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Orchid fleck dichorhavirus movement protein shows RNA silencing suppressor activity.

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Orchid fleck dichorhavirus movement protein shows RNA silencing suppressor activity.

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dc.contributor.author Oliveira Leastro, Mikhail es_ES
dc.contributor.author Pallás Benet, Vicente es_ES
dc.contributor.author Sánchez-Navarro, Jesús Ángel es_ES
dc.date.accessioned 2023-09-21T18:05:05Z
dc.date.available 2023-09-21T18:05:05Z
dc.date.issued 2022-11-18 es_ES
dc.identifier.issn 0022-1317 es_ES
dc.identifier.uri http://hdl.handle.net/10251/196904
dc.description.abstract [EN] To counteract RNA interference-mediated antiviral defence, virus genomes evolved to express proteins that inhibit this plant defence mechanism. Using six independent biological approaches, we show that orchid fleck dichorhavirus citrus strain (OFV-citrus) movement protein (MP) may act as a viral suppressor of RNA silencing (VSR). By using the alfalfa mosaic virus (AMV) RNA 3 expression vector, it was observed that the MP triggered necrosis response in transgenic tobacco leaves and increased the viral RNA (vRNA) accumulation. The use of the potato virus X (PVX) expression system revealed that the cis expression of MP increased both the severity of the PVX infection and the accumulation of PVX RNAs, further supporting that MP could act as an RNA silencing suppressor (RSS). From the analysis of the RSS-defective turnip crinkle virus (TCV), we do not find local RSS activity for MP, suggesting a link between MP suppressor activity and the prevention of systemic silencing. In the analysis of local suppressive activity using the GFP-based agroinfiltration assay in Nicotiana benthamiana (16 c line), we do not identify local RSS activity for the five OFV RNA1-encoded proteins. However, when evaluating the small interfering RNA (siRNA) accumulation, we find that the expression of MP significantly reduces the accumulation of GFP-derived siRNA. Finally, we examine whether the MP can prevent systemic silencing in 16c plants. Our findings show that MP inhibits the long-distance spread of RNA silencing, but does not affect the short-distance spread. Together, our findings indicate that MP is part of OFV's counter-defence mechanism, acting mainly in the prevention of systemic long-distance silencing. This work presents the first report of a VSR for a member of the genus Dichorhavirus. es_ES
dc.description.sponsorship This work was supported by grant PID2020-115571RB-100 from the Spanish MCIN/AEI/10.13039/501100011033 granting agency and Fondo Europeo de Desarrollo Regional (FEDER), and by the company INVESTIR IMOVEIS LDTA from Brasilia, Brazil. es_ES
dc.language Inglés es_ES
dc.publisher Microbiology Society es_ES
dc.relation.ispartof Journal of General Virology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Dichorhavirus es_ES
dc.subject OFV es_ES
dc.subject Movement protein es_ES
dc.subject RNA silencing es_ES
dc.subject Viral suppressors of RNA silencing es_ES
dc.title Orchid fleck dichorhavirus movement protein shows RNA silencing suppressor activity. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1099/jgv.0.001805 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115571RB-I00/ES/LA MODIFICACION N6-METILADENOSINA (M6A) DEL RNA Y EL DIRECCIONAMIENTO DUAL A CLOROPLASTOS Y MITOCONDRIAS COMO MECANISMOS REGULADORES EN LA BIOLOGIA DE LOS VIRUS RNA DE PLANTAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Oliveira Leastro, M.; Pallás Benet, V.; Sánchez-Navarro, JÁ. (2022). Orchid fleck dichorhavirus movement protein shows RNA silencing suppressor activity. Journal of General Virology. 103(11):1-14. https://doi.org/10.1099/jgv.0.001805 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1099/jgv.0.001805 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 14 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 103 es_ES
dc.description.issue 11 es_ES
dc.identifier.pmid 36399122 es_ES
dc.relation.pasarela S\487435 es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES


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