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Effect of light intensity on steviol glycosides production in leaves of Stevia rebaudiana plants

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Effect of light intensity on steviol glycosides production in leaves of Stevia rebaudiana plants

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dc.contributor.author Vives Hernandez, Karel es_ES
dc.contributor.author Moreno-Romero, Jordi es_ES
dc.contributor.author Hernandez de la Torre, Martha es_ES
dc.contributor.author Pérez Manríquez, Claudia es_ES
dc.contributor.author Rios Leal, Darcy es_ES
dc.contributor.author Martinez-Garcia, Jaime F. es_ES
dc.date.accessioned 2023-11-08T19:01:09Z
dc.date.available 2023-11-08T19:01:09Z
dc.date.issued 2022-02 es_ES
dc.identifier.issn 0031-9422 es_ES
dc.identifier.uri http://hdl.handle.net/10251/199459
dc.description.abstract [EN] Stevia rebaudiana leaf extracts contain stevioside and rebaudioside A, two steviol glycosides (SGs) used as natural sweeteners because of their non-toxic, thermally stable and non-caloric properties. Indeed, leaf extracts can be up to 300 times sweeter than sucrose. Stevioside and rebaudioside A have organoleptic differences, the first one having an undesirable bitterness and the second one a higher sweetener capacity. Selection of the S. rebaudiana varieties and the best environmental conditions that elicit higher SGs content and the appropriate composition is an important goal. In this study we quantified and compared the amount of stevioside and rebaudioside A in two of the most used S. rebaudiana cultivars, Morita II and Criolla. Our results show a strong differential ratio of stevioside and rebaudioside A accumulated in the leaf between these cultivars. The Criolla cultivar showed about 3 times more stevioside per mg of dry weight than Morita II, whereas the Morita II accumulated almost 10 times more rebaudioside A than that produced in Criolla. We observed an enhanced expression in Morita II of three genes (SrKA13H, SrUGT74G1 and SrUGT76G1) known to encode three enzymes that participate in SGs biosynthesis, likely contributing to the differences in the stevioside and rebaudioside A accumulation. Not only genetic variation can affect SGs composition, but also environmental factors and crop management. Numerous studies have shown that the light regime in which S. rebaudiana cultivars grow can affect SGs accumulation. However, the optimal light regime to increase total SGs content is currently controversial. By applying various light intensities, we detected an increase of expression of these three biosynthetic genes at higher light intensity, accompanied by higher levels of stevioside and rebaudioside A, demonstrating that light intensity influences the synthesis of SGs. es_ES
dc.description.sponsorship This work was supported by the European Commission [H2020- MSCA-IF-2017, grant number 79747] to JM-R; research in CRAG was supported by the Spanish MINECO-FEDER [grant number BIO2017- 85316-R] and AGAUR [grant number 2017-SGR1211 and Xarba] to JFM-G; the support of MINECO for the Centro de Excelencia Severo Ochoa 2016-2019 [grant number SEV-2015-0533]; and by the CERCA Program of the Generalitat de Catalunya. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Phytochemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Stevia rebaudiana es_ES
dc.subject Asteraceae es_ES
dc.subject Quantification and gene expression es_ES
dc.subject Steviol glycosides es_ES
dc.subject Stevioside es_ES
dc.subject Rebaudioside A es_ES
dc.subject SrKA13H es_ES
dc.subject SrUGT74G1 es_ES
dc.subject SrUGT76G1 es_ES
dc.title Effect of light intensity on steviol glycosides production in leaves of Stevia rebaudiana plants es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.phytochem.2021.113027 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-85316-R/ES/NUEVAS FRONTERAS EN LA COMPRENSION DE LAS RESPUESTAS A LA SOMBRA VEGETAL: DE PLANTULAS A SEMILLAS Y DE MODELOS A CULTIVOS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AGAUR//2017-SGR1211/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//SEV-2015-0533/ES/AGR-CONSORCI CSIC-IRTA-UAB CENTRE DE RECERCA EN AGRIGENOMICA (CRAG)/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.description.bibliographicCitation Vives Hernandez, K.; Moreno-Romero, J.; Hernandez De La Torre, M.; Pérez Manríquez, C.; Rios Leal, D.; Martinez-Garcia, JF. (2022). Effect of light intensity on steviol glycosides production in leaves of Stevia rebaudiana plants. Phytochemistry. 194:1-10. https://doi.org/10.1016/j.phytochem.2021.113027 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.phytochem.2021.113027 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 10 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 194 es_ES
dc.identifier.pmid 34861537 es_ES
dc.relation.pasarela S\460342 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Generalitat de Catalunya es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Agencia de Gestión de Ayudas Universitarias y de Investigación es_ES


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