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dc.contributor.author | Ausin, Israel![]() |
es_ES |
dc.contributor.author | Feng, Suhua![]() |
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dc.contributor.author | Yu, Chaowei![]() |
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dc.contributor.author | Liu, Wanlu![]() |
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dc.contributor.author | Kuo, Hsuan Yu![]() |
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dc.contributor.author | Jacobsen, Elise L.![]() |
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dc.contributor.author | Zhai, Jixian![]() |
es_ES |
dc.contributor.author | Gallego Bartolomé, Javier![]() |
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dc.contributor.author | Wang, Lin![]() |
es_ES |
dc.contributor.author | Egertsdotter, Ulrika![]() |
es_ES |
dc.contributor.author | Street, Nathaniel R.![]() |
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dc.contributor.author | Jacobsen, Steven E.![]() |
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dc.contributor.author | Wang, Haifeng![]() |
es_ES |
dc.date.accessioned | 2023-12-22T19:02:27Z | |
dc.date.available | 2023-12-22T19:02:27Z | |
dc.date.issued | 2016-12-13 | es_ES |
dc.identifier.issn | 0027-8424 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/201095 | |
dc.description.abstract | [EN] DNA methylation plays important roles in many biological processes, such as silencing of transposable elements, imprinting, and regulating gene expression. Many studies of DNA methylation have shown its essential roles in angiosperms (flowering plants). However, few studies have examined the roles and patterns of DNA methylation in gymnosperms. Here, we present genome-wide high coverage single-base resolution methylation maps of Norway spruce (Picea abies) from both needles and somatic embryogenesis culture cells via whole genome bisulfite sequencing. On average, DNA methylation levels of CG and CHG of Norway spruce were higher than most other plants studied. CHH methylation was found at a relatively low level; however, at least one copy of most of the RNA-directed DNA methylation pathway genes was found in Norway spruce, and CHH methylation was correlated with levels of siRNAs. In comparison with needles, somatic embryogenesis culture cells that are used for clonally propagating spruce trees showed lower levels of CG and CHG methylation but higher level of CHH methylation, suggesting that like in other species, these culture cells show abnormal methylation patterns. | es_ES |
dc.description.sponsorship | We thank members of the S.E.J. laboratory for useful discussions. We are grateful for support from the Umea Plant Science Centre (UPSC) bioinformatics platform. This work was supported by funds from National Natural Science Foundation of China Grant 31501031, Program for Excellent Youth Talents in Fujian Province University, and Fujian-Taiwan Joint Innovative Centre for Germplasm Resources and Cultivation of Crop (Fujian 2011 Program) (to H.W.), the University of California, Los Angeles-Fujian Agriculture and Forestry University Joint Research Center on Plant Proteomics, the Alice Wallenberg Foundation, the Swedish Research Council (VR), the Swedish Governmental Agency for Innovation Systems (Vinnova), the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (Formas), and the Swedish Foundation for Strategic Research, in part through the UPSC Berzelii Centre for Forest Biotechnology. W.L. is supported by a Philip J. Whitcome fellowship from the Molecular Biology Institute of University of California, Los Angeles and a scholarship from the Chinese Scholarship Council. J.Z. is a Life Science Research Foundation Postdoctoral Fellow, sponsored by the Gordon and Betty Moore Foundation. N.R.S. is supported by the Trees and Crops for the Future project. S.E.J. is an Investigator of the Howard Hughes Medical Institute. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Proceedings of the National Academy of Sciences | es_ES |
dc.relation.ispartof | Proceedings of the National Academy of Sciences | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | GymnospermsNorway spruce | es_ES |
dc.subject | DNA methylation | es_ES |
dc.subject | Small RNA | es_ES |
dc.subject | Genome size | es_ES |
dc.title | DNA methylome of the 20-gigabase Norway spruce genome. | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1073/pnas.1618019113 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/NSF//31501031/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Ausin, I.; Feng, S.; Yu, C.; Liu, W.; Kuo, HY.; Jacobsen, EL.; Zhai, J.... (2016). DNA methylome of the 20-gigabase Norway spruce genome. Proceedings of the National Academy of Sciences. 113(50):E8106-E8113. https://doi.org/10.1073/pnas.1618019113 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1073/pnas.1618019113 | es_ES |
dc.description.upvformatpinicio | E8106 | es_ES |
dc.description.upvformatpfin | E8113 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 113 | es_ES |
dc.description.issue | 50 | es_ES |
dc.identifier.pmid | 27911846 | es_ES |
dc.identifier.pmcid | PMC5167160 | es_ES |
dc.relation.pasarela | S\505592 | es_ES |
dc.contributor.funder | University of California | es_ES |
dc.contributor.funder | China Scholarship Council | es_ES |
dc.contributor.funder | Trees and Crops for the Future | es_ES |
dc.contributor.funder | Swedish Research Council Formas | es_ES |
dc.contributor.funder | Gordon and Betty Moore Foundation | es_ES |
dc.contributor.funder | Umea Plant Science Centre, Suecia | es_ES |
dc.contributor.funder | National Science Foundation, China | es_ES |
dc.contributor.funder | Knut and Alice Wallenberg Foundation | es_ES |
dc.contributor.funder | Natural Science Foundation of Fujian Province | es_ES |
dc.contributor.funder | Swedish Governmental Agency for Innovation Systems | es_ES |
dc.contributor.funder | Swedish Foundation for Humanities and Social Sciences | es_ES |
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