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dc.contributor.author | Vives Hernandez, Karel | es_ES |
dc.contributor.author | Moreno-Romero, Jordi | es_ES |
dc.contributor.author | Hernandez de la Torre, Martha | es_ES |
dc.contributor.author | Pérez Manríquez, Claudia | es_ES |
dc.contributor.author | Rios Leal, Darcy | es_ES |
dc.contributor.author | Martinez-Garcia, Jaime F. | es_ES |
dc.date.accessioned | 2023-11-08T19:01:09Z | |
dc.date.available | 2023-11-08T19:01:09Z | |
dc.date.issued | 2022-02 | es_ES |
dc.identifier.issn | 0031-9422 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/199459 | |
dc.description.abstract | [EN] Stevia rebaudiana leaf extracts contain stevioside and rebaudioside A, two steviol glycosides (SGs) used as natural sweeteners because of their non-toxic, thermally stable and non-caloric properties. Indeed, leaf extracts can be up to 300 times sweeter than sucrose. Stevioside and rebaudioside A have organoleptic differences, the first one having an undesirable bitterness and the second one a higher sweetener capacity. Selection of the S. rebaudiana varieties and the best environmental conditions that elicit higher SGs content and the appropriate composition is an important goal. In this study we quantified and compared the amount of stevioside and rebaudioside A in two of the most used S. rebaudiana cultivars, Morita II and Criolla. Our results show a strong differential ratio of stevioside and rebaudioside A accumulated in the leaf between these cultivars. The Criolla cultivar showed about 3 times more stevioside per mg of dry weight than Morita II, whereas the Morita II accumulated almost 10 times more rebaudioside A than that produced in Criolla. We observed an enhanced expression in Morita II of three genes (SrKA13H, SrUGT74G1 and SrUGT76G1) known to encode three enzymes that participate in SGs biosynthesis, likely contributing to the differences in the stevioside and rebaudioside A accumulation. Not only genetic variation can affect SGs composition, but also environmental factors and crop management. Numerous studies have shown that the light regime in which S. rebaudiana cultivars grow can affect SGs accumulation. However, the optimal light regime to increase total SGs content is currently controversial. By applying various light intensities, we detected an increase of expression of these three biosynthetic genes at higher light intensity, accompanied by higher levels of stevioside and rebaudioside A, demonstrating that light intensity influences the synthesis of SGs. | es_ES |
dc.description.sponsorship | This work was supported by the European Commission [H2020- MSCA-IF-2017, grant number 79747] to JM-R; research in CRAG was supported by the Spanish MINECO-FEDER [grant number BIO2017- 85316-R] and AGAUR [grant number 2017-SGR1211 and Xarba] to JFM-G; the support of MINECO for the Centro de Excelencia Severo Ochoa 2016-2019 [grant number SEV-2015-0533]; and by the CERCA Program of the Generalitat de Catalunya. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Phytochemistry | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Stevia rebaudiana | es_ES |
dc.subject | Asteraceae | es_ES |
dc.subject | Quantification and gene expression | es_ES |
dc.subject | Steviol glycosides | es_ES |
dc.subject | Stevioside | es_ES |
dc.subject | Rebaudioside A | es_ES |
dc.subject | SrKA13H | es_ES |
dc.subject | SrUGT74G1 | es_ES |
dc.subject | SrUGT76G1 | es_ES |
dc.title | Effect of light intensity on steviol glycosides production in leaves of Stevia rebaudiana plants | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.phytochem.2021.113027 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-85316-R/ES/NUEVAS FRONTERAS EN LA COMPRENSION DE LAS RESPUESTAS A LA SOMBRA VEGETAL: DE PLANTULAS A SEMILLAS Y DE MODELOS A CULTIVOS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AGAUR//2017-SGR1211/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//SEV-2015-0533/ES/AGR-CONSORCI CSIC-IRTA-UAB CENTRE DE RECERCA EN AGRIGENOMICA (CRAG)/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.description.bibliographicCitation | Vives Hernandez, K.; Moreno-Romero, J.; Hernandez De La Torre, M.; Pérez Manríquez, C.; Rios Leal, D.; Martinez-Garcia, JF. (2022). Effect of light intensity on steviol glycosides production in leaves of Stevia rebaudiana plants. Phytochemistry. 194:1-10. https://doi.org/10.1016/j.phytochem.2021.113027 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.phytochem.2021.113027 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 10 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 194 | es_ES |
dc.identifier.pmid | 34861537 | es_ES |
dc.relation.pasarela | S\460342 | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.contributor.funder | Generalitat de Catalunya | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |
dc.contributor.funder | Agencia de Gestión de Ayudas Universitarias y de Investigación | es_ES |