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dc.contributor.author | Jiménez-Falcao, Sandra![]() |
es_ES |
dc.contributor.author | Villalonga, Anabel![]() |
es_ES |
dc.contributor.author | Arévalo-Villena, María![]() |
es_ES |
dc.contributor.author | Briones-Pérez, Ana![]() |
es_ES |
dc.contributor.author | Martínez-Máñez, Ramón![]() |
es_ES |
dc.contributor.author | Martínez-Ruíz, Paloma![]() |
es_ES |
dc.contributor.author | Villalonga, Reynaldo![]() |
es_ES |
dc.date.accessioned | 2023-05-29T18:02:59Z | |
dc.date.available | 2023-05-29T18:02:59Z | |
dc.date.issued | 2020-01-15 | es_ES |
dc.identifier.issn | 0925-4005 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/193722 | |
dc.description.abstract | [EN] We report herein the construction of an integrated and sensitive enzyme-powered colorimetric nanosensor for the quantification of living Saccharomyces cerevisiae. The nanodevice is provided with a pH-sensitive supramolecular nanovalve in capped benzimidazole-functionalized mesoporous silica nanoparticles with beta-cyclodextrincoated gold nanoparticles containing adatamantane-modified glucose oxidase as bioactive element. The sensing approach relies on the ability of living S. cerevisiae cells to produce invertase, which hydrolyzes sucrose to fructose and glucose. Enzymatic transformation of glucose to gluconic acid on the nanosensor surface results in the opening of the pores and delivery of an encapsulated dye, as a consequence of the local decrease in the pH. The nanosensor allows the detection of the living yeast in the range of 8.10(2) - 4.10(8) CFU/mL, exhibiting high selectivity and reproducibility, and retaining full sensing capacity after 2 weeks of storage. The nanodevice has also been successfully validated in wine samples spiked with S. cerevisiae. | es_ES |
dc.description.sponsorship | Financial support from the Spanish Ministry of Economy and Competitiveness (MINECO Projects CTQ2017-87954-P and RTI2018-100910-B-C41), the Junta de Comunidades de Castilla La Mancha (JCCM Project POII-2014-011-A), the Comunidad de Madrid (Project IND2017/BMD-7642) and the Generalitat Valenciana (Project PROMETEO2018/024) is gratefully acknowledged. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Sensors and Actuators B Chemical | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Saccharomyces cerevisiae | es_ES |
dc.subject | Nanosensor | es_ES |
dc.subject | Mesoporous silica nanoparticles | es_ES |
dc.subject | Enzyme | es_ES |
dc.subject | Wine | es_ES |
dc.subject.classification | QUIMICA INORGANICA | es_ES |
dc.title | Enzyme-controlled mesoporous nanosensor for the detection of living Saccharomyces cerevisiae | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.snb.2019.127197 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2017-87954-P/ES/NANOMAQUINAS INTELIGENTES BASADAS EN NANOMATERIALES JANUS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GENERALITAT VALENCIANA//PROMETEO%2F2018%2F024//SISTEMAS AVANZADOS DE LIBERACION CONTROLADA/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-100910-B-C41/ES/MATERIALES POROSOS INTELIGENTES MULTIFUNCIONALES Y DISPOSITIVOS ELECTRONICOS PARA LA LIBERACION DE FARMACOS, DETECCION DE DROGAS Y BIOMARCADORES Y COMUNICACION A NANOESCALA/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/CAM//IND2017%2FBMD-7642/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/JCCM//JCCM Project POII-2014-011-A/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials | es_ES |
dc.description.bibliographicCitation | Jiménez-Falcao, S.; Villalonga, A.; Arévalo-Villena, M.; Briones-Pérez, A.; Martínez-Máñez, R.; Martínez-Ruíz, P.; Villalonga, R. (2020). Enzyme-controlled mesoporous nanosensor for the detection of living Saccharomyces cerevisiae. Sensors and Actuators B Chemical. 303:1-7. https://doi.org/10.1016/j.snb.2019.127197 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.snb.2019.127197 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 7 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 303 | es_ES |
dc.relation.pasarela | S\418248 | es_ES |
dc.contributor.funder | Comunidad de Madrid | es_ES |
dc.contributor.funder | GENERALITAT VALENCIANA | es_ES |
dc.contributor.funder | AGENCIA ESTATAL DE INVESTIGACION | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | Junta de Comunidades de Castilla-La Mancha | es_ES |