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Examining the effects of Salmonella phage on the caecal microbiota and metabolome features in Salmonella-free broilers

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Examining the effects of Salmonella phage on the caecal microbiota and metabolome features in Salmonella-free broilers

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dc.contributor.author Lorenzo-Rebenaque, Laura es_ES
dc.contributor.author Casto-Rebollo, Cristina es_ES
dc.contributor.author Diretto, Gianfranco es_ES
dc.contributor.author Frusciante, Sarah es_ES
dc.contributor.author Rodríguez, Juan Carlos es_ES
dc.contributor.author Ventero, María-Paz es_ES
dc.contributor.author Vega, Santiago es_ES
dc.contributor.author Molina-Pardines, Carmen es_ES
dc.contributor.author Marin, Clara es_ES
dc.contributor.author Marco-Jiménez, Francisco es_ES
dc.date.accessioned 2023-07-04T18:01:44Z
dc.date.available 2023-07-04T18:01:44Z
dc.date.issued 2022-11-10 es_ES
dc.identifier.uri http://hdl.handle.net/10251/194677
dc.description.abstract [EN] Bacteriophages selectively infect and kill their target bacterial host, being a promising approach to controlling zoonotic bacteria in poultry production. To ensure confidence in its use, fundamental questions of safety and toxicity monitoring of phage therapy should be raised. Due to its high specificity, a minimal impact on the gut ecology is expected; however, more in-depth research into key parameters that influence the success of phage interventions has been needed to reach a consensus on the impact of bacteriophage therapy in the gut. In this context, this study aimed to investigate the interaction of phages with animals; more specifically, we compared the caecum microbiome and metabolome after a Salmonella phage challenge in Salmonella-free broilers, evaluating the role of the phage administration route. To this end, we employed 45 caecum content samples from a previous study where Salmonella phages were administered via drinking water or feed for 24 h from 4, 5 to 6-weeks-old broilers. High-throughput 16S rRNA gene sequencing showed a high level of similarity (beta diversity) but revealed a significant change in alpha diversity between broilers with Salmonella-phage administered in the drinking water and control. Our results showed that the phages affected only a few genera of the microbiota¿s structure, regardless of the administration route. Among these, we found a significant increase in Streptococcus and Sellimonas in the drinking water and Lactobacillus, Anaeroplasma and Clostridia_vadinBB60_group in the feed. Nevertheless, the LC-HRMS-based metabolomics analyses revealed that despite few genera were significantly affected, a substantial number of metabolites, especially in the phage administered in the drinking water were significantly altered (64 and 14 in the drinking water and feed groups, respectively). Overall, our study shows that preventive therapy with bacteriophages minimally alters the caecal microbiota but significantly impacts their metabolites, regardless of the route of administration. es_ES
dc.description.sponsorship LL-R was supported by a research grant from the Generalitat Valenciana-Fondo Social Europeo (ACIF/2020/376) and by a research mobility grant (IV convocatoria de ayudas para la movilidad internacional de investigadores en formacion de la CEU escuela internacional de doctorado (CEINDO)-Banco Santander). We would like to thank the Centre for Poultry Quality and Animal Feed of the Valencian Community (CECAV), and University CEU-Cardenal Herrera (INDI 21/35) for their financial support, and to Juan Carlos Rodriguez and his research group for supporting the microbiome experimental procedures (MSD Grant ISP 60386). es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media es_ES
dc.relation.ispartof Frontiers in Genetics es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Bacteriophages es_ES
dc.subject Poultry es_ES
dc.subject Omic sciences es_ES
dc.subject High throughput sequencing es_ES
dc.subject Microbiome es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Examining the effects of Salmonella phage on the caecal microbiota and metabolome features in Salmonella-free broilers es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fgene.2022.1060713 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACIF%2F2020%2F376/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Universidad CEU Cardenal Herrera//INDI 21%2F35/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Lorenzo-Rebenaque, L.; Casto-Rebollo, C.; Diretto, G.; Frusciante, S.; Rodríguez, JC.; Ventero, M.; Vega, S.... (2022). Examining the effects of Salmonella phage on the caecal microbiota and metabolome features in Salmonella-free broilers. Frontiers in Genetics. 13:1-13. https://doi.org/10.3389/fgene.2022.1060713 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fgene.2022.1060713 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 13 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 13 es_ES
dc.identifier.eissn 1664-8021 es_ES
dc.identifier.pmid 36437955 es_ES
dc.identifier.pmcid PMC9691336 es_ES
dc.relation.pasarela S\477393 es_ES
dc.contributor.funder Banco Santander es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Universidad CEU Cardenal Herrera es_ES


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