert Roca, Fernando | es_ES |
dc.contributor.author | Serrano Requena, Sara | es_ES |
dc.contributor.author | Monleón Pradas, Manuel | es_ES |
dc.contributor.author | Martínez-Ramos, Cristina | es_ES |
dc.date.accessioned | 2023-05-25T18:01:13Z | |
dc.date.available | 2023-05-25T18:01:13Z | |
dc.date.issued | 2022-06 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/193601 | |
dc.description.abstract | [EN] Nerve regeneration is a slow process that needs to be guided for distances greater than 5 mm. For this reason, different strategies are being studied to guide axonal growth and accelerate the axonal growth rate. In this study, we employ an electroconductive fibrillar substrate that is able to topographically guide axonal growth while accelerating the axonal growth rate when subjected to an exogenous electric field. Dorsal root ganglia were seeded in co-culture with Schwann cells on a substrate of polylactic acid microfibers coated with the electroconductive polymer polypyrrole, adding gold microfibers to increase its electrical conductivity. The substrate is capable of guiding axonal growth in a highly aligned manner and, when subjected to an electrical stimulation, an improvement in axonal growth is observed. As a result, an increase in the maximum length of the axons of 19.2% and an increase in the area occupied by the axons of 40% were obtained. In addition, an upregulation of the genes related to axon guidance, axogenesis, Schwann cells, proliferation and neurotrophins was observed for the electrically stimulated group. Therefore, our device is a good candidate for nerve regeneration therapies. | es_ES |
dc.description.sponsorship | This research was funded by the Spanish Government's State Research Agency (AEI) through project RTI2018-095872-B-C22/ERDF and by RISEUP project FetOpen in H2020 Program: RISEUP 964562 H2020 FetOpen program. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI AG | es_ES |
dc.relation.ispartof | International Journal of Molecular Sciences | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Electrical stimulation | es_ES |
dc.subject | Alternating current | es_ES |
dc.subject | Axonal growth | es_ES |
dc.subject | Aligned substrates | es_ES |
dc.subject | Polylactic acid | es_ES |
dc.subject | Polypyrrole | es_ES |
dc.subject | Schwann cells | es_ES |
dc.subject.classification | MAQUINAS Y MOTORES TERMICOS | es_ES |
dc.title | Electrical Stimulation Increases Axonal Growth from Dorsal Root Ganglia Co-Cultured with Schwann Cells in Highly Aligned PLA-PPy-Au Microfiber Substrates | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/ijms23126362 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095872-B-C22/ES/NUEVO DISPOSITIVO BIOACTIVO PARA LA REGENERACION DE LESIONES DE LA MEDULA ESPINAL/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/964562/EU | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials | es_ES |
dc.description.bibliographicCitation | Gisbert Roca, F.; Serrano Requena, S.; Monleón Pradas, M.; Martínez-Ramos, C. (2022). Electrical Stimulation Increases Axonal Growth from Dorsal Root Ganglia Co-Cultured with Schwann Cells in Highly Aligned PLA-PPy-Au Microfiber Substrates. International Journal of Molecular Sciences. 23:1-22. https://doi.org/10.3390/ijms23126362 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/ijms23126362 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 22 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 23 | es_ES |
dc.identifier.eissn | 1422-0067 | es_ES |
dc.identifier.pmid | 35742806 | es_ES |
dc.identifier.pmcid | PMC9223746 | es_ES |
dc.relation.pasarela | S\466643 | es_ES |
dc.contributor.funder | AGENCIA ESTATAL DE INVESTIGACION | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | COMISION DE LAS COMUNIDADES EUROPEA | es_ES |