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Ectopic targeting of CG DNA methylation in Arabidopsis with the bacterial SssI methyltransferase

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Ectopic targeting of CG DNA methylation in Arabidopsis with the bacterial SssI methyltransferase

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dc.contributor.author Liu, Wanlu es_ES
dc.contributor.author Gallego Bartolomé, Javier es_ES
dc.contributor.author Zhou, Yuxing es_ES
dc.contributor.author Zhong, Zhenhui es_ES
dc.contributor.author Wang, Ming es_ES
dc.contributor.author Wongpalee, Somsakul Pop es_ES
dc.contributor.author Gardiner, Jason es_ES
dc.contributor.author Feng, Suhua es_ES
dc.contributor.author Kuo, Peggy Hsuanyu es_ES
dc.contributor.author Jacobsen, Steven E. es_ES
dc.date.accessioned 2023-12-21T19:02:36Z
dc.date.available 2023-12-21T19:02:36Z
dc.date.issued 2021-05-25 es_ES
dc.identifier.issn 2041-1723 es_ES
dc.identifier.uri http://hdl.handle.net/10251/201056
dc.description.abstract [EN] The ability to target epigenetic marks like DNA methylation to specific loci is important in both basic research and in crop plant engineering. However, heritability of targeted DNA methylation, how it impacts gene expression, and which epigenetic features are required for proper establishment are mostly unknown. Here, we show that targeting the CG-specific methyltransferase M.SssI with an artificial zinc finger protein can establish heritable CG methylation and silencing of a targeted locus in Arabidopsis. In addition, we observe highly heritable widespread ectopic CG methylation mainly over euchromatic regions. This hypermethylation shows little effect on transcription while it triggers a mild but significant reduction in the accumulation of H2A.Z and H3K27me3. Moreover, ectopic methylation occurs preferentially at less open chromatin that lacks positive histone marks. These results outline general principles of the heritability and interaction of CG methylation with other epigenomic features that should help guide future efforts to engineer epigenomes. The ability to target DNA methylation to specific loci is important for both basic and applied research. Here, the authors fuse CG-specific methyltransferase SssI with an artificial zinc finger protein for DNA methylation targeting and show the chromatin features favorable for efficient gain of methylation. es_ES
dc.description.sponsorship We thank Mahnaz Akhavan for support with high-throughput sequencing at the University of California at Los Angeles (UCLA) Broad Stem Cell Research Center BioSequencing Core Facility. We also thank Life Science Editors (https://www.lifescienceeditors.com/) for editing assistance. J.G.B. was partially funded by the European Horizon 2020 Framework Programme (H2020-MSCA-IF-2018-835599) and the Spanish Ministry of Science, Innovation, and Universities (RYC2018-024108-I). W.L. was partially supported by the Fundamental Research Funds for the Central Universities (2021QN81016). This work was supported by NIH grant R35 GM130272, and by a Bill & Melinda Gates Foundation grant (OPP1210659) to S.E.J. S.E.J. is an Investigator of the Howard Hughes Medical Institute. es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Nature Communications es_ES
dc.rights Reconocimiento (by) es_ES
dc.title Ectopic targeting of CG DNA methylation in Arabidopsis with the bacterial SssI methyltransferase es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/s41467-021-23346-y es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/835599/EU es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIH//R35 GM130272/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Fundamental Research Funds for the Central Universities//2021QN81016/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//RYC2018-024108-I/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/BMGF//OPP1210659/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Liu, W.; Gallego Bartolomé, J.; Zhou, Y.; Zhong, Z.; Wang, M.; Wongpalee, SP.; Gardiner, J.... (2021). Ectopic targeting of CG DNA methylation in Arabidopsis with the bacterial SssI methyltransferase. Nature Communications. 12(1):1-14. https://doi.org/10.1038/s41467-021-23346-y es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/s41467-021-23346-y es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 14 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 12 es_ES
dc.description.issue 1 es_ES
dc.identifier.pmid 34035251 es_ES
dc.identifier.pmcid PMC8149686 es_ES
dc.relation.pasarela S\505585 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Bill and Melinda Gates Foundation es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder National Institutes of Health, EEUU es_ES
dc.contributor.funder Fundamental Research Funds for the Central Universities es_ES
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