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A refined method for ovule culture in sugar beet (Beta vulgaris L.)

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A refined method for ovule culture in sugar beet (Beta vulgaris L.)

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dc.contributor.author Sohrabi, Sara es_ES
dc.contributor.author Abdollahi, Mohammad Reza es_ES
dc.contributor.author Mirzaie-Asl, Asghar es_ES
dc.contributor.author Koulaei, Hassan Ebrahimi es_ES
dc.contributor.author Aghaeezadeh, Mohsen es_ES
dc.contributor.author Seguí-Simarro, Jose M. es_ES
dc.date.accessioned 2023-11-08T19:01:30Z
dc.date.available 2023-11-08T19:01:30Z
dc.date.issued 2021-08 es_ES
dc.identifier.issn 0167-6857 es_ES
dc.identifier.uri http://hdl.handle.net/10251/199461
dc.description.abstract [EN] Induction of gynogenesis through ovule culture is a valuable tool to produce haploid and doubled haploid plants in sugar beet (Beta vulgaris L.). However, there is still large room for refining the method. In this study we investigated the gynogenic response of cultured ovules of three sugar beet genotypes, the effect of the application to inflorescences of different pretreatments with mannitol at 4oC and with 5-azacytidine and 2,4-D, and the effect of the use of different basal culture media and sucrose concentrations. The response was evaluated in terms of percentages of induction of gynogenesis, embryogenesis and callogenesis, as well as of regenerated plants. We showed that a pretreatment with 0.5 M mannitol at 4 degrees C for 4 days, and with 50 mu M 5-AzaC for 1 h, notably improved the percentage of embryogenesis and plant regeneration. Besides, the use of MS basal medium and 60 g/L sucrose was also found beneficial. This study provides new ways to improve the efficiency of haploid induction and plant regeneration through ovule culture in sugar beet, and is potentially applicable to ovule culture in other crops. Key message This study provides new ways to improve the efficiency of haploid induction and plant regeneration through ovule culture in sugar beet. es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof Plant Cell Tissue and Organ Culture (PCTOC) es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject 2,4-D es_ES
dc.subject 5-azacytidine es_ES
dc.subject Doubled haploid es_ES
dc.subject Gynogenesis es_ES
dc.subject Haploid es_ES
dc.subject Mannitol es_ES
dc.subject Ovule culture es_ES
dc.subject.classification GENETICA es_ES
dc.title A refined method for ovule culture in sugar beet (Beta vulgaris L.) es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s11240-021-02065-8 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Sohrabi, S.; Abdollahi, MR.; Mirzaie-Asl, A.; Koulaei, HE.; Aghaeezadeh, M.; Seguí-Simarro, JM. (2021). A refined method for ovule culture in sugar beet (Beta vulgaris L.). Plant Cell Tissue and Organ Culture (PCTOC). 146(2):259-267. https://doi.org/10.1007/s11240-021-02065-8 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s11240-021-02065-8 es_ES
dc.description.upvformatpinicio 259 es_ES
dc.description.upvformatpfin 267 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 146 es_ES
dc.description.issue 2 es_ES
dc.relation.pasarela S\443138 es_ES
dc.description.references Abdollahi MR, Rashidi S (2018) Production and conversion of haploid embryos in chickpea (Cicer arietinum L.) anther cultures using high 2, 4-D and silver nitrate containing media. Plant Cell Tiss Organ Cult 133:39–49 es_ES
dc.description.references Aflaki F, Pazuki A, Gurel S, Stevanato P, Biancardi E, Gurel E (2017) Doubled haploid sugar beet: an integrated view of factors influencing the processes of gynogenesis and chromosome doubling. International Sugar Journal 119:884–895 es_ES
dc.description.references Ardebili SH, Shariatpanahi ME, Amiri R, Emamifar M, Oroojloo M, Nematzadeh G, Sadat Noori SA, Heberle-Bors E (2011) Effect of 2, 4-D as a Novel Inducer of Embryogenesis in Microspores of Brassica napus L. Czech J Genet Plant Breed 47:114–122 es_ES
dc.description.references Barański R (1996) In vitro gynogenesis in red beet (Beta vulgaris L.): effects of ovule culture conditions. Acta Soc Bot Pol 65:57–60 es_ES
dc.description.references Bohanec B (2009) Doubled Haploids via Gynogenesis. In: Touraev AF, Forster BP, Jain SM (eds) Advances in Haploid Production in Higher Plants. Springer, pp 35–46 es_ES
dc.description.references Chen JF, Cui L, Malik AA, Mbira KG (2011) In vitro haploid and dihaploid production via unfertilized ovule culture. Plant Cell Tiss Organ Cult 104:311–319 es_ES
dc.description.references Corral-Martínez P, Seguí-Simarro JM (2012) Efficient production of callus-derived doubled haploids through isolated microspore culture in eggplant (Solanum melongena L.). Euphytica 187(1):47–61 es_ES
dc.description.references Corral-Martínez P, Seguí-Simarro JM (2014) Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins. Euphytica 195(3):369–382 es_ES
dc.description.references De Greef W, Jacobs M (1979) In vitro culture of the sugarbeet: description of a cell line with high regeneration capacity. Plant Sci Lett 17:55–61 es_ES
dc.description.references Fraga HP, Vieira LN, Caprestano CA, Steinmacher DA, Micke GA, Spudeit DA, Pescador R, Guerra MP (2012) 5-Azacytidine combined with 2,4-D improves somatic embryogenesis of Acca sellowiana (O. Berg) Burret by means of changes in global DNA methylation levels. Plant Cell Rep 31:2165–2176 es_ES
dc.description.references Grzybkowska D, Morończyk J, Wójcikowska B, Gaj MD (2018) Azacitidine (5-AzaC)-treatment and mutations in DNA methylase genes affect embryogenic response and expression of the genes that are involved in somatic embryogenesis in Arabidopsis. Plant Growth Regul 85:243–256 es_ES
dc.description.references Gürel S, Gürel E, Kaya Z (2000) Doubled haploid plant production from unpollinated ovules of sugar beet (Beta vulgaris L.). Plant Cell Rep 19:1155–1159 es_ES
dc.description.references Gürel S, Gürel E, Kaya Z, Erdal M, Güler E (2003) Effects of antimitotic agents on haploid plant production from unpollinated ovules of sugar beet (Beta vulgaris L.). Biotechnol Biotechnol Equip 17:97–101 es_ES
dc.description.references Hassani M, Heidari B, Dadkhodaie A, Stevanato P (2018) Genotype by environment interaction components underlying variations in root, sugar and white sugar yield in sugar beet (Beta vulgaris L.). Euphytica 214:79 es_ES
dc.description.references Klimek-Chodacka M, Baranski R (2013) Comparison of haploid and doubled haploid sugar beet clones in their ability to micropropagate and regenerate. Electron J Biotechnol 16:1–10 es_ES
dc.description.references Leljak-Levanić D, Bauer N, Mihaljević S, Jelaska S (2004) Changes in DNA methylation during somatic embryogenesis in Cucurbita pepo L. Plant Cell Rep 23:120–127 es_ES
dc.description.references Li H, Soriano M, Cordewener J, Muiño JM, Riksen T, Fukuoka H, Angenent GC, Boutilier K (2014) The histone deacetylase inhibitor Trichostatin A promotes totipotency in the male gametophyte. Plant Cell 26(1):195–209 es_ES
dc.description.references Lipavska H, Vreugdenhil D (1996) Uptake of mannitol from the media by in vitro grown plants. Plant Cell Tiss Organ Cult 45:103–107 es_ES
dc.description.references Lux H, Herrmann L, Wetzel C (1990) Production of haploid sugar beet (Beta vulgaris L.) by culturing unpollinated ovules. Plant Breed 104:177–183 es_ES
dc.description.references Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497 es_ES
dc.description.references Nagl N, Mezei S, Kovačev L, Vasić D, Čačić N (2004) Induction and micropropagation potential of sugar beet haploids. Genetika 36:187–194 es_ES
dc.description.references Nowaczyk L, Nowaczyk P, Olszewska D, Niklas-Nowak A (2015) Effect of 2,4-dichlorophenoxyacetic acid pretreatment of Capsicum spp. donor plants on the anther culture efficiency of lines selected by capsaicinoid content. Biotechnologia 96:179–183 es_ES
dc.description.references Osorio-Montalvo P, Sáenz-Carbonell L, De-la-Peña C (2018) 5-Azacytidine: a promoter of epigenetic changes in the quest to improve plant somatic embryogenesis. Int J Mol Sci 19:3182 es_ES
dc.description.references Parra-Vega V, Renau-Morata B, Sifres A, Seguí-Simarro JM (2013) Stress treatments and in vitro culture conditions influence microspore embryogenesis and growth of callus from anther walls of sweet pepper (Capsicum annuum L.). Plant Cell Tissue Organ Cult 112(3):353–360 es_ES
dc.description.references Pazuki A, Aflaki F, Gürel E, Ergül A, Gürel S (2017) A robust method for haploid sugar beet in vitro proliferation and hyperhydricity reduction. Folia Hortic 29:241–250 es_ES
dc.description.references Pazuki A, Aflaki F, Gürel E, Ergül A, Gürel S (2018a) Gynogenesis induction in sugar beet (Beta vulgaris) improved by 6-benzylaminopurine (BAP) and synergized with cold pretreatment. Sugar Tech 20:69–77 es_ES
dc.description.references Pazuki A, Aflaki F, Gürel S, Ergül A, Gürel E (2018b) Production of doubled haploids in sugar beet (Beta vulgaris): an efficient method by a multivariate experiment. Plant Cell Tiss Organ Cult 132:85–97 es_ES
dc.description.references Pazuki A, Aflaki F, Gurel S, Ergul A, Gurel E (2018c) The effects of proline on in vitro proliferation and propagation of doubled haploid sugar beet (Beta vulgaris). Turk J Bot 42:280–288 es_ES
dc.description.references Salas P, Prohens J, Seguí-Simarro JM (2011) Evaluation of androgenic competence through anther culture in common eggplant and related species. Euphytica 182(2):261–274 es_ES
dc.description.references Seguí-Simarro JM (2016) Androgenesis in solanaceae. In: Germanà MA, Lambardi M (eds) In vitro embryogenesis, vol 1359. Methods in Molecular Biology. Springer Science + Business Media, New York, pp 209–244 es_ES
dc.description.references Shariatpanahi ME, Bal U, Heberle-Bors E, Touraev A (2006) Stresses applied for the re-programming of plant microspores towards in vitro embryogenesis. Physiol Plant 127:519–534 es_ES
dc.description.references Testillano PS (2019) Microspore embryogenesis: targeting the determinant factors of stress-induced cell reprogramming for crop improvement. J Exp Bot 70(11):2965–2978 es_ES
dc.description.references Tomaszewska-Sowa M (2010) Cytometric analyses of sugar beet (Beta vulgaris L.) plants regenerated from unfertilized ovules cultured in vitro. Electron J Pol Agric Univ 13:1–10 es_ES
dc.description.references Yamamoto N, Kobayashi H, Togashi T, Mori Y, Kikuchi K, Kuriyama K, Tokuji Y (2005) Formation of embryogenic cell clumps from carrot epidermal cells is suppressed by 5-azacytidine, a DNA methylation inhibitor. J Plant Physiol 162:47–54 es_ES
dc.description.references Yaseen M, Ahmad T, Sablok G, Standardi A, Hafiz IA (2013) Role of carbon sources for in vitro plant growth and development. Mol Biol Rep 40:2837–2849 es_ES


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