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A cellular analysis of meristem activity at the end of flowering points to cytokinin as a major regulator of proliferative arrest in Arabidopsis

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A cellular analysis of meristem activity at the end of flowering points to cytokinin as a major regulator of proliferative arrest in Arabidopsis

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dc.contributor.author MERELO CREMADES, PAZ es_ES
dc.contributor.author González-Cuadra, Irene es_ES
dc.contributor.author FERRANDIZ MAESTRE, CRISTINA es_ES
dc.date.accessioned 2023-10-10T18:02:26Z
dc.date.available 2023-10-10T18:02:26Z
dc.date.issued 2022-02-28 es_ES
dc.identifier.issn 0960-9822 es_ES
dc.identifier.uri http://hdl.handle.net/10251/197955
dc.description.abstract [EN] In monocarpic plants, all reproductive meristem activity arrests and flower production ceases after the production of a certain number of fruits. This proliferative arrest (PA) is an evolutionary adaptation that ensures nutrient availability for seed production. Moreover, PA is a process of agronomic interest because it affects the duration of the flowering period and therefore fruit production. While our knowledge of the inputs and genetic factors controlling the initiation of the flowering period is extensive, little is known about the regulatory pathways and cellular events that participate in the end of flowering and trigger PA. Here, we characterize with high spatiotemporal resolution the cellular and molecular changes related to cell proliferation and meristem activity in the shoot apical meristem throughout the flowering period and PA. Our results suggest that cytokinin (CK) signaling repression precedes PA and that this hormone is sufficient to prevent and revert the process. We have also observed that repression of known CK downstream factors, such as type B cyclins and WUSCHEL (WUS), correlates with PA. These molecular changes are accompanied by changes in cell size and number likely caused by the cessation of cell division and WUS activity during PA. Parallel assays in fruitfull (ful) mutants, which do not undergo PA, have revealed that FUL may promote PA via repression of these CK-dependent pathways. Moreover, our data allow to define two phases, based on the relative contribution of FUL, that lead to PA: an early reduction of CK-related events and a late blocking of these events. es_ES
dc.description.sponsorship We thank Bruno Mudller and Venugopala Reddy for kindly providing the TCSn:GFP-ER and WUSpro:EGFP-WUS lines, respectively, as well as Vicente Balanza, Neha Bhatia, Antonio Serrano-Mislata, Concha Gomez-Mena, and Francisco Madueo for helpful feedback on the manuscript. P.M. acknowledges Fundacion General CSIC (ComFuturo program) for current funding. The laboratory of C.F. is supported by grants from Ministerio de Ciencia e In-novacion (RTI2018-099239-B-I00) and Generalitat Valenciana (PROMETEU/2019/004) . es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Current Biology es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Arabidopsis thaliana es_ES
dc.subject Shoot apical meristem es_ES
dc.subject Proliferative arrest es_ES
dc.subject Cytokinin es_ES
dc.subject Meristem size es_ES
dc.subject Cell division es_ES
dc.subject WUSCHEL es_ES
dc.subject FRUITFULL es_ES
dc.title A cellular analysis of meristem activity at the end of flowering points to cytokinin as a major regulator of proliferative arrest in Arabidopsis es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.cub.2021.11.069 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099239-B-I00/ES/HACIA UN MODELO INTEGRADO DE LA PARADA PROLIFERTIVA DE LOS MERISTEMOS INFLORESCENTES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Generalitat Valenciana//PROMETEO%2F2019%2F004//NON-STOP CROP. Identificación de factores que influyen en el rendimiento de los cultivos mediante el control de la actividad meristemática. / es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/101034263/EU es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI//RTI2018-099239-B-I00//HACIA UN MODELO INTEGRADO DE LA PARADA PROLIFERTIVA DE LOS MERISTEMOS INFLORESCENTES/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Merelo Cremades, P.; González-Cuadra, I.; Ferrandiz Maestre, C. (2022). A cellular analysis of meristem activity at the end of flowering points to cytokinin as a major regulator of proliferative arrest in Arabidopsis. Current Biology. 32(4):749-762. https://doi.org/10.1016/j.cub.2021.11.069 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.cub.2021.11.069 es_ES
dc.description.upvformatpinicio 749 es_ES
dc.description.upvformatpfin 762 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 32 es_ES
dc.description.issue 4 es_ES
dc.identifier.pmid 34963064 es_ES
dc.relation.pasarela S\456143 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Consejo Superior de Investigaciones Científicas es_ES


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