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Simultaneous detection of less frequent waterborne parasitic protozoa in reused wastewater using amplicon sequencing and qPCR techniques

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Simultaneous detection of less frequent waterborne parasitic protozoa in reused wastewater using amplicon sequencing and qPCR techniques

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dc.contributor.author Moreno-Mesonero, L. es_ES
dc.contributor.author Amoros, Inmaculada es_ES
dc.contributor.author Moreno Trigos, Mª Yolanda es_ES
dc.contributor.author Alonso Molina, José Luís es_ES
dc.date.accessioned 2023-05-26T18:01:34Z
dc.date.available 2023-05-26T18:01:34Z
dc.date.issued 2022-07-15 es_ES
dc.identifier.issn 0301-4797 es_ES
dc.identifier.uri http://hdl.handle.net/10251/193622
dc.description.abstract [EN] Waterborne parasitic protozoa (WPP) infections have a worldwide distribution and are a source for epidemic and endemic human diseases. Although a variety of protozoa are commonly detected in wastewater and cited as causative agents of outbreaks, effluents from wastewater treatment plants (WWTPs) used for irrigation can contain other pathogenic protozoa that are not currently being controlled. The lack of control on a routine basis using rapid and sensitive methods to detect these parasites in water may keep them under-recognized. This study focused on using molecular tools, 18 S rRNA amplicon-based sequencing and qPCR, to characterize WPP distribution in wastewater samples from urban WWTPs used for irrigation. A total of eight wastewater samples (from secondary and tertiary disinfection treatment effluents) were collected. Potentially pathogenic protozoa identified by 18 S rRNA sequencing and/or qPCR in the analyzed samples included Acanthamoeba spp., Blastocystis sp., Entamoeba coli, Entamoeba dispar, Entamoeba hartmanni, Giardia intestinalis assemblage A and Toxoplasma gondii Positive results by qPCR were in non-quantifiable levels. Blastocystis sp. was the most represented protozoa among the sequences retrieved from the amplicon sequencing. Blastocystis ST1 and ST2 were the most abundant subtypes among the obtained OTUs. Moreover, Blastocystis sp. ST3, ST4, ST6 and ST8 were also detected, although in lower abundances. Results of this study showed that WWTP effluents used for irrigation can provide a source of WPP. es_ES
dc.description.sponsorship This work has been financed through the project funded by the Spanish Ministry of Economy and Competitiveness (MINECO), in the frame of the collaborative international consortium JPIW2013-095-C0302 -METAWATER of the Water Challenges for a Changing World Joint Programming Initiative (Water JPI) Pilot Call. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Journal of Environmental Management es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Irrigation water es_ES
dc.subject Wastewater es_ES
dc.subject Waterborne parasitic protozoa es_ES
dc.subject Blastocystis subtypesq es_ES
dc.subject PCRAmplicon sequencing es_ES
dc.title Simultaneous detection of less frequent waterborne parasitic protozoa in reused wastewater using amplicon sequencing and qPCR techniques es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.jenvman.2022.115029 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//JPIW2013-095-C03-02/ES/NUEVOS INSTRUMENTOS MOLECULARES Y DE METAGENOMICA PARA LA IDENTIFICACION Y CONTROL A ESCALA EUROPEA DE CONTAMINANTES MICROBIANOS EMERGENTES EN AGUA DE RIEGO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//JPIW2013-095-C03-02//NUEVOS INSTRUMENTOS MOLECULARES Y DE METAGENOMICA PARA LA IDENTIFICACION Y CONTROL A ESCALA EUROPEA DE CONTAMINANTES MICROBIANOS EMERGENTES EN AGUA DE RIEGO/ es_ES
dc.rights.accessRights Embargado es_ES
dc.date.embargoEndDate 2024-07-15 es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient es_ES
dc.description.bibliographicCitation Moreno-Mesonero, L.; Amoros, I.; Moreno Trigos, MY.; Alonso Molina, JL. (2022). Simultaneous detection of less frequent waterborne parasitic protozoa in reused wastewater using amplicon sequencing and qPCR techniques. Journal of Environmental Management. 314:1-9. https://doi.org/10.1016/j.jenvman.2022.115029 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.jenvman.2022.115029 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 9 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 314 es_ES
dc.identifier.pmid 35430514 es_ES
dc.relation.pasarela S\468672 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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