- -

Quantitative and qualitative study of endogenous and exogenous growth regulators in eggplant (Solanum melongena) microspore cultures

RiuNet: Repositorio Institucional de la Universidad Politécnica de Valencia

Compartir/Enviar a

Citas

Estadísticas

  • Estadisticas de Uso

Quantitative and qualitative study of endogenous and exogenous growth regulators in eggplant (Solanum melongena) microspore cultures

Mostrar el registro sencillo del ítem

Ficheros en el ítem

Thumbnail
Nombre: Calabuig-SernaCam ...
Tamaño: 1.525Mb
Formato: PDF
Descripción: Versión editorial
Abrir
dc.contributor.author Calabuig-Serna, Antonio es_ES
dc.contributor.author Camacho-Fernández, Carolina es_ES
dc.contributor.author Mir Moreno, Ricardo es_ES
dc.contributor.author Porcel, R es_ES
dc.contributor.author Carrera, Esther es_ES
dc.contributor.author LOPEZ DIAZ, ISABEL es_ES
dc.contributor.author Seguí-Simarro, Jose M. es_ES
dc.date.accessioned 2023-09-29T18:04:50Z
dc.date.available 2023-09-29T18:04:50Z
dc.date.issued 2022-03 es_ES
dc.identifier.issn 0167-6903 es_ES
dc.identifier.uri http://hdl.handle.net/10251/197360
dc.description.abstract [EN] In eggplant microspore embryogenesis, embryos are produced and then transformed into undifferentiated calli, instead of developing as true embryos. This is the main current bottleneck that precludes this process from being efficient. In this work we aimed to shed light on the factors involved in the successful in vitro development of eggplant haploid embryos by evaluating the role of growth regulators (GRs) in this process. We analyzed the endogenous levels of different GRs, including auxins, cytokinins and gibberelins, as well as salicylic, jasmonic and abscisic acid, in microspores and microspore-derived embryos at different culture stages. We also analyzed the same GR profiles in leaf and anther wall tissues of different eggplant backgrounds. Finally, we assessed the application of different GR combinations to the culture medium. Our results showed that in eggplant there are no genotype-specific endogenous GR profiles that can be associated to a high embryogenic response. Instead, the embryogenic response seems related to different GR accumulation patterns during in vitro culture. The changes observed in the endogenous levels of salicylic and abscisic acid were not related to the embryo transition. There were, however, changes in the levels of indole acetic acid and dihydrozeatin. The best GR combination to promote callus production was 0.5 mg/L 1-naphthaleneacetic acid (NAA) and 0.5 mg/L 6-benzylaminopurine (BAP). A 20% reduction of NAA and BAP reduced embryo production but produced structures more anatomically similar to embryos. These results shed light on the role of GRs during the development of microspore-derived embryos in eggplant microspore cultures. es_ES
dc.description.sponsorship Funding for open access charge: CRUE-Universitat Politecnica de Valencia. This work was supported by Grant PID2020-115763RB-I00 to JMSS from Spanish MICINN. RMM is recipient of a postdoctoral contract (2018/023) from the CDEIGENT program of Generalitat Valenciana. ACS is recipient of a predoctoral contract (FPU17/00715) from the Spanish Ministerio de Educacion. es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof Plant Growth Regulation es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Androgenesis es_ES
dc.subject Hormones es_ES
dc.subject Microspore culture es_ES
dc.subject Microspore embryogenesis es_ES
dc.subject Solanum melongena es_ES
dc.subject.classification GENETICA es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Quantitative and qualitative study of endogenous and exogenous growth regulators in eggplant (Solanum melongena) microspore cultures es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10725-021-00780-y es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115763RB-I00/ES/ESTUDIO DEL PAPEL DEL CALCIO EN LA INDUCCION DE EMBRIOGENESIS IN VITRO EN ESPECIES VEGETALES MODELO Y RECALCITRANTES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MECD//FPU17%2F00715/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/CIUCSD//CDEIGENT%2F2018%2F023/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Calabuig-Serna, A.; Camacho-Fernández, C.; Mir Moreno, R.; Porcel, R.; Carrera, E.; Lopez Diaz, I.; Seguí-Simarro, JM. (2022). Quantitative and qualitative study of endogenous and exogenous growth regulators in eggplant (Solanum melongena) microspore cultures. Plant Growth Regulation. 96(2):345-355. https://doi.org/10.1007/s10725-021-00780-y es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s10725-021-00780-y es_ES
dc.description.upvformatpinicio 345 es_ES
dc.description.upvformatpfin 355 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 96 es_ES
dc.description.issue 2 es_ES
dc.relation.pasarela S\451624 es_ES
dc.contributor.funder AGENCIA ESTATAL DE INVESTIGACION es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
dc.contributor.funder Ministerio de Educación, Cultura y Deporte es_ES
dc.contributor.funder Conselleria de Innovación, Universidades, Ciencia y Sociedad Digital, Generalitat Valenciana es_ES
dc.description.references Ayil-Gutiérrez B, Galaz-Ávalos RM, Peña-Cabrera E, Loyola-Vargas VM (2013) Dynamics of the concentration of IAA and some of its conjugates during the induction of somatic embryogenesis in Coffea canephora. Plant Signal Behav 8(11):e26998 es_ES
dc.description.references Calabuig-Serna A, Porcel R, Corral-Martínez P, Seguí-Simarro JM (2021) Anther and isolated microspore culture in eggplant (Solanum melongena L.). In: Seguí-Simarro JM (ed) Doubled haploid technology, vol 2: hot topics, apiaceae, brassicaceae, solanaceae. Methods in molecular biology, vol 2288, 1st edn. Humana Press, New York, pp 235–250 es_ES
dc.description.references Camacho-Fernández C, Hervás D, Rivas-Sendra A, Marín MP, Seguí-Simarro JM (2018) Comparison of six different methods to calculate cell densities. Plant Methods 14(1):30. https://doi.org/10.1186/s13007-018-0297-4 es_ES
dc.description.references Campion B, de Filippo G, Caporali E, Rossi G, Marziani G (1993) Endogenous growth regulator content in suspension cells of different male and female genotypes of asparagus. Plant Cell Tissue Organ Cult 33(3):281–285. https://doi.org/10.1007/bf02319013 es_ES
dc.description.references Centeno ML, Rodríguez A, Feito I, Fernández B (1996) Relationship between endogenous auxin and cytokinin levels and morphogenic responses in Actinidia deliciosa tissue cultures. Plant Cell Rep 16(1):58–62. https://doi.org/10.1007/bf01275450 es_ES
dc.description.references Cheng ZJ, Zhao XY, Shao XX, Wang F, Zhou C, Liu YG, Zhang Y, Zhang XS (2014) Abscisic acid regulates early seed development in Arabidopsis by ABI5-mediated transcription of SHORT HYPOCOTYL UNDER BLUE1. Plant Cell 26(3):1053–1068. https://doi.org/10.1105/tpc.113.121566 es_ES
dc.description.references Corral-Martínez P, Seguí-Simarro JM (2012) Efficient production of callus-derived doubled haploids through isolated microspore culture in eggplant (Solanum melongena L.). Euphytica 187(1):47–61. https://doi.org/10.1007/s10681-012-0715-z es_ES
dc.description.references Corral-Martínez P, Seguí-Simarro JM (2014) Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins. Euphytica 195(3):369–382. https://doi.org/10.1007/s10681-013-1001-4 es_ES
dc.description.references Corral-Martínez P, Camacho-Fernández C, Mir R, Seguí-Simarro JM (2021) Doubled haploid production in high- and low-response genotypes of rapeseed (Brassica napus) through isolated microspore culture. In: Seguí-Simarro JM (ed) Doubled haploid technology, vol 2: hot topics, apiaceae, brassicaceae, solanaceae. Methods in molecular biology, vol 2288, 1st edn. Humana Press, New York, pp 129–144 es_ES
dc.description.references Hays DB, Mandel RM, Pharis RP (2001) Hormones in zygotic and microspore embryos of Brassica napus. Plant Growth Regul 35(1):47–58 es_ES
dc.description.references Jouannic S, Champion A, Seguí-Simarro JM, Salimova E, Picaud A, Tregear J, Testillano P, Risueno MC, Simanis V, Kreis M, Henry Y (2001) The protein kinases AtMAP3Kε1 and BnMAP3Kε1 are functional homologues of S. pombe cdc7p and may be involved in cell division. Plant J 26(6):637–649 es_ES
dc.description.references Li H, Soriano M, Cordewener J, Muiño JM, Riksen T, Fukuoka H, Angenent GC, Boutilier K (2014) The histone deacetylase inhibitor Trichostatin A promotes totipotency in the male gametophyte. Plant Cell 26(1):195–209. https://doi.org/10.1105/tpc.113.116491 es_ES
dc.description.references Liu Z-H, Hsiao I-C, Pan Y-W (1996) Effect of naphthaleneacetic acid on endogenous indole-3-acetic acid, peroxidase and auxin oxidase in hypocotyl cuttings of soybean during root formation. Bot Bull Acad Sin 37(4):247–253 es_ES
dc.description.references Miyoshi K (1996) Callus induction and plantlet formation through culture of isolated microspores of eggplant (Solanum melongena L.). Plant Cell Rep 15(6):391–395 es_ES
dc.description.references Pal AK, Acharya K, Ahuja PS (2012) Endogenous auxin level is a critical determinant for in vitro adventitious shoot regeneration in potato (Solanum tuberosum L.). J Plant Biochem Biotechnol 21(2):205–212. https://doi.org/10.1007/s13562-011-0092-z es_ES
dc.description.references Parra-Vega V, Renau-Morata B, Sifres A, Seguí-Simarro JM (2013) Stress treatments and in vitro culture conditions influence microspore embryogenesis and growth of callus from anther walls of sweet pepper (Capsicum annuum L.). Plant Cell Tissue Organ Cult 112(3):353–360. https://doi.org/10.1007/s11240-012-0242-6 es_ES
dc.description.references Pescador R, Kerbauy GB, de Melo FW, Purgatto E, Suzuki RM, Guerra MP (2012) A hormonal misunderstanding in Acca sellowiana embryogenesis: levels of zygotic embryogenesis do not match those of somatic embryogenesis. Plant Growth Regul 68(1):67–76. https://doi.org/10.1007/s10725-012-9694-2 es_ES
dc.description.references Ramesar-Fortner NS, Yeung EC (2006) Physiological influences in the development and function of the shoot apical meristem of microspore-derived embryos of Brassica napus “Topas.” Can J Bot 84(3):371–383 es_ES
dc.description.references Rivas-San Vicente M, Plasencia J (2011) Salicylic acid beyond defence: its role in plant growth and development. J Exp Bot 62(10):3321–3338. https://doi.org/10.1093/jxb/err031 es_ES
dc.description.references Rivas-Sendra A, Campos-Vega M, Calabuig-Serna A, Seguí-Simarro JM (2017) Development and characterization of an eggplant (Solanum melongena) doubled haploid population and a doubled haploid line with high androgenic response. Euphytica 213(4):89. https://doi.org/10.1007/s10681-017-1879-3 es_ES
dc.description.references Rivas-Sendra A, Corral-Martínez P, Camacho-Fernández C, Porcel R, Seguí-Simarro JM (2020) Effects of growth conditions of donor plants and in vitro culture environment in the viability and the embryogenic response of microspores of different eggplant genotypes. Euphytica 216(11):167. https://doi.org/10.1007/s10681-020-02709-4 es_ES
dc.description.references Salas P, Prohens J, Seguí-Simarro JM (2011) Evaluation of androgenic competence through anther culture in common eggplant and related species. Euphytica 182(2):261–274. https://doi.org/10.1007/s10681-011-0490-2 es_ES
dc.description.references Salas P, Rivas-Sendra A, Prohens J, Seguí-Simarro JM (2012) Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures. Euphytica 184(2):235–250. https://doi.org/10.1007/s10681-011-0569-9 es_ES
dc.description.references Satpute G, Long H, Seguí-Simarro JM, Risueño MC, Testillano PS (2005) Cell architecture during gametophytic and embryogenic microspore development in Brassica napus. Acta Physiol Plant 27(4B):665–674 es_ES
dc.description.references Seguí-Simarro JM (2016) Androgenesis in solanaceae. In: Germanà MA, Lambardi M (eds) In vitro embryogenesis. Methods in molecular biology, vol 1359. Springer, New York, pp 209–244 es_ES
dc.description.references Seguí-Simarro JM, Corral-Martínez P, Parra-Vega V, González-García B (2011) Androgenesis in recalcitrant solanaceous crops. Plant Cell Rep 30(5):765–778. https://doi.org/10.1007/s00299-010-0984-8 es_ES
dc.description.references Seguí-Simarro JM, Jacquier NMA, Widiez T (2021) Overview of in vitro and in vivo doubled haploid technologies. In: Seguí-Simarro JM (ed) Doubled haploid technology, vol 1: general topics, alliaceae, cereals. Methods in molecular biology, vol 2287, 1st edn. Humana Press, New York, pp 3–22 es_ES
dc.description.references Seo M, Jikumaru Y, Kamiya Y (2011) Profiling of hormones and related metabolites in seed dormancy and germination studies. In: Kermode AR (ed) Seed dormancy: methods and protocols. Humana Press, Totowa, pp 99–111 es_ES
dc.description.references Touraev A, Heberle-Bors E (2003) Anther and microspore culture in tobacco. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. Kluwer Academic Publishers, Dordrecht, pp 223–228 es_ES
dc.description.references van Bergen S, Kottenhagen MJ, van der Meulen RM, Wang M (1999) The role of abscisic acid in induction of androgenesis: a comparative study between Hordeum vulgare L-cvs. Igri and Digger. J Plant Growth Regul 18(3):135–143 es_ES
dc.description.references Vergne P, Gaillard A (2021) Isolation of staged and viable maize microspores for DH production. In: Seguí-Simarro JM (ed) Doubled haploid technology, vol 1: general topics, alliaceae, cereals. Methods in molecular biology, vol 2287, 1st edn. Humana Press, New York, pp 281–293 es_ES
dc.description.references Vondráková Z, Eliášová K, Fischerová L, Vágner M (2011) The role of auxins in somatic embryogenesis of Abies alba. Cent Eur J Biol 6(4):587–596. https://doi.org/10.2478/s11535-011-0035-7 es_ES
dc.description.references Weyen J (2021) Applications of doubled haploids in plant breeding and applied research. In: Seguí-Simarro JM (ed) Doubled haploid technology, vol 1: general topics, alliaceae, cereals. Methods in molecular biology, vol 2287, 1st edn. Humana Press, New York, pp 23–39 es_ES
dc.description.references Yan SP, Yang RH, Wang F, Sun LN, Song XS (2017) Effect of auxins and associated metabolic changes on cuttings of hybrid aspen. Forests 8(4):117 es_ES
dc.description.references Żur I, Dubas E, Krzewska M, Janowiak F (2015) Current insights into hormonal regulation of microspore embryogenesis. Front Plant Sci 6:424. https://doi.org/10.3389/fpls.2015.00424 es_ES


Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem