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Rapid Oxford Nanopore Technologies MinION Sequencing Workflow for Campylobacter jejuni Identification in Broilers on Site-A Proof-of-Concept Study

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Rapid Oxford Nanopore Technologies MinION Sequencing Workflow for Campylobacter jejuni Identification in Broilers on Site-A Proof-of-Concept Study

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dc.contributor.author Marin, Clara es_ES
dc.contributor.author Marco-Jiménez, Francisco es_ES
dc.contributor.author Martínez-Priego, Llucia es_ES
dc.contributor.author De Marco-Romero, Griselda es_ES
dc.contributor.author Soriano-Chirona, Vicente es_ES
dc.contributor.author Lorenzo-Rebenaque, Laura es_ES
dc.contributor.author D Auria, Giuseppe es_ES
dc.date.accessioned 2023-05-25T18:01:18Z
dc.date.available 2023-05-25T18:01:18Z
dc.date.issued 2022-08 es_ES
dc.identifier.uri http://hdl.handle.net/10251/193604
dc.description.abstract [EN] Campylobacter is recognised as one of the most important foodborne bacteria, with a worldwide health and socioeconomic impact. This bacterium is one of the most important zoonotic players in poultry, where efficient and fast detection methods are required. Current official culture methods for Campylobacter enumeration in poultry usually include >44 h of culture and >72 h for identification, thus requiring at least five working shifts (ISO/TS 10272-2:2017). Here, we have assembled a portable sequencing kit composed of the Bento Lab and the MinION and developed a workflow for on-site farm use that is able to detect and report the presence of Campylobacter from caecal samples in less than five hours from sampling time, as well as the relationship of Campylobacter with other caecal microbes. Beyond that, our workflow may offer a cost-effective and practical method of microbiologically monitoring poultry at the farm. These results would demonstrate the possibility of carrying out rapid on-site screening to monitor the health status of the poultry farm/flock during the production chain. es_ES
dc.description.sponsorship Laura Lorenzo-Rebenaque was supported by a research grant from the Generalitat Valenciana-Fondo Social Europeo (ACIF/2020/376). es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Animals es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Poultry es_ES
dc.subject Foodborne es_ES
dc.subject 16S RNA es_ES
dc.subject Microbiota es_ES
dc.subject Bento Lab es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Rapid Oxford Nanopore Technologies MinION Sequencing Workflow for Campylobacter jejuni Identification in Broilers on Site-A Proof-of-Concept Study es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/ani12162065 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACIF%2F2020%2F376/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Marin, C.; Marco-Jiménez, F.; Martínez-Priego, L.; De Marco-Romero, G.; Soriano-Chirona, V.; Lorenzo-Rebenaque, L.; D Auria, G. (2022). Rapid Oxford Nanopore Technologies MinION Sequencing Workflow for Campylobacter jejuni Identification in Broilers on Site-A Proof-of-Concept Study. Animals. 12(16):1-13. https://doi.org/10.3390/ani12162065 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/ani12162065 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 13 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 12 es_ES
dc.description.issue 16 es_ES
dc.identifier.eissn 2076-2615 es_ES
dc.identifier.pmid 36009653 es_ES
dc.identifier.pmcid PMC9405271 es_ES
dc.relation.pasarela S\477388 es_ES
dc.contributor.funder Generalitat Valenciana es_ES


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