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CryoEM structures of Arabidopsis DDR complexes involved in RNA-directed DNA methylation

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CryoEM structures of Arabidopsis DDR complexes involved in RNA-directed DNA methylation

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dc.contributor.author Wongpalee, Somsakul Pop es_ES
dc.contributor.author Liu, Shiheng es_ES
dc.contributor.author Gallego Bartolomé, Javier es_ES
dc.contributor.author Leitner, Alexander es_ES
dc.contributor.author Aebersold, Ruedi es_ES
dc.contributor.author Liu, Wanlu es_ES
dc.contributor.author Yen, Linda es_ES
dc.contributor.author Nohales, Maria A. es_ES
dc.contributor.author Kuo, Peggy Hsuanyu es_ES
dc.contributor.author Vashisht, Ajay A. es_ES
dc.contributor.author Wohlschlegel, James A. es_ES
dc.contributor.author Feng, Suhua es_ES
dc.contributor.author Kay, Steve A. es_ES
dc.contributor.author Zhou, Z. Hong es_ES
dc.contributor.author Jacobsen, Steven E. es_ES
dc.date.accessioned 2023-12-21T19:02:29Z
dc.date.available 2023-12-21T19:02:29Z
dc.date.issued 2019-09-02 es_ES
dc.identifier.issn 2041-1723 es_ES
dc.identifier.uri http://hdl.handle.net/10251/201054
dc.description.abstract [EN] Transcription by RNA polymerase V (Pol V) in plants is required for RNA-directed DNA methylation, leading to transcriptional gene silencing. Global chromatin association of Pol V requires components of the DDR complex DRD1, DMS3 and RDM1, but the assembly process of this complex and the underlying mechanism for Pol V recruitment remain unknown. Here we show that all DDR complex components co-localize with Pol V, and we report the cryoEM structures of two complexes associated with Pol V recruitment-DR (DMS3-RDM1) and DDR' (DMS3-RDM1-DRD1 peptide), at 3.6 angstrom and 3.5 angstrom resolution, respectively. RDM1 dimerization at the center frames the assembly of the entire complex and mediates interactions between DMS3 and DRD1 with a stoichiometry of 1 DRD1:4 DMS3:2 RDM1. DRD1 binding to the DR complex induces a drastic movement of a DMS3 coiled-coil helix bundle. We hypothesize that both complexes are functional intermediates that mediate Pol V recruitment. es_ES
dc.description.sponsorship This research was supported by in part by grants from the National Institutes of Health (1R35GM130272 to S.E.J. and R01GM071940/AI094386/DE025567/DE028583 to Z.H.Z.), a Bill and Melinda Gates Foundation grant (OPP1125410) to S.E.J., and the European Research Council (ERC AdG 679821 to R.A.). We acknowledge the cryoEM resource in the Electron Imaging Center for Nanomachines supported by UCLA and grants from NIH (S10RR23057, S10OD018111, and U24GM116792) and NSF (DMR-1548924 and DBI-1338135). We thank Xiaojun Wang, Douglas Black, and Michael Carey for providing bacterial expression plasmids, useful guidance in recombinant protein expression and allowing us to use laboratory equipment. es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Nature Communications es_ES
dc.rights Reconocimiento (by) es_ES
dc.title CryoEM structures of Arabidopsis DDR complexes involved in RNA-directed DNA methylation es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/s41467-019-11759-9 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NSF//DMR-1548924/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NSF//DBI-1338135/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ERC//679821/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIH//U24GM116792/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIH//1R35GM130272/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIH//R01GM071940%2FAI094386%2FDE025567%2FDE028583/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIH//S10RR23057/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIH//S10OD018111/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/BMGF//OPP1125410/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Wongpalee, SP.; Liu, S.; Gallego Bartolomé, J.; Leitner, A.; Aebersold, R.; Liu, W.; Yen, L.... (2019). CryoEM structures of Arabidopsis DDR complexes involved in RNA-directed DNA methylation. Nature Communications. 10:1-12. https://doi.org/10.1038/s41467-019-11759-9 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/s41467-019-11759-9 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 12 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 10 es_ES
dc.identifier.pmid 31477705 es_ES
dc.identifier.pmcid PMC6718625 es_ES
dc.relation.pasarela S\505606 es_ES
dc.contributor.funder European Research Council es_ES
dc.contributor.funder Bill and Melinda Gates Foundation es_ES
dc.contributor.funder National Science Foundation, EEUU es_ES
dc.contributor.funder National Institutes of Health, EEUU es_ES
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